Arhgef5 (NM_133674) Mouse Tagged Lenti ORF Clone
CAT#: MR215297L4
- LentiORF®
Lenti ORF clone of Arhgef5 (mGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5)
"NM_133674" in other vectors (6)
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CNY 13,970.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | 2210412D05Rik; AA717842; AW495314; Tim1 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR215297).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133674 |
ORF Size | 4743 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133674.1, NP_598435.1 |
RefSeq Size | 5420 bp |
RefSeq ORF | 4746 bp |
Locus ID | 54324 |
UniProt ID | E9Q7D5 |
Gene Summary | Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:19713215, PubMed:21525037). Strongly activates RHOA (PubMed:19713215, PubMed:21525037). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (PubMed:19713215). Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:21525037). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (By similarity). Required for SRC-induced podosome formation (PubMed:21525037). Involved in positive regulation of immature dendritic cell migration (PubMed:19713215).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224710 | Arhgef5 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5), (10ug) |
CNY 20,330.00 |
|
MG215297 | Arhgef5 (tGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5), (10ug) |
CNY 13,300.00 |
|
MR215297 | Arhgef5 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5) |
CNY 17,304.00 |
|
MR215297L1 | Lenti ORF clone of Arhgef5 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5) |
CNY 13,970.00 |
|
MR215297L2 | Lenti ORF clone of Arhgef5 (mGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5) |
CNY 19,704.00 |
|
MR215297L3 | Lenti ORF clone of Arhgef5 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5) |
CNY 13,970.00 |
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