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Gzme (NM_010373) Mouse Tagged ORF Clone
CAT＃: MR216589
- TrueORF®
Gzme (Myc-DDK-tagged) - Mouse granzyme E (Gzme)
ORF Plasmid: tGFP
"NM_010373" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CCP3; Ctla-6; Ctla6; MCSP-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216589 representing NM_010373
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACCAGTCCTGATTCTCCTGACCCTACTTCTGCCTCTTGGAGCTGGAGCAGAGGAGATCATCGGCG GCCATGTGGTGAAGCCACACTCCCGCCCCTACATGGCGTTTGTTAAGTCTGTGGATATTGAAGGTAATAG GAGATACTGTGGAGGCTTCTTGGTTCAAGATGACTTTGTGCTGACTGCTGCTCACTGCAGGAACAGGACA ATGACAGTCACACTGGGGGCCCACAACATCAAGGCTAAGGAGGAGACACAGCAGATCATCCCTGTGGCAA AAGCCATTCCCCATCCAGATTATAATGCCACTGCCTTCTTCAGTGACATCATGCTGTTAAAGCTGGAGAG TAAGGCCAAGAGAACTAAAGCTGTGAGACCCCTCAAGTTGCCCAGACCCAATGCCCGGGTGAAGCCAGGG GATGTGTGCAGTGTGGCTGGCTGGGGGTCAAGGTCCATCAATGACACTAAAGCATCTGCCCGCCTGCGAG AGGCTCAACTGGTCATCCAGGAGGATGAGGAATGCAAAAAACGTTTCCGACACTACACTGAGACCACAGA GATTTGTGCTGGAGACTTGAAGAAAATAAAGACTCCTTTCAAGGGTGACTCTGGGGGACCCCTCGTGTGT GACAACAAAGCTTATGGACTTTTAGCCTATGCAAAAAACAGGACAATCTCTTCAGGAGTCTTCACTAAGA TTGTGCACTTCCTGCCGTGGATAAGCAGGAACATGAAGCTGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216589 representing NM_010373
Red=Cloning site Green=Tags(s) MPPVLILLTLLLPLGAGAEEIIGGHVVKPHSRPYMAFVKSVDIEGNRRYCGGFLVQDDFVLTAAHCRNRT MTVTLGAHNIKAKEETQQIIPVAKAIPHPDYNATAFFSDIMLLKLESKAKRTKAVRPLKLPRPNARVKPG DVCSVAGWGSRSINDTKASARLREAQLVIQEDEECKKRFRHYTETTEICAGDLKKIKTPFKGDSGGPLVC DNKAYGLLAYAKNRTISSGVFTKIVHFLPWISRNMKLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010373 |
| ORF Size | 744 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010373.3, NP_034503.2 |
| RefSeq Size | 923 bp |
| RefSeq ORF | 747 bp |
| Locus ID | 14942 |
| UniProt ID | P08884 |
| MW | 27.5 kDa |
| Gene Summary | This enzyme is probably necessary for target cell lysis in cell-mediated immune responses.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208615 | Gzme (untagged) - Mouse granzyme E (Gzme), (10ug) |
CNY 3,990.00 |
|
| MG216589 | Gzme (tGFP-tagged) - Mouse granzyme E (Gzme), (10ug) |
CNY 2,850.00 |
|
| MR216589L3 | Lenti ORF clone of Gzme (Myc-DDK-tagged) - Mouse granzyme E (Gzme) |
CNY 4,750.00 |
|
| MR216589L4 | Lenti ORF clone of Gzme (mGFP-tagged) - Mouse granzyme E (Gzme) |
CNY 4,750.00 |
