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Ankle1 (NM_172756) Mouse Tagged ORF Clone
CAT＃: MR212522
- TrueORF®
Ankle1 (Myc-DDK-tagged) - Mouse ankyrin repeat and LEM domain containing 1 (Ankle1)
ORF Plasmid: tGFP
"NM_172756" in other vectors (4)
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CNY 3,976.00
CNY 4,660.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 8430438L13Rik; Ankrd41; C430003I23; Lem3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212522 representing NM_172756
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGATACTGCATGCTTGGCTCTGAGGCTGCTAGCAGCACTACGCGAGGAGGAGGCGCGGGCTGTGG AGGAGCTGCTGCGCCTCGGAGCGGACCCCAACCTGGTGTTGGACGATGGCGCTGCGGCGGTGCACCTGGC AGCCAGAGCGAGCCACCCGCGCGCCCTGCATTGCCTTCGGATGCTGCTGCGCTGGGGAGCGGATCCCAAT GCTCGGTCCGCGGAGGGGCTGACGCCTGTGCATGTGGCTGCCGCGTGGGGCTGTTGTGGTGCTCTGGAGT TGCTGCTGAGCCGAGGAGGTGACCCCACACTGCGGGACCAGGATGGGCTCAGGCCCTTGGACTGGGCACT ACAGCAGCGACACCACAACTGCGCACGAGTGCTGCAGGAGCTGGACACGCCCACCCAGCCTGATGAGACC CGAGAACCCACAGAGACGTTTCATGTAGCCCAGGGTAGCTTTGAAACTGAAACATGCCAAGGACCTGCCT TAGCAGAGTCTTCTGGAGTTTCTCAAGATTCGGAGTTGCACGTGCATAGAGCTGAGCTGGAGGTGGAGGC TGTAGAGGTGGCTGTGCATCCCCAGAGCTCTGAGGCCACCGAGAACTCAGACTACAGCAGCGATGCTTCG TTTGTCACTGCAGTGGAGGACTCTCTGCAGCCCGGGCGCCCTGGGGGGGCATTGGAGCTAGTGGCTGGCT TGTGGGTAACCCGTGGGGCTGTGTCAGCTGGGAAGGGAGCACCCAACTGCCAGCCGCAGGTCCTGACATT GACTGCAAGGGACACGGACAAACCCGTACTGCCTGGTGATGGGGATCTGGGGGCTTTGCACCCCCACAGT TCGGTGCCCCCCATGTCGGATCTGCAGCTCCTCCAGGCCCTGCGGGCGCTGGGCTATAGCCCTGGACCCG TCACACCTTTCACCAGAGGACACTATCTACGGCGGCTGCAGGAAGCCCAGGCTAGCCGTGCTGATGTAGG GCATAGCCAAGAACTGGCGGAGGCACTAAGGACAGGCACCATCCCTGACTGCCAGGTGGATGAGGAAGCC CTGGCTCAGTGCTTTCAGCGGCTGGATCCCTTGAAGAAGTGGCGGGAAGGCATTACAAAGTCTAGTTTTA CGTATCTACTTCTCGACCCCAGGCTGACGAAGGACCTGCCGGCCCGAGCCTCTTCCCTCACACTGGCTGA GTGTCTGCAATGTTTCGTGAGAGCCATCTTCTATGTGGGCAAGGGAACCCGAGCCCGACCAGATGCCCAC CTCTGGGAAGCCTTTGGCTACCATGACCAGCCAAGGAAACAGGTCTGTCCCAAGGTTCGCCGGATCTTAG ACATCTGGGCTAGTGGCCGGGGCATCATCTCTCTGCACTGCTTCCAGCATGTGGTGGCCATGGAAGCGTA CACTCGGGAAGCCTGTCTGTTGGATGCCCTAGGTCTCCAGACACTGACCAACCAGAAACAAGGGCACTAC TATGGAGTGGTTGCCCATTGGCCACCCTCTCGGCGTCGGCGTTTGGGGGTGCACCTGCTACAGCGTGCTC TGCTGGTCTTCCTGGCTGAGGGGGAGAGAGAGCTGAGGCCTCAGGACATTCAGGCTCGAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212522 representing NM_172756
Red=Cloning site Green=Tags(s) MADTACLALRLLAALREEEARAVEELLRLGADPNLVLDDGAAAVHLAARASHPRALHCLRMLLRWGADPN ARSAEGLTPVHVAAAWGCCGALELLLSRGGDPTLRDQDGLRPLDWALQQRHHNCARVLQELDTPTQPDET REPTETFHVAQGSFETETCQGPALAESSGVSQDSELHVHRAELEVEAVEVAVHPQSSEATENSDYSSDAS FVTAVEDSLQPGRPGGALELVAGLWVTRGAVSAGKGAPNCQPQVLTLTARDTDKPVLPGDGDLGALHPHS SVPPMSDLQLLQALRALGYSPGPVTPFTRGHYLRRLQEAQASRADVGHSQELAEALRTGTIPDCQVDEEA LAQCFQRLDPLKKWREGITKSSFTYLLLDPRLTKDLPARASSLTLAECLQCFVRAIFYVGKGTRARPDAH LWEAFGYHDQPRKQVCPKVRRILDIWASGRGIISLHCFQHVVAMEAYTREACLLDALGLQTLTNQKQGHY YGVVAHWPPSRRRRLGVHLLQRALLVFLAEGERELRPQDIQARG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172756 |
| ORF Size | 1602 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172756.3, NP_766344.2 |
| RefSeq Size | 2772 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 234396 |
| UniProt ID | A8VU90 |
| MW | 58.4 kDa |
| Gene Summary | Endonuclease that probably plays a role in the DNA damage response and DNA repair.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217650 | Ankle1 (untagged) - Mouse ankyrin repeat and LEM domain containing 1 (Ankle1), (10ug) |
CNY 6,840.00 |
|
| MG212522 | Ankle1 (tGFP-tagged) - Mouse ankyrin repeat and LEM domain containing 1 (Ankle1), (10ug) |
CNY 5,130.00 |
|
| MR212522L3 | Lenti ORF clone of Ankle1 (Myc-DDK-tagged) - Mouse ankyrin repeat and LEM domain containing 1 (Ankle1) |
CNY 6,560.00 |
|
| MR212522L4 | Lenti ORF clone of Ankle1 (mGFP-tagged) - Mouse ankyrin repeat and LEM domain containing 1 (Ankle1) |
CNY 6,560.00 |
