Arid1a (BC082554) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110030E03Rik; BAF250a; Osa1; Smarcf1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212095 representing BC082554 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCAGATGGGAAAGATGAGACCTCAGCCGTATGGTGGGACTAACCCATACTCGCAACAACAGGGAC CTCCTTCAGGACCGCAACAAGGACATGGGTACCCAGGGCAGCCATATGGGTCCCAGACTCCACAGCGGTA CCCCATGACCATGCAGGGCCGGGCTCAGAGTGCCATGGGCAGCCTCTCTTATGCACAGCAGATTCCACCT TATGGCCAGCAAGGCCCCAGTGCGTATGGCCAGCAGGGCCAGACTCCATACTATAACCAGCAAAGTCCTC ATCCCCAGCAGCAGCCACCTTACGCCCAGCAACCACCATCCCAGACCCCTCATGCCCAGCCTTCGTATCA GCAGCAGCCGCAGACTCAGCAACCACAGCTTCAGTCCTCTCAGCCTCCATATTCCCAGCAGCCATCCCAG CCTCCACATCAGCAGTCCCCAACTCCATATCCCTCCCAGCAGTCCACCACACAACAGCATCCCCAGAGCC AGCCCCCCTACTCACAACCACAGGCACAGTCTCCCTACCAGCAGCAGCAACCTCAGCAGCCAGCATCCTC GTCGCTCTCCCAGCAGGCTGCATATCCTCAGCCCCAGCCTCAGCAGTCCCAGCAAACTGCCTATTCCCAG CAGCGCTTCCCTCCACCACAGGAGCTTTCTCAAGATTCATTTGGGTCTCAGGCATCCTCAGCCCCCTCAA TGACCTCCAGTAAGGGAGGGCAAGAAGATATGAACCTGAGTCTTCAGTCAAGGCCCTCCAGCTTGCCTGA TCTGTCTGGTTCAATCGATGATCTCCCCATGGGGACAGAAGGAGCTCTGAGTCCTGGCGTGAGCACATCA GGGATTTCCAGCAGCCAAGGAGAGCAGAGCAATCCAGCTCAGTCTCCCTTTTCTCCTCACACCTCCCCTC ACCTGCCTGGCATCCGAGGCCCGTCCCCGTCCCCTGTTGGCTCTCCTGCCAGTGTCGCGCAGTCTCGCTC AGGACCACTCTCGCCTGCTGCAGTGCCAGGCAACCAGATGCCACCTCGGCCACCCAGTGGCCAGTCAGAC AGCATCATGCACCCTTCCATGAACCAATCAAGCATTGCCCAAGATCGAGGTTATATGCAGAGGAACCCCC AGATGCCCCAGTACACTTCCCCTCAGCCTGGCTCGGCCTTATCCCCACGTCAGCCGTCTGGAGGACAGAT GCACTCGGGCGTGGGCTCCTACCAGCAGAACTCCATGGGGAGCTACGGCCCCCAGGGCAGTCAGTATGGC CCACAAGGAGGCTATCCTAGGCAGCCTAACTATAATGCCTTGCCCAACGCCAACTACCCCAATGCAGGCA TGGCCGGAAGTATGAACCCTATGGGTGCTGGAGGTCAGATGCATGGGCAGCCTGGAATCCCACCTTACGG CACACTCCCTCCAGGGAGAATGGCTCATGCGTCTATGGGCAACAGGCCCTATGGCCCTAATATGGCCAAT ATGCCACCTCAGGTTGGGTCAGGGATGTGTCCTCCACCAGGGGGAATGAACAGGAAAACTCAAGAGTCTG CTGTTGCCATGCATGTTGCTGCCAACTCTATCCAAAACAGGCCACCAGGCTACCCAAATATGAATCAAGG GGGCATGATGGGAACTGGACCTCCCTATGGACAGGGGATCAATAGTATGGCTGGCATGATCAACCCTCAG GGACCCCCATATCCTATGGGTGGAACCATGGCCAACAATTCAGCAGGGATGGCAGCCAGCCCAGAGATGA TGGGCCTTGGGGATGTTAAGTTAACTCCCGCCACAAAAATGAACAACAAGGCAGATGGAACACCCAAGAC AGAATCCAAATCTAAGAAATCCAGTTCTTCTACCACCACCAATGAGAAGATCACCAAATTGTATGAGTTG GGTGGTGAGCCCGAGAGGAAGATGTGGGTGGACCGGTACCTGGCCTTCACAGAGGAGAAGGCCATGGGCA TGACAAATCTGCCTGCTGTGGGGAGGAAGCCTCTGGACCTCTATCGCCTCTATGTGTCTGTGAAGGAGAT TGGTGGGTTGACTCAGGTCAACAAGAACAAAAAATGGCGGGAACTTGCAACCAACCTCAATGTGGGTACA TCAAGCAGTGCTGCCAGCTCACTGAAAAAGCAGTATATCCAATGTCTCTATGCCTTTGAGTGCAAGATCG AGCGTGGAGAAGACCCTCCCCCCGATATCTTCGCAGCTGCTGACTCCAAGAAGTCCCAACCCAAGATCCA GCCCCCCTCTCCTGCGGGATCAGGGTCTATGCAGGGGCCACAAACTCCTCAGTCAACCAGCAGTTCTATG GCAGAAGGAGGAGACCTGAAGCCACCAACTCCAGCATCCACACCACATAGTCAAATTCCCCCCTTACCAG GCATGAGGAGCAACTCAGTCGGAATCCAGGATGCCTTTCCTGATGGAAGTGACCCCACATTCCAGAAGCG GAATTCCATGACTCCAAACCCTGGGTACCAGCCCAGTATGAATACCTCTGACATGATGGGGCGCATGTCC TATGAGCCAAATAAGGATCCTTATGGCAGCATGAGGAAAGCGCCAGGAAGTGATCCCTTCATGTCCTCAG GGCAGGGCCCCAATGGCGGGATGGGTGATCCCTACAGCCGTGCTGCTGGCCCTGGGCTGGGAAGTGTGGC GATGGGACCACGGCAGCACTATCCCTATGGAGGTCCTTACGACAGAGTGAGGACGGAGCCTGGAATCGGG CCTGAAGGAAATATGGGCACTGGAGCCCCTCAGCCAAATCTCATGCCTTCCACCCCAGATTCGGGGATGT ATTCTCCTAGCCGCTACCCCCCGCAGCAGCAGCAGCAACAGCAGCAACAGCAGCAACAGCAACGACATGA TTCCTATGGCAATCAATTCTCTACCCAAGGCACCCCTTCCAGCAGCCCCTTCCCCAGCCAGCAGACCACA ATGTATCAGCAGCAGCAGCAGAATTATAAGAGGCCAATGGATGGCACATATGGCCCCCCTGCCAAGCGGC ATGAAGGGGAGATGTACAGTGTGCCGTACAGCGCTGGGCAAGGCCAGCCTCAACAGCAGCAGTTGCCTGC AGCTCAGTCCCAGCCTGCCAGCCAGCCACAAGCTGCCCAGCCTTCCCCTCAGCAGGACGTGTACAACCAG TACAGCAATGCCTACCCTGCCTCCGCCACCGCTGCTACTGATCGCCGACCAGCAGGCGGCCCCCAGAACC AATTTCCATTCCAGTTTGGCCGAGACCGAGTCTCTGCACCTCCTGGTTCCAGTGCCCAGCAGAACATGCC ACCACAAATGATGGGTGGCCCCATACAGGCATCAGCTGAGGTTGCTCAGCAGGGCACCATGTGGCAGGGG CGAAATGACATGACCTACAATTATGCCAACAGGCAGAACACAGGCTCTGCCACCCAGGGCCCTGCGTATC ATGGTGTGAACCGAACAGATGAAATGCTCCACACAGATCAGAGGGCCAACCATGAAGGCCCATGGCCTTC CCATGGCACACGCCAGCCTCCGTATGGTCCTTCAGCCCCTGTTCCCCCCATGACAAGGCCCCCTCCATCT AACTACCAGCCCCCACCAAGCATGCCGAATCACATTCCTCAGGTATCCAGCCCCGCTCCCCTCCCCCGGC CCATGGAGAACCGTACTTCTCCTAGCAAGTCTCCATTCCTGCACTCTGGGATGAAAATGCAAAAGGCGGG TCCACCGGTGCCTGCTTCGCACATAGCGCCTACCCCTGTGCAGCCGCCTATGATTCGGCGGGATATCACC TTCCCACCTGGCTCTGTAGAGGCCACTCAGCCTGTGTTGAAGCAGAGAAGGCGGCTCACAATGAAAGACA TTGGAACCCCGGAGGCATGGCGGGTAATGATGTCCCTCAAGTCCGGGCTCCTGGCAGAGAGCACGTGGGC