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Nek11 (NM_172461) Mouse Tagged ORF Clone
CAT＃: MR218193
- TrueORF®
Nek11 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 11 (Nek11)
ORF Plasmid: tGFP
"NM_172461" in other vectors (4)
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CNY 4,680.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4932416N14Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218193 representing NM_172461
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAAATTCCAAGAGACTGCGAAGTGTGTGGGCAGAAGACCCACAGTCATCCCCATGTACCCGACCG CTTTGATTGCAAGAAGATATGTTCTTCAGCAGAAACTTGGCAGTGGAAGTTTTGGAACTGTCTATCTGGT GTCAGACAAGAAAGCCAAACCTGGAGAGGAGCTAAAGGTACTGAAGGAAATCTCTGTTGGCGAATTAAAT CCAAATGAGACCGTGCAGGCCAATGTGGAAGCTCAGCTCCTCTCCAGGCTCCATCATCCTGCCATTGTCA GATTCCATGCAAGCTTCATGGAGCAGGAGACTTTTTGCATTATCACGGAATACTGTGAGGGCCGAGATCT GGACTATAGAATCCAGGAATATAAAGAAGCTGGGAAAGTCTTCGCTGAAAATCAGATAGTAGAATGGTTT ATCCAGCTGTTGCTTGGAGTTGACTACATGCATGAGAGGAGAATACTTCATCGAGACTTGAAATCAAAGA ATATATTTCTGAAAAATAATCTACTCAAAATCGGGGATTTTGGAGTTTCTCGGCTGCTAATGGGTTCATG TGAGCTGGCTACAACTCTAACCGGGACCCCCCATTATATGAGTCCCGAGGCCCTGAAGCACCAAGGCTAT GATGCCAAGTCTGACATCTGGTCACTGGCATGCATTTTATATGAGATGTGTTGCCTGGATCATGCATTTG CTGGCTCCAGTTTCTTGTCTGTGGTTTTGAATATTGTTGAAGGTAAAACACCTTCACTCCCGGACAGATA TCCACGAGAACTAAACACCATCATGGAACGCATGTTGAACAAGAGTCCTTCATTGAGACCGTCGGCTGCA GACATTTTAAAAGCCCCTTACATGGAAGAGCAGCTTCAGCTCCTGATGTGTAAATACCCAGAGATGACAC TGGAAGACAAGAACTCAGTTTGTCAGAAGGAGGCTGCTCATACGATTAATGCCGTGCAGAAAAAGCTTCA CCTGCAGACTCTGCAAGCCTTGTCTGACACGCAGAAAACGACTCCCAGAGAACGGATGTGGCTGAGGAAG CTGCAGGCAGCCGATGAGAGAGCCAGGAGGCTGAAAAAGATTGCTGAAGAAAACTATAAAGAAAATGACA AAAGGATGCAGGCCCTGAGATCCCGGAATGTGGGGTCCGTGCATGCCCATGTGCTCCATGAACTAGATGA ACGGACTTTGGAGAGCCTGCCTGAGCCTCAGTCCCTTCCGTGCCTGGACCTCGATGAACTTGAGCCCAGT TTAGAGGACACCATTGTTGACCTCGGACATTATGAGATCCCAGAAGACCCACTTGTTGCTGAACAATATT ATTCCGATGTCTTTGATTCCTGTTCTGAAGACAGCGAGGAGCAGGAGGAAGAAATGATATTCTCAGAAGC AGGAGGAGACACGAAAGAGGAGGAATCCCCATCTGTGTACAGAACAAACCAACAGGACAGTGATACTGCC GCGCTGGTTGGGTGTTTGGAACATGTCCTGGGTTACACCTCTCTAGACACAAAGACTATTACCAATGCAG TGACAGACATGTCTCCTGGACCAATGGTTTTCAACAGTGCAGTGGCTCGGACCAAGATGAAGCGCATGAA GGAATCAGCTGTACAGAAGCTAGGAATGGAAACGTTTGAGGAGGTCTACGATTACCTCAAGAGAGCAAGG CACCAGAATGCCAGAGAAGCAGAGATCTGGGAGCATCTGGAGACGGTGGTTCCTCGGGCCAGTGACTGCT TTGAAGTTGACCAGCTCCTCTATTTTGAGGAGTTGCTGCTGACCATGGAAGGAAAAGAGCCCTCTCTCCA GAACCTGCCCTGTGAAGCTGCCCAGAAGAAGCCGGTCAAGGGCACTCATTTCTGTGACAATCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218193 representing NM_172461
Red=Cloning site Green=Tags(s) MLKFQETAKCVGRRPTVIPMYPTALIARRYVLQQKLGSGSFGTVYLVSDKKAKPGEELKVLKEISVGELN PNETVQANVEAQLLSRLHHPAIVRFHASFMEQETFCIITEYCEGRDLDYRIQEYKEAGKVFAENQIVEWF IQLLLGVDYMHERRILHRDLKSKNIFLKNNLLKIGDFGVSRLLMGSCELATTLTGTPHYMSPEALKHQGY DAKSDIWSLACILYEMCCLDHAFAGSSFLSVVLNIVEGKTPSLPDRYPRELNTIMERMLNKSPSLRPSAA DILKAPYMEEQLQLLMCKYPEMTLEDKNSVCQKEAAHTINAVQKKLHLQTLQALSDTQKTTPRERMWLRK LQAADERARRLKKIAEENYKENDKRMQALRSRNVGSVHAHVLHELDERTLESLPEPQSLPCLDLDELEPS LEDTIVDLGHYEIPEDPLVAEQYYSDVFDSCSEDSEEQEEEMIFSEAGGDTKEEESPSVYRTNQQDSDTA ALVGCLEHVLGYTSLDTKTITNAVTDMSPGPMVFNSAVARTKMKRMKESAVQKLGMETFEEVYDYLKRAR HQNAREAEIWEHLETVVPRASDCFEVDQLLYFEELLLTMEGKEPSLQNLPCEAAQKKPVKGTHFCDNP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172461 |
| ORF Size | 1884 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172461.3, NP_766049.2 |
| RefSeq Size | 2625 bp |
| RefSeq ORF | 1887 bp |
| Locus ID | 208583 |
| UniProt ID | Q8C0Q4 |
| MW | 72.1 kDa |
| Gene Summary | Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219858 | Nek11 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 11 (Nek11), (10ug) |
CNY 7,980.00 |
|
| MG218193 | Nek11 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 11 (Nek11), (10ug) |
CNY 5,040.00 |
|
| MR218193L3 | Lenti ORF clone of Nek11 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 11 (Nek11) |
CNY 6,460.00 |
|
| MR218193L4 | Lenti ORF clone of Nek11 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 11 (Nek11) |
CNY 6,460.00 |
