Mrm1 (NM_145433) Mouse Tagged ORF Clone
CAT#: MR217372
- TrueORF®
Mrm1 (Myc-DDK-tagged) - Mouse mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae) (Mrm1), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_145433" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | A530065E19Rik; ENSMUSG00000054212 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR217372 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCGACTCTGGACGGTCGGGTGCTTCAGTCGCCTGATTGCCCGCCATTTCTCCTCGGTGGCTCGGC GCGGGGAACGGCCTGGCGGGGAGGAGCTGAGTCGCTTGCTTCTGGATGACCTGGCGCCGGCCCAGCGGCT GGAACGTCTATTTGGCTTGTCCCCATGCCTCCTGGCCTTGCGGGCTGCCCGTCGGCGCGTGGCCCGGCTC CTGCTCCAGGCCGGTAAGGCTGGGCTGCAGGGGGAGCGAGCCGAGCTGCTCCGGGTGGCCGAGGCTCGGG GCATCCCTGTCTTGAGGCCCAGGCGTCAGAAACTGGATGCCTTGTGCGGCTACCAAGTGCACCAGGGCGT GTGCATGGAGGTGAGCCCGCTGCGACCCCGGCCTTGCGATGAGGCGGCAGACACGAGCTCAGGCGACGAC CCTCAGCAGCTGTGGCTCGTCCTTGAGGGGCTCCAGGATCCCCGCAATCTTGGGGCTGTGATGCGCTCCG CTCACTTCCTCGGCGTAGACAGAGTCATCACCAGCCAGAGAAACAGCTGTCCGCTTACTCCAGTAGTCAG CAAGGCCAGCGCTGGGGCTATGGAGGTGATGGACGTGTTCGCCACTCCTGACCTGCCCGGTTTTTTGCAG GCCAAGGCCCAGCAGGGGTGGCTCGTGGTGGGCACAGTGGGCTGCCCAGGTCCTGAGATCTCCCAGTCCT CCAAGGTCCCCATCACTAGCTGCTTAGAGTTCGTCTGGGACCGGCCTACCCTCCTGGTGCTGGGCAGTGA GGGCTCTGGTCTGTCCCAGGAGGTTTTTGCCTCCTGCCAGCTGCTCCTTACTATTCTGCCCAGACGCCAC CTGCCTCCTGGTCTTGAGTCCTTGAATGTATCTGTGGCCACAGGTATTCTTCTACACTCCATTTGCAGTC AGAAGAAGGGTTTCCCTGTACAGGAGAGAGGACAACTTCTCCAAGACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217372 protein sequence
Red=Cloning site Green=Tags(s) MRRLWTVGCFSRLIARHFSSVARRGERPGGEELSRLLLDDLAPAQRLERLFGLSPCLLALRAARRRVARL LLQAGKAGLQGERAELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVSPLRPRPCDEAADTSSGDD PQQLWLVLEGLQDPRNLGAVMRSAHFLGVDRVITSQRNSCPLTPVVSKASAGAMEVMDVFATPDLPGFLQ AKAQQGWLVVGTVGCPGPEISQSSKVPITSCLEFVWDRPTLLVLGSEGSGLSQEVFASCQLLLTILPRRH LPPGLESLNVSVATGILLHSICSQKKGFPVQERGQLLQDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_145433 |
ORF Size | 963 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145433.1, NP_663408.2 |
RefSeq Size | 2490 bp |
RefSeq ORF | 963 bp |
Locus ID | 217038 |
UniProt ID | Q99J25 |
MW | 34.8 kDa |
Gene Summary | S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1145 (Gm1145) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212659 | Mrm1 (untagged) - Mouse mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae) (Mrm1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,990.00 |
|
MG217372 | Mrm1 (tGFP-tagged) - Mouse mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae) (Mrm1) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,850.00 |
|
MR217372L3 | Lenti ORF clone of Mrm1 (Myc-DDK-tagged) - Mouse mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae) (Mrm1), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
MR217372L4 | Lenti ORF clone of Mrm1 (mGFP-tagged) - Mouse mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae) (Mrm1), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |