Acer1 (NM_175731) Mouse Tagged ORF Clone
CAT#: MR217552
- TrueORF®
Acer1 (Myc-DDK-tagged) - Mouse alkaline ceramidase 1 (Acer1)
ORF Plasmid: tGFP
"NM_175731" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2310024P18Rik; AI662009; Alkcdase1; Asah3; Cer1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR217552 representing NM_175731
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATGTACCGGGCACCAGAGCAAAGATGTCCAGCATCTTTGCCTATCAGAGTTCTGAGGTGGATTGGT GTGAGAGTAATTTCCAGCACTCAGAGTTGGTGGCCGAGTTCTACAATACGTTCAGCAATGTGTTCTTCCT CATCTTTGGACCCCTCATGATGTTCCTCATGCATCCGTATGCCCAGAAGCGTACCCGGTGTTTCTATGGA GTGTCAGTCCTCTTCATGCTCATAGGTCTGTTCTCCATGTATTTCCACATGACACTCAGCTTTCTGGGAC AGCTGCTGGATGAGATCTCCATCCTGTGGTTGTTGGCCAGTGGATACAGTGTGTGGCTGCCCCGTTGCTA TTTTCCCAAGTTCGTCAAGGGGAACAGGTTCTACTTCAGCTGCCTGGTAACTATAACCACTATTATCAGC ACCTTCTTGACGTTCGTGAAGCCCACAGTCAATGCATATGCTCTCAACAGCATCGCCATCCACATCCTCT ACATTGTGCGCACAGAGTACAAGAAGATTAGGGATGATGATCTTCGGCATCTGATTGCGGTTTCTGTGGT CTTATGGGCCGCTGCACTGACCAGCTGGATCAGTGACCGTGTACTTTGCAGCTTCTGGCAGCGGATTCAC TTCTACTACCTGCACAGCATTTGGCACGTCCTCATAAGCATCACATTTCCTTATGGTATCGTGACCATGG CCCTGGTGGATGCAAAGTATGAGATGCCAGATAAAACCCTCAAAGTCCACTACTGGCCCCGGGACAGCTG GGTCATCGGGCTACCCTATGTGGAGATCCAGGAGAATGACAAGAACTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217552 representing NM_175731
Red=Cloning site Green=Tags(s) MHVPGTRAKMSSIFAYQSSEVDWCESNFQHSELVAEFYNTFSNVFFLIFGPLMMFLMHPYAQKRTRCFYG VSVLFMLIGLFSMYFHMTLSFLGQLLDEISILWLLASGYSVWLPRCYFPKFVKGNRFYFSCLVTITTIIS TFLTFVKPTVNAYALNSIAIHILYIVRTEYKKIRDDDLRHLIAVSVVLWAAALTSWISDRVLCSFWQRIH FYYLHSIWHVLISITFPYGIVTMALVDAKYEMPDKTLKVHYWPRDSWVIGLPYVEIQENDKNC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_175731 |
ORF Size | 819 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175731.4, NP_783858.1 |
RefSeq Size | 2429 bp |
RefSeq ORF | 822 bp |
Locus ID | 171168 |
UniProt ID | Q8R4X1 |
MW | 32.5 kDa |
Gene Summary | Endoplasmic reticulum ceramidase that catalyzes the hydrolysis of ceramides into sphingosine and free fatty acids at alkaline pH (PubMed:12783875). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:12783875). Exhibits a strong substrate specificity towards the natural stereoisomer of ceramides with D-erythro-sphingosine as a backbone and has a higher activity towards very long-chain unsaturated fatty acids like the C24:1-ceramide (PubMed:12783875). May also hydrolyze dihydroceramides to produce dihydrosphingosine (By similarity). ACER1 is a skin-specific ceramidase that regulates the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes and more generally plays an important role in skin homeostasis (PubMed:27126290, PubMed:29056331).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212348 | Acer1 (untagged) - Mouse alkaline ceramidase 1 (Acer1), (10ug) |
CNY 3,990.00 |
|
MG217552 | Acer1 (tGFP-tagged) - Mouse alkaline ceramidase 1 (Acer1), (10ug) |
CNY 2,850.00 |
|
MR217552L3 | Lenti ORF clone of Acer1 (Myc-DDK-tagged) - Mouse alkaline ceramidase 1 (Acer1) |
CNY 4,750.00 |
|
MR217552L4 | Lenti ORF clone of Acer1 (mGFP-tagged) - Mouse alkaline ceramidase 1 (Acer1) |
CNY 4,750.00 |