Nek1 (NM_175089) Mouse Tagged ORF Clone
CAT#: MR216282
- TrueORF®
Nek1 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1)
ORF Plasmid: tGFP
"NM_175089" in other vectors (4)
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CNY 8,376.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D8Ertd790e; kat |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216282 representing NM_175089
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGTATGTGAGACTGCAGAAGATTGGAGAAGGTTCATTTGGAAAAGCTGTTCTTGTTAAATCGA CAGAGGATGGCAGACATTATGTCATCAAGGAAATTAACATCTCAAGAATGTCTGATAAAGAAAGGCAAGA ATCAAGGAGAGAAGTTGCTGTATTGGCAAACATGAAGCATCCAAATATTGTCCAATATAAAGAATCATTT GAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAAGGAGGTGATTTGTTTAAACGAATAAATG CTCAGAAAGGCGCTCTGTTTCAAGAAGACCAGATTTTGGACTGGTTTGTGCAGATATGTTTGGCTCTGAA GCATGTACATGATAGAAAAATTCTTCACCGAGACATAAAGTCACAGAACATATTTCTAACCAAAGATGGG ACAGTGCAGCTTGGAGATTTTGGAATTGCTCGAGTTCTTAATAGTACTGTAGAGCTGGCTCGAACTTGCA TAGGCACTCCATACTACTTGTCACCTGAAATCTGTGAAAACAAGCCTTATAACAATAAAAGTGACATTTG GGCTTTGGGCTGTGTCCTTTATGAGTTGTGTACACTTAAACATGCATTTGAAGCTGGAAACATGAAAAAC CTGGTACTGAAGATAATCTCCGGATCCTTTCCTCCAGTGTCTCCACATTACTCCTATGATCTCCGCAGCT TGCTGTCTCAGTTATTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGTTT TATAGCTAAACGAATCGAAAAGTTTCTCTCCCCTCAGCTTATTGCAGAAGAATTTTGTCTAAAAACACTT TCAAAGTTTGGACCACAGCCTCTCCCAGGTAAAAGACCAGCATCAGGACAAGGTGTCAGTTCTTTTGTCC CTGCTCAGAAAATCACAAAGCCTGCTGCTAAATACGGAGTGCCTTTAACATATAAGAAGTATGGAGATAA AAAGTTACTTGAGAAAAAACCACCCCCAAAACATAAACAGGCCCATCAAATTCCCGTGAAGAAAATGAAT TCTGGAGAAGAAAGGAAGAAAATGTCTGAGGAAGCAGCAAAAAAAAGAAGGTTGGAATTTATTGAGAAAG AAAAGAAGCAAAAGGATCAGATTAGGTTCCTGAAGGCTGAGCAGATGAAGCGGCAAGAGAAGCAGCGGTT GGAGAGGATAAATAGGGCCAGGGAACAAGGATGGAGGAATGTTTTAAGGGCTGGTGGAAGCGGTGAAGTA AAGGCTTCCTTTTTTGGCATTGGAGGGGCTGTCTCTCCATCACCGTGTTCTCCTCGAGGCCAGTATGAAC ATTACCATGCCATTTTTGACCAAATGCAGCGGCTAAGAGCAGAAGATAATGAAGCAAGATGGAAGGGGGG AATCTATGGTCGATGGCTCCCAGAAAGGCAAAAAGGACACTTAGCTGTAGAGAGAGCCAACCAAGTGGAA GAATTCCTACAGCGTAAACGAGAAGCTATGCAGAATAAAGCCCGAGCCGAAGGACACGTGGTTTATTTGG CAAGACTGAGGCAAATAAGACTACAAAATTTTAATGAGCGCCAACAGATTAAAGCCAAACTTCGTGGTGA GAATAAAGAAGCTGATGGTACCAAAGGACAAGAAGCAACTGAAGAGACTGACATGAGGCTCAAAAAGATG GAGTCACTTAAGGCGCAAACAAATGCACGTGCTGCTGTACTAAAAGAACAGCTGGAGCGAAAAAGAAAGG AAGCTTATGAAAGAGAAAAGAAAGTATGGGAAGAACATTTGGTGGCGAGGGTAAAAAGCTCAGATGTTCC TCTGCCTTTGGAACTTCTTGAAACAGGTGGTTCTCCATCAAAGCAGCAGGTGAAGCCTGTCATTTCTGTG ACTTCAGCTTTGAAAGAAGTGGGCCTGGATGGAAGTTTAACTGATACCCAGGAAGAAGAAATGGAAAAGA GTAACAGTGCTATTTCAAGTAAGCGAGAAATCCTGCGTAGGCTAAATGAAAATCTTAAAGCTCAAGAGGA TGAAAAGGAAAAGCAGCATCACTCAGGTTCTTGTGAGACCGTTGGTCACAAAGATGAGAGAGAGTATGAG ACAGAAAATGCCATTTCCTCTGATCGCAAGAAGTGGGAGATGGGAGGTCAGCTTGTGATTCCTCTCGATG CAGTGACACTGGATACATCCTTCTCTGCAACCGAAAAACATACTGTGGGAGAGGTTATTAAATTAGATTC TAATGGCTCTCCAAGAAAAGTCTGGGGGAAAAACCCTACAGATTCTGTGCTGAAGATACTTGGAGAAGCT GAATTACAGCTACAGACAGAACTACTAGAAAACACATCTTTTAAAAGTGAGGTTTATGCTGAAGAGGAGA ACTACAAACCCTTACTTACTGAAGAAGAGAATCTGCAGTGCATTTCAAAAGAAATAAATCCATCAGCTAC TGTTGATTCTACTGAAACGAAAAGTCCAAAGTTTACTGAGGTGTCTCCACAAATGTCAGAAGGAAATGTG GAAGAACCTGATGATTTGGAAACAGAAGTTCTACAAGAGCCAAGTAGCACACACACAGATGGGAGTTTGC CACCTGTTCTTAATGATGTGTGGACTAGAGAGAAGGAAGCAGCTAAGGAAACTGAGTTGGAAGATAAGGT TGCTGTGCAGCAGAGTGAAGTTTGTGAAGATAGAATTCCAGGGAACGTGGACCAATCCTGTAAGGATCAG AGAGATCCTGCAGTAGACGATTCTCCGCAGTCTGGCTGTGATGTAGAGAAGTCAGTACAGCCAGAATCGA TTTTCCAGAAAGTGGTTCATTCTAAGGACTTGAACTTAGTTCAGGCAGTTCATTGCTCACCAGAAGAACC AATTCCAATTCGATCTCACTCTGATTCTCCACCAAAAACTAAGAGCAAGAATTCCTTACTGATTGGACTT TCAACTGGTCTGTTTGATGCAAACAATCCAAAGATGCTGAGGACCTGCTCACTTCCAGATCTTTCCAAGC