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Dach1 (NM_007826) Mouse Tagged ORF Clone
CAT＃: MR216327
- TrueORF®
Dach1 (Myc-DDK-tagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 1
ORF Plasmid: tGFP
"NM_007826" in other vectors (4)
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CNY 7,510.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Dac; Dach |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216327 representing NM_007826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCGATCTCTACTTCTG CTTCCTCCTCGGGCACCACCACCTCCACCTCCTCGGCGACCTCGTCTCCGGCTCCATCCATCGGACCCCC GGCGTCGTCTGGGCCAACTCTGTTCCGGCCGGAGCCCATTGCCTCTTCTGCTTCTTCTTCAGCCGCGGCC ACAGTCACCTCTCCTGGTGGCGGCGGCGGCGGCAGCGGAGGCGGCGGTGGCAGCGGCGGCAACGGAGGCG GCGGCGGGAGCAACTGCAACCCCAGCCTGGCGGCCGGGAGCAGCGGCGGCGGCGTTAGCGCTGGCGGCGG CGGCGCCTCCAGCACCCCCATCACCGCGAGCACCGGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGT AGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCTGCGGCCCCCTCCCTGGGAAAC CCGTGTACTCAACCCCGTCCCCAGTGGAAAACACCCCCCAGAATAATGAGTGCAAAATGGTGGATCTGAG AGGGGCCAAAGTGGCTTCCTTTACGGTGGAGGGCTGCGAGCTGATCTGCCTGCCCCAGGCTTTCGACCTG TTCCTGAAGCACTTGGTGGGGGGCTTGCACACCGTCTACACCAAGCTGAAGCGGTTGGAGATCACGCCGG TGGTGTGCAATGTGGAACAGGTTCGCATCCTGAGGGGACTGGGGGCCATCCAGCCCGGAGTGAACCGCTG CAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGACTGCACCAACGCCAGTTCCAGACCTGGA AGGCCTCCTAAGAGGACTCAAAGTGTCACTTCCCCAGAGAACTCTCACATCATGCCGCATTCTGTCCCTG GCCTCATGTCTCCTGGAATCATTCCACCAACAGGTCTGACTGCAGCTGCTGCAGCTGCTGCAGCTGCTAC CAATGCAGCTATTGCTGAAGCAATGAAGGTGAAAAAAATAAAATTAGAAGCTATGAGCAACTATCATGCC AGTAACAACCAACATGGAGCAGATTCTGAAAACGGGGACATGAATTCAAGTGTTGGCAGCAGTGGTGGTT CTTGGGATAAGGAAACACTGCACTCTCCCCCATCCCAGGGATCCCAGGCTCCTGTTGCACATGCCCGCAT GCCTGCAGCGTTTAGCCTTCCAGTTAGCCATCCTCTCAACCATCTGCAGCACAGCCACCTTCCGCCAAAT GGACTGGAACTTCCTTTTATGATGATGCCCCACCCTCTCATTCCTGTCAGCCTACCTCCAGCATCTGTCA CCATGGCAATGAGTCAGATGAACCACCTTAGCACCATTGCAAATATGGCGGCGGCAGCACAAGTTCAGAG TCCTCCATCCAGGGTGGAGACATCTGTTATTAAGGAGCGTGTTCCCGACAGTCCCTCGCCTGCTCCATCT CTGGAGGAGGGCCGGAGGCCCGGCAGCCACCCATCCTCACACCGCAGCAGCAGTGTGTCCAGCTCCCCGG CGCGGACTGAGAGTTCTTCCGACAGAATCCCTGTCCATCAGAATGGCCTGTCCATGAACCAGATGCTTAT GGGTTTATCCCCAAATGTGCTTCCTGGGCCAAAGGAGGGGGATTTGGCTGGTCATGACATGGGGCATGAG TCAAAACGGATCCACATTGAAAAAGATGAGACCCCACTTTCCACACCAACCGCAAGAGACAGCATCGACA AACTTTCTCTAACTGGGCATGGACAACCACTACCTCCCGGCTTCCCATCTCCCTTTCTGTTTCCTGATGG CCTGTCCTCCATAGAGACTCTTCTCACTAACATACAGGGCCTCTTGAAAGTTGCCATAGACAATGCCAGA GCTCAAGAAAAGCAGGTCCAACTGGAAAAAACAGAGCTGAAGATGGATTTTTTAAGAGAAAGAGAACTAA GAGAAACACTGGAGAAGCAGCTGGCCATGGAACAAAAGAACAGAGCCATAGTTCAAAAGAGGCTAAAGAA GGAAAAGAAAGCAAAGAGAAAACTGCAGGAGGCACTAGAATTTGAGACAAAACGCCGTGAGCAAGCGGAG CAGACACTGAAACAGGCAGCTTCAGCGGACAGTCTCCGGGTCTTAAATGACTCCCTGACCCCTGAGATAG AAGCTGACCGCAGCGGAGGGAGAGCAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTGAAAAC TACTGTCATGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216327 representing NM_007826
Red=Cloning site Green=Tags(s) MAVPAALIPPTQLVPPQPPISTSASSSGTTTSTSSATSSPAPSIGPPASSGPTLFRPEPIASSASSSAAA TVTSPGGGGGGSGGGGGSGGNGGGGGSNCNPSLAAGSSGGGVSAGGGGASSTPITASTGSSSSSSSSSSS SSSSSSSSSSSSSSSSCGPLPGKPVYSTPSPVENTPQNNECKMVDLRGAKVASFTVEGCELICLPQAFDL FLKHLVGGLHTVYTKLKRLEITPVVCNVEQVRILRGLGAIQPGVNRCKLISRKDFETLYNDCTNASSRPG RPPKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTGLTAAAAAAAAATNAAIAEAMKVKKIKLEAMSNYHA SNNQHGADSENGDMNSSVGSSGGSWDKETLHSPPSQGSQAPVAHARMPAAFSLPVSHPLNHLQHSHLPPN GLELPFMMMPHPLIPVSLPPASVTMAMSQMNHLSTIANMAAAAQVQSPPSRVETSVIKERVPDSPSPAPS LEEGRRPGSHPSSHRSSSVSSSPARTESSSDRIPVHQNGLSMNQMLMGLSPNVLPGPKEGDLAGHDMGHE SKRIHIEKDETPLSTPTARDSIDKLSLTGHGQPLPPGFPSPFLFPDGLSSIETLLTNIQGLLKVAIDNAR AQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQKRLKKEKKAKRKLQEALEFETKRREQAE QTLKQAASADSLRVLNDSLTPEIEADRSGGRADAERTIQDGRLYLKTTVMY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007826 |
| ORF Size | 2253 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007826.3, NP_031852.1 |
| RefSeq Size | 5225 bp |
| RefSeq ORF | 2256 bp |
| Locus ID | 13134 |
| UniProt ID | Q9QYB2 |
| MW | 78.4 kDa |
| Gene Summary | Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1 (By similarity). Binds to chromatin DNA via its DACHbox-N domain.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221282 | Dach1 (untagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 1, (10ug) |
CNY 9,690.00 |
|
| MG216327 | Dach1 (tGFP-tagged) - Mouse dachshund 1 (Drosophila) (Dach1) transcript variant 1, (10ug) |
CNY 7,648.00 |
|
| MR216327L3 | Lenti ORF clone of Dach1 (Myc-DDK-tagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 1 |
CNY 9,410.00 |
|
| MR216327L4 | Lenti ORF clone of Dach1 (mGFP-tagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 1 |
CNY 9,410.00 |
