Pla2g2f (NM_012045) Mouse Tagged ORF Clone
CAT#: MR218100
- TrueORF®
Pla2g2f (Myc-DDK-tagged) - Mouse phospholipase A2, group IIF (Pla2g2f)
ORF Plasmid: tGFP
"NM_012045" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GIIFsPLA2; sPLA2-IIF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR218100 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGATGGGGCACAGGCCAACCCCAAAGGGTTCAGGAAGAAGGCGCTGGTTAAACACTCCACTGGAC GGAAGAGCCCAAGCCTCAGGGCCTCTCCCTCTAAAACCTCCAGGTCCAGCCTGGGTATGAAGAAATTCTT TGCCATCGCAGTCCTGGCCGGCAGTGTGGTAACCACGGCCCACAGCAGCCTGCTGAACCTGAAGTCCATG GTGGAGGCCATCACACACAGAAACTCCATCCTGTCCTTTGTGGGCTACGGCTGCTACTGCGGGCTGGGGG GACGCGGCCATCCCATGGATGAGGTAGACTGGTGCTGCCATGCCCACGACTGTTGCTATGAGAAGCTCTT TGAGCAGGGCTGCCGCCCCTACGTGGACCACTATGACCACAGGATCGAGAATGGCACCATGATTGTCTGC ACTGAGCTCAATGAGACGGAGTGTGACAAGCAGACATGCGAGTGTGACAAGAGCCTGACTCTGTGCCTCA AGGATCACCCATACAGGAACAAGTACCGAGGCTACTTCAACGTCTACTGCCAGGGCCCCACACCCAACTG CAGCATCTATGACCCGTACCCAGAGGAAGTCACCTGTGGGCATGGCCTCCCTGCGACCCCTGTCTCAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218100 protein sequence
Red=Cloning site Green=Tags(s) MADGAQANPKGFRKKALVKHSTGRKSPSLRASPSKTSRSSLGMKKFFAIAVLAGSVVTTAHSSLLNLKSM VEAITHRNSILSFVGYGCYCGLGGRGHPMDEVDWCCHAHDCCYEKLFEQGCRPYVDHYDHRIENGTMIVC TELNETECDKQTCECDKSLTLCLKDHPYRNKYRGYFNVYCQGPTPNCSIYDPYPEEVTCGHGLPATPVST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012045 |
ORF Size | 633 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012045.4, NP_036175.2 |
RefSeq Size | 2437 bp |
RefSeq ORF | 633 bp |
Locus ID | 26971 |
UniProt ID | Q9QZT4 |
MW | 23.3 kDa |
Gene Summary | May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases. Phospholipase A2, which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:10531313). Hydrolyzes phosphatidylethanolamine more efficiently than phosphatidylcholine, with only a modest preference for arachidonic acid versus linoelic acid at the sn-2 position. Comparable activity toward 1-palmitoyl-2-oleoyl-phosphatidylserine vesicles to that toward 1-palmitoyl-2-oleoyl-phosphatidylglycerol (PubMed:11877435). Prefers phosphatidylglycerol compared to phosphatidylcholine (PubMed:10531313).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209755 | Pla2g2f (untagged) - Mouse phospholipase A2, group IIF (Pla2g2f), (10ug) |
CNY 3,990.00 |
|
MG218100 | Pla2g2f (tGFP-tagged) - Mouse phospholipase A2 group IIF (Pla2g2f), (10ug) |
CNY 2,850.00 |
|
MR218100L3 | Lenti ORF clone of Pla2g2f (Myc-DDK-tagged) - Mouse phospholipase A2, group IIF (Pla2g2f) |
CNY 4,750.00 |
|
MR218100L4 | Lenti ORF clone of Pla2g2f (mGFP-tagged) - Mouse phospholipase A2, group IIF (Pla2g2f) |
CNY 4,750.00 |
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