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Prex2 (NM_029525) Mouse Tagged ORF Clone
CAT＃: MR218532
- TrueORF®
Prex2 (Myc-DDK-tagged) - Mouse phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (Prex2), transcript variant 1
ORF Plasmid: tGFP
"NM_029525" in other vectors (4)
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CNY 12,024.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6230420N16Rik; AI316880; AI553603; C030045D06Rik; D430013K02; Depdc2; P-Rex2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218532 representing NM_029525
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGACGAAAGCGCCAGGGAAGTAGACAAGCAGCTTCGCCTGCGCGTGTGCGTGCTCAGCGAGCTGC AGAAGACCGAGCGCGACTACGTGGGCACGCTGGAGTTCCTGGTGTCGGCCTTCTTGCATCGAATGAACCA ATGTGCAGCAGCAAAGGTTGATAAAAATGTGACGGAGGAAACAGTGAAGATGTTATTCTCCAATATCGAG GAGATCCTCATCGTTCATAAGGAGTTCTTGAAAGTTGTAGAAGAATGCTTATATCCGGAACCCAGTGCTC AGCAGGAAGTGGGAGCCTGCTTCCTTCACTTTAAGGACAAGTTTCGTATCTATGATGAATATTGCAGCAA CCATGAAAAGGCACAAAAATTGCTTCTTGAACTGAACAAAATAAGAACAATCCGGACATTTCTTCTGAAC TGCATGCTGCTTGGAGGTCGGAAGAATACAGACGTTCCCCTGGAAGGATATTTAGTGACACCAATTCAGA GGATATGCAAGTACCCTCTTCTTTTGAAGGAATTACTGAAGCGGACTCCACGGAGACATAGTGACTACAC AGCAGTGATGGAAGCACTCCAAGCCATGAAAGCCGTGTGTTCCAACATTAATGAGGCCAAGAGACAGATG GAGAAACTGGAAGTTTTAGAAGAGTGGCAGGCACACATTGAAGGCTGGGAGGGGTCCAACATTACTGACA CCTGTACTGAGATGCTGATGTGTGGAGTCCTAATGAAAATTTCTTCCGGAAATATTCAAGAACGAGTGTT TTTTCTTTTTGATAATCTTTTAGTATACTGCAAAAGAAAACACAGACGGCTGAAGAATAGTAAGGCATCC ACAGATGGGTATCGGTATGTCTTTCGAGGGCGGATCAATACTGAGGTGATGGAAGTGGAAAATGTGGATG ACGGCACAGCTGATTTTCACAGCAGTGGGCATATTGTTGTGAATGGGTGGAAGATACACAACACAGCAAA AAATAAATGGTTTGTATGCATGGCTAAAAGCCCTGAAGAAAAGCATGAGTGGTTTGAAGCTATTTTGAAA GAAAGAGAACGTCGGAAAGGGTTAAAACTAGGAATGGAACAAGACACCTGGGTAATGATCTCTGAGCAGG GTGAGAAATTGTATAAAATGATGTGCAAACAGGGAAATCTGATCAAAGACAGAAAAAGAAAACTGACCAC ATTTCCTAAGTGCTTCCTGGGAAGTGAATTTGTCTCATGGCTATTGGAAATTGGAGAGATTCACAGGCCC GAAGAAGGTGTTCACTTAGGACAAGCGCTGTTAGAGAATGGGATCATCCACCATGTTACCGATAAGCACC AGTTCAAACCGGAGCAAATGCTATACCGATTCCGTTATGACGATGGTACATTTTACCCCAGGAGTGAGAT GCAGGACGTGATTTCTAAGGGTGTAAGGCTCTACTGTCGTCTTCATAGTCTGTTTACCCCAGTGGTCAGA GATAAAGATTACCATTTAAGAACTTACAAATCTGTTGTCATGGCCAACAAATTGATAGACTGGTTAATTG CACAGGGTGATTGCCGTACCAGGGAAGAGGCAATGATATTTGCTGTTGGGCTCTGTGACAATGGATTTAT GCATCACGTCCTAGAAAAAAGTGAATTCAAAGATGAGCCTCTACTTTTCCGCTTTTTTGCTGATGAGGAA ATGGAAGGATCAAATATGAAGCATAGGCTTATGAAACATGACTTGAAGGTTGTGGAGAATGTCATAGCTA AGTCACTGTTGATTAAATCCAACGAAGGCAGCTATGGCTTTGGCCTAGAAGACAAAAACAAAGTTCCAAT AATAAAATTAGTGGAAAAGGGGTCGAATGCCGAGATGGCTGGCATGGAAGTTGGGAAGAAGATATTTGCC ATTAATGGTGACCTTGTTTTTCTGAGACCTTTTCCTGAAGTCGATTGCTTCCTGAAATCGTGCTTAAACA GCAGAAAGCCACTGAGAGTCCTCGTGAGCACAAAGCCAAGAGAGACCGTTAAGATTCCAGATTCTGCAGA CGGGCTTGGTTTCCAGATCAGAGGCTTTGGACCTTCAGTTGTGCATGCGGTGGGAAGAGGTACTGTGGCT GCAGCAGCTGGCCTTCATCCAGGACAGTGTATTATCAAAGTGAATGGAATCAATGTCAGCAAAGAGACAC ATGCCAGTGTCATCGCACATGTCACAGCCTGCCGGAAATACAAACGGCCAATGAAGCAAGATTCCATACA GTGGGTCTACGACAGTCTTGAAAGTGCTCAGGAAGACATTCAGAAGTCCCACTCAAAGCCCCCTGGAGAC GGGGCTGGAGATGCTTTTGAGTGCAAAGTAGAAGATGTCATTGACAAGTTCAACACCATGGCTATTATTG ATGGCAAGAAGGAGCATGTGAGTCTGACGGTGGACAACGTCCACCTCGAGTATGGGGTTGTATATGAGTA TGATAGCACGGCGGGCACAAAGTGCAATGTGGTGGAGAAGATGGTGGAGCCCAAAGGGTTCTTCAGCCTA ACTGCCAAGATTCTGGAAGCCCTAGCAAAAAGTGACGAACATTTTGTCCAAAACTGCACCAGCCTTAATT CTCTGAATGAAGTGATCGCCACTGATCTTCAGAGTAAATTCACCAGCATGTGCAGTGAGAGAATCGAACA TGTGTGTCACAGAATATCCAGCTATGGGAGGTTCTCTCGGGTGCTGAAGAACAGAGCCTGGCCCACCTTT AAGCAGGCCAAACCTAAGATCTCCCCACTGCACAGCAGTGATTTCTGCCCAACCAACTGCCACGTCAATG TGATGGAAGTTTCTTATCCGAAAACATCAACTTCCCTGGGGAGTGCCTTCGGTGTGCAGCTAGATAGCAG AAAACATAATTCTCACGATAAAGAAAATAAATCTGTCGAGCCAGGGAAACTGAGCCCCATGGTGTATATT CAGCACACAATCACAACCATGGCAGCGCCTTCGGGCCTