Rbmx (NM_011252) Mouse Tagged ORF Clone
CAT#: MR215210
- TrueORF®
Rbmx (Myc-DDK-tagged) - Mouse RNA binding motif protein, X chromosome (Rbmx), transcript variant 1
ORF Plasmid: tGFP
"NM_011252" in other vectors (5)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | hnRNP G |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215210 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGAAGCAGATCGCCCAGGAAAGCTCTTTATTGGTGGGCTTAATACAGAGACGAATGAGAAAGCCC TTGAGGCAGTGTTTGGCAAATATGGACGAATAGTGGAAGTTCTTTTGATGAAGGACCGAGAAACGAATAA GTCAAGAGGATTCGCTTTTGTCACTTTTGAAAGCCCAGCAGATGCAAAGGATGCTGCCAGAGACATGAAT GGAAAGTCCTTAGATGGGAAAGCCATCAAGGTGGAGCAAGCCACCAAACCATCATTTGAAAGTGGTAGAC GTGGACTACCCCCACCTCCAAGAAGCAGAGGCCCTCCCAGAGGTCTTCGAGGAGGAAGAGGAGGAAGTGG AGGAACCAGAGGACCCCCCTCCCGGGGAGGACACATGGATGATGGTGGCTACTCCATGAATTTTACCATG AGTTCTTCCAGAGGACCTCTGCCAGTAAAACGAGGACCACCACCACGAAGTGGAGGTCCTCCTCCTAAAA GATCAGCACCTTCTGGACCAGTTCGTAGCAGCAGTGGACTGGGAGGAAGAGCCCCTGTGTCCCGTGGAAG AGATGGTTACGGAGGCCCGCCACGAAGGGAGCCCCTGCCTTCTCGAAGAGATGTTTATTTGTCCCCGAGA GATGATGGATATTCCACTAAAGACAGCTATTCAAGCAGAGAATATCCAAGTTCTCGAGATACACGAGATT ATGCACCACCACCAAGAGATTATACTTACCGTGATTATGGTCATTCCAGTTCACGAGATGACTATCCATC AAGAGGCTATAGTGATAGAGATGGCTATGGTCGGGATCGGGACTATTCAGATCATCCAAGTGGAGGTTCC TACAGAGATTCGTATGAGAGTTATGGTAACTCACGTAGTGCTCCACCTACACGAGGGCCCCCGCCATCTT ATGGTGGAAGCAGTCGCTATGATGATTACAGCAGCTCACGTGACGGATATGGTGGAAGTCGAGACAGTTA CTCAAGCAGCCGAAGTGATCTCTACTCAAGTGGTCGTGATCGCGTGGGCAGACAAGAAAGAGGGCTTCCC CCTTCTATGGAAAGGGGGTACCCTCCTCCACGTGATTCCTACAGCAGTTCAAGCCGCGGAGCACCAAGAG GTGGTGGCCGTGGAGGAAGCCGATCTGATAGAGGGGGAGGCAGAAGCAGATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215210 protein sequence
Red=Cloning site Green=Tags(s) MVEADRPGKLFIGGLNTETNEKALEAVFGKYGRIVEVLLMKDRETNKSRGFAFVTFESPADAKDAARDMN GKSLDGKAIKVEQATKPSFESGRRGLPPPPRSRGPPRGLRGGRGGSGGTRGPPSRGGHMDDGGYSMNFTM SSSRGPLPVKRGPPPRSGGPPPKRSAPSGPVRSSSGLGGRAPVSRGRDGYGGPPRREPLPSRRDVYLSPR DDGYSTKDSYSSREYPSSRDTRDYAPPPRDYTYRDYGHSSSRDDYPSRGYSDRDGYGRDRDYSDHPSGGS YRDSYESYGNSRSAPPTRGPPPSYGGSSRYDDYSSSRDGYGGSRDSYSSSRSDLYSSGRDRVGRQERGLP PSMERGYPPPRDSYSSSSRGAPRGGGRGGSRSDRGGGRSRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011252 |
ORF Size | 1176 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011252.4, NP_035382.1 |
RefSeq Size | 2437 bp |
RefSeq ORF | 1176 bp |
Locus ID | 19655 |
UniProt ID | Q9WV02 |
MW | 42.3 kDa |
Gene Summary | RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205665 | Rbmx (untagged) - Mouse RNA binding motif protein, X chromosome (Rbmx), transcript variant 1, (10ug) |
CNY 3,656.00 |
|
MG206105 | Rbmx (tGFP-tagged) - Mouse RNA binding motif protein, X chromosome (Rbmx) |
CNY 3,140.00 |
|
MG215210 | Rbmx (tGFP-tagged) - Mouse RNA binding motif protein X chromosome (Rbmx) transcript variant 1, (10ug) |
CNY 3,140.00 |
|
MR215210L3 | Lenti ORF clone of Rbmx (Myc-DDK-tagged) - Mouse RNA binding motif protein, X chromosome (Rbmx), transcript variant 1 |
CNY 4,750.00 |
|
MR215210L4 | Lenti ORF clone of Rbmx (mGFP-tagged) - Mouse RNA binding motif protein, X chromosome (Rbmx), transcript variant 1 |
CNY 4,750.00 |