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Tlk2 (NM_001112705) Mouse Tagged ORF Clone
CAT＃: MR215864
- TrueORF®
Tlk2 (Myc-DDK-tagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant A
ORF Plasmid: tGFP
"NM_001112705" in other vectors (4)
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CNY 5,496.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4933403M19Rik; PKU-alpha; PKUalpha; Tlk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215864 representing NM_001112705
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAAGAACTGCATAGCCTGGACCCACGAAGGCAGGAATTACTAGAGGCCAGGTTCACTGGAGTTG GTGTAAGTAAGGGGCCACTCAACAGTGAGTCTTCCAACCAGAGTCTGTGCAGCGTGGGGTCGTTGAGTGA TAAAGAAGTAGAGACTCCTGAGAAAAAGCAGAATGACCAGCGAAATCGGAAAAGGAAAGCCGAGCCATAT GACACTAGCCAAGGGAAAGGCACTCCTAGGGGACATAAAATTAGTGATTACTTTGAGTTTGCTGGGGGAA GCGGGCCAGGAACCAGCCCTGGCAGAAGTGTTCCACCAGTTGCACGATCCTCACCGCAACATTCCTTATC CAATCCCTTACCGCGTCGAGCAGAACAGCCTCTGTATGGTTTAGATGGCAGTGCAGCAAAGGAGGCCTCA GAAGAGCAGTCTGCTCTGCCCACCCTCATGTCAGTGATGTTAGCAAAACCGCGACTTGACACAGAGCAGT TAGCGCCACGGGGAGCTGGCCTCTGCTTCACTTTCGTCTCTGCTCAACAAAACAGTCCTTCTTCTACGGG TTCTGGCAATACAGAACATTCTTGCAGCTCCCAAAAACAGATCTCCATCCAGCACAGACAGACCCAGTCT GACCTCACAATAGAAAAAATATCTGCACTAGAAAACAGTAAGAACTCTGACTTAGAGAAGAAGGAAGGAA GAATAGATGATTTATTAAGAGCCAACTGTGATTTGAGACGACAGATAGATGAACAGCAAAAGATGCTAGA GAAATACAAGGAACGATTAAATAGATGTGTCACCATGAGCAAGAAGCTCCTTATAGAAAAGTCAAAACAA GAGAAGATGGCGTGCAGAGATAAGAGCATGCAGGACCGATTGCGATTAGGCCACTTTACTACTGTCCGGC ATGGAGCCTCGTTTACTGAGCAGTGGACAGATGGTTATGCTTTCCAAAACCTCATCAAGCAACAGGAAAG GATAAATTCACAGAGAGAAGAGATAGAAAGGCAACGGAAAATGTTAGCAAAACGGAAACCTCCTGCCATG GGTCAGGCCCCTCCTGCAACCAATGAGCAGAAACAACGGAAAAGCAAGACTAATGGAGCTGAAAATGAAA CGTTAACGTTAGCCGAGTACCATGAACAAGAGGAAATCTTCAAACTTAGATTAGGTCATCTTAAGAAAGA GGAAGCAGAAATCCAGGCAGAGCTGGAAAGGCTGGAAAGGGTTAGGAATCTACACATCAGGGAATTAAAA AGGATACATAATGAAGACAATTCGCAGTTTAAAGACCATCCAACACTAAATGACAGATATTTGTTGTTAC ATCTTTTGGGTAGAGGAGGTTTCAGTGAAGTTTACAAGGCATTTGATCTAACGGAGCAAAGATATGTAGC TGTGAAAATTCACCAGTTAAATAAAAACTGGAGAGATGAGAAAAAGGAGAATTACCACAAGCATGCGTGT AGGGAATACCGGATTCACAAGGAGCTGGACCACCCCAGGATAGTGAAGCTGTATGATTACTTTTCACTGG ACACTGACTCGTTTTGTACAGTATTAGAATACTGTGAAGGGAATGACCTGGACTTCTACCTAAAACAGCA CAAATTAATGTCGGAGAAAGAAGCCCGATCCATTATTATGCAGATTGTGAATGCTTTAAAGTACTTAAAT GAAATAAAACCTCCCATCATACACTATGACCTCAAACCAGGTAATATCCTTTTAGTAAATGGTACAGCAT GTGGAGAGATAAAAATTACAGATTTTGGTCTTTCCAAGATCATGGATGATGATAGCTACAATTCAGTGGA TGGCATGGAGCTGACGTCACAAGGAGCTGGTACTTATTGGTATTTACCACCAGAGTGTTTTGTGGTTGGG AAAGAGCCACCAAAGATCTCAAATAAAGTCGATGTTTGGTCAGTGGGTGTGATCTTCTACCAGTGTCTTT ATGGGAGGAAGCCTTTTGGCCATAACCAGTCCCAGCAAGATATTCTACAAGAGAATACTATTCTTAAGGC TACTGAAGTACAGTTCCCGCCAAAGCCAGTAGTAACACCTGAAGCAAAGGCATTTATCAGGAGATGTCTG GCCTATCGAAAGGAAGATCGCATTGATGTGCAGCAGCTGGCCTGTGACCCCTACTTGTTGCCTCACATCC GAAAGTCAGTCTCCACAAGTAGCCCTGCAGGAGCTGCTATTGCATCAACCTCTGGGGCATCCAATAACAG TTCTTCGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215864 representing NM_001112705
Red=Cloning site Green=Tags(s) MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPY DTSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPRRAEQPLYGLDGSAAKEAS EEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQS DLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQ EKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAM GQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELK RIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHAC REYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLN EIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVG KEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCL AYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001112705 |
| ORF Size | 2250 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001112705.2, NP_001106176.1 |
| RefSeq Size | 5475 bp |
| RefSeq ORF | 2253 bp |
| Locus ID | 24086 |
| MW | 85.8 kDa |
| Gene Summary | Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (By similarity). Negative regulator of amino acid starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221296 | Tlk2 (untagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant A, (10ug) |
CNY 9,690.00 |
|
| MG215864 | Tlk2 (tGFP-tagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2) transcript variant A, (10ug) |
CNY 6,370.00 |
|
| MR215864L3 | Lenti ORF clone of Tlk2 (Myc-DDK-tagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant A |
CNY 7,700.00 |
|
| MR215864L4 | Lenti ORF clone of Tlk2 (mGFP-tagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant A |
CNY 7,700.00 |