GTTAGACACCATTAACATTCTACTGTATGATGACAACAGCATTATGACCTTCAACCTCAGCCAGCTCCCA GGCTTGCTAGAGCTCCTTGTGGAATATTTCCGTAGATGCCTAATTGAAATCTTTGGCATTTTAAAGGAGT ATGAGGTAGGGGACCCAGGACAGAGAACATTACTAGACCCTGGGAGATTCACCAAGGTGTATAGTCCAGC CCATACAGAGGAAGAAGAGGAAGAACACCTTGATCCTAAACTGGAGGAGGAAGAGGAAGAAGGGGTTGGA AATGATGAGGAGATGGCCTTTTTGGGCAAGGACAAGCCATCTTCAGAGAATAATGAGGAGAAGCTAGTCA GTAAGTTTGACAAGCTTCCGGTAAAGATCGTGCAGAGGAATGACCCATTTGTGGTGGACTGCTCAGATAA GCTTGGGCGCGTGCAGGAGTTTGACAGTGGCCTGCTACACTGGCGGATTGGTGGTGGGGATACCACTGAG CATATCCAGACCCACTTTGAGAGCAAGATAGAGCTGCTGCCTTCCCGGCCTTATGTGCCCTGCCCAACGC CCCCTCGGAAACACCTCACAACAGTAGAGGGCACACCAGGGACAACGGAGCAGGAGGGCCCCCCGCCCGA TGGCCTTCCAGAGAAAAGGATCACAGCCACCATGGATGACATGTTGTCTACCCGGTCTAGCACATTGACT GATGAGGGGGCAAAGAGTGCAGAGGCCACCAAGGAAAGCAGCAAGTTTCCATTTGGCATTAGCCCAGCAC AGAGCCACCGGAACATCAAAATTTTAGAGGATGAACCCCATAGTAAGGATGAGACCCCACTGTGTACCCT TCTGGACTGGCAGGATTCCCTTGCTAAGCGCTGTGTCTGTGTCTCCAATACCATCCGGAGCCTGTCGTTT GTGCCAGGCAACGACTTTGAGATGTCCAAACACCCAGGGCTGCTGCTTATCCTGGGCAAGCTGATCCTGC TGCACCACAAGCACCCAGAGCGGAAGCAGGCACCACTAACTTATGAGAAGGAGGAGGAACAGGACCAAGG GGTGAGCTGTGACAAAGTGGAGTGGTGGTGGGACTGCTTGGAGATGCTCCGAGAAAACACGCTGGTCACC CTCGCCAACATCTCGGGGCAATTGGACCTATCCCCATATCCTGAGAGCATCTGCCTGCCTGTCCTGGACG GACTCCTACACTGGGCAGTTTGCCCTTCAGCTGAAGCCCAGGACCCCTTCTCAACCCTAGGCCCCAATGC CGTCCTCTCCCCCCAGAGATTGGTCTTGGAAACCCTCAGCAAACTCAGCATCCAGGACAACAATGTGGAC CTGATCCTGGCCACTCCCCCTTTTAGCCGCCTGGAGAAGTTGTATAGTACCATGGTGCGCTTCCTCAGTG ACCGAAAGAACCCAGTGTGCCGGGAGATGGCCGTGGTACTGCTGGCAAATCTGGCCCAGGGGGACAGCCT GGCAGCCCGGGCCATTGCAGTGCAGAAGGGCAGCATCGGCAACCTCCTGGGTTTCCTGGAGGACAGCCTT GCTGCCACACAGTTCCAGCAGAGCCAGGCAAGCCTCCTGCATATGCAGAATCCACCCTTTGAACCAACTA GTGTGGACATGATGCGGCGGGCTGCCCGAGCACTGCTTGCCCTGGCCAAGGTGGATGAGAACCACTCAGA GTTCACTCTGTATGAGTCACGGCTGTTGGACATCTCCGTGTCACCACTGATGAACTCATTGGTTTCACAA GTCATTTGTGATGTACTGTTTTTGATTGGCCAGTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212095 representing BC082554 Red=Cloning site Green=Tags(s) MDQMGKMRPQPYGGTNPYSQQQGPPSGPQQGHGYPGQPYGSQTPQRYPMTMQGRAQSAMGSLSYAQQIPP YGQQGPSAYGQQGQTPYYNQQSPHPQQQPPYAQQPPSQTPHAQPSYQQQPQTQQPQLQSSQPPYSQQPSQ PPHQQSPTPYPSQQSTTQQHPQSQPPYSQPQAQSPYQQQQPQQPASSSLSQQAAYPQPQPQQSQQTAYSQ QRFPPPQELSQDSFGSQASSAPSMTSSKGGQEDMNLSLQSRPSSLPDLSGSIDDLPMGTEGALSPGVSTS GISSSQGEQSNPAQSPFSPHTSPHLPGIRGPSPSPVGSPASVAQSRSGPLSPAAVPGNQMPPRPPSGQSD SIMHPSMNQSSIAQDRGYMQRNPQMPQYTSPQPGSALSPRQPSGGQMHSGVGSYQQNSMGSYGPQGSQYG PQGGYPRQPNYNALPNANYPNAGMAGSMNPMGAGGQMHGQPGIPPYGTLPPGRMAHASMGNRPYGPNMAN MPPQVGSGMCPPPGGMNRKTQESAVAMHVAANSIQNRPPGYPNMNQGGMMGTGPPYGQGINSMAGMINPQ GPPYPMGGTMANNSAGMAASPEMMGLGDVKLTPATKMNNKADGTPKTESKSKKSSSSTTTNEKITKLYEL GGEPERKMWVDRYLAFTEEKAMGMTNLPAVGRKPLDLYRLYVSVKEIGGLTQVNKNKKWRELATNLNVGT SSSAASSLKKQYIQCLYAFECKIERGEDPPPDIFAAADSKKSQPKIQPPSPAGSGSMQGPQTPQSTSSSM AEGGDLKPPTPASTPHSQIPPLPGMRSNSVGIQDAFPDGSDPTFQKRNSMTPNPGYQPSMNTSDMMGRMS YEPNKDPYGSMRKAPGSDPFMSSGQGPNGGMGDPYSRAAGPGLGSVAMGPRQHYPYGGPYDRVRTEPGIG PEGNMGTGAPQPNLMPSTPDSGMYSPSRYPPQQQQQQQQQQQQQRHDSYGNQFSTQGTPSSSPFPSQQTT MYQQQQQNYKRPMDGTYGPPAKRHEGEMYSVPYSAGQGQPQQQQLPAAQSQPASQPQAAQPSPQQDVYNQ YSNAYPASATAATDRRPAGGPQNQFPFQFGRDRVSAPPGSSAQQNMPPQMMGGPIQASAEVAQQGTMWQG RNDMTYNYANRQNTGSATQGPAYHGVNRTDEMLHTDQRANHEGPWPSHGTRQPPYGPSAPVPPMTRPPPS NYQPPPSMPNHIPQVSSPAPLPRPMENRTSPSKSPFLHSGMKMQKAGPPVPASHIAPTPVQPPMIRRDIT FPPGSVEATQPVLKQRRRLTMKDIGTPEAWRVMMSLKSGLLAESTWALDTINILLYDDNSIMTFNLSQLP GLLELLVEYFRRCLIEIFGILKEYEVGDPGQRTLLDPGRFTKVYSPAHTEEEEEEHLDPKLEEEEEEGVG NDEEMAFLGKDKPSSENNEEKLVSKFDKLPVKIVQRNDPFVVDCSDKLGRVQEFDSGLLHWRIGGGDTTE HIQTHFESKIELLPSRPYVPCPTPPRKHLTTVEGTPGTTEQEGPPPDGLPEKRITATMDDMLSTRSSTLT DEGAKSAEATKESSKFPFGISPAQSHRNIKILEDEPHSKDETPLCTLLDWQDSLAKRCVCVSNTIRSLSF VPGNDFEMSKHPGLLLILGKLILLHHKHPERKQAPLTYEKEEEQDQGVSCDKVEWWWDCLEMLRENTLVT LANISGQLDLSPYPESICLPVLDGLLHWAVCPSAEAQDPFSTLGPNAVLSPQRLVLETLSKLSIQDNNVD LILATPPFSRLEKLYSTMVRFLSDRKNPVCREMAVVLLANLAQGDSLAARAIAVQKGSIGNLLGFLEDSL AATQFQQSQASLLHMQNPPFEPTSVDMMRRAARALLALAKVDENHSEFTLYESRLLDISVSPLMNSLVSQ VICDVLFLIGQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC082554
ORF Size	5706 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC082554.1
RefSeq Size	6487 bp
RefSeq ORF	5708 bp
Locus ID	93760
MW	237.8 kDa
Gene Summary	Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (PubMed:17640523).[UniProtKB/Swiss-Prot Function]

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