TGTTCAGAACCCTAATGGACGTTCCCACTGTGGGGGACGTTCATCAAGACAGTCTTGAAATCGATGAGCT GGAAGATGAACCAATTAAAGAAGGGCCTTCTGATTCCGAAGACACTGTATTTGAAGAAACTGACACAGAT TTACAAGAGCTTCAGGCCTCAATGGAGCAGCTGCTTAGGGAGCAACCAGGTGACGAATACAGTGAGGAGG AAGAGTCTGTTTTAAAAAGCAGCGATGTGGAGCAGACAGCAAGAGGGACAGATGCCCCAGACGAGGAGGA CAACCCCAGCAGCGAAAGCGCCCTGAACGAGGAATGGCACTCAGATAATAGTGACGCTGAGACCACTAGT GAATGTGAATATGACAGTGTCTTTAACCATTTAGAGGAACTAAGACTTCACTTGGAGCAAGAAATGGGCT TTGAAAAGTTCTTTGAGGTTTATGAGAAAGTAAAGGCTATTCATGAGGATGAAGATGAAAATATTGAAAT TTGTTCAACAATAGTTGAGAATATTTTGGGCAATGAGCACCAGCATCTCTATGCCAAGATTCTGCATTTA GTCATGGCAGATGGAGCCTATCAGGAAGATAATGATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216282 representing NM_175089
Red=Cloning site Green=Tags(s) MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESF EENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDG TVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKN LVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTL SKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMN SGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEV KASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERQKGHLAVERANQVE EFLQRKREAMQNKARAEGHVVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKM ESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISV TSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYE TENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEA ELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNV EEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQ RDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGL STGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTD LQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTS ECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHL VMADGAYQEDNDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_175089 |
ORF Size | 3609 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175089.4, NP_780298.2 |
RefSeq Size | 4264 bp |
RefSeq ORF | 3612 bp |
Locus ID | 18004 |
UniProt ID | P51954 |
MW | 137.1 kDa |
Gene Summary | Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity (PubMed:1382974). Involved in DNA damage checkpoint control and for proper DNA damage repair (PubMed:18843199). In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (By similarity). May be implicated in the control of meiosis (PubMed:1382974). Involved in cilium assembly (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
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NEK1 Phosphorylation of YAP Promotes Its Stabilization and Transcriptional Output
,Khalil, MI;Ghosh, I;Singh, V;Chen, J;Zhu, H;De Benedetti, A;,
Cancers
,PubMed ID 33297404
[NEK1]
|
The TLK1/Nek1 axis contributes to mitochondrial integrity and apoptosis prevention via phosphorylation of VDAC1
,Singh, V;Khalil, MI;De Benedetti, A;,
Cell Cycle
,PubMed ID 31914854
[NEK1]
|
Identification of the proteome complement of humanTLK1 reveals it binds and phosphorylates NEK1 regulating its activity
,Singh, V;Connelly, ZM;Shen, X;De Benedetti, A;,
Cell Cycle
,PubMed ID 28426283
[NEK1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223912 | Nek1 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), (10ug) |
CNY 15,390.00 |
|
MG216282 | Nek1 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), (10ug) |
CNY 10,550.00 |
|
MR216282L3 | Lenti ORF clone of Nek1 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1) |
CNY 11,500.00 |
|
MR216282L4 | Lenti ORF clone of Nek1 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1) |
CNY 11,500.00 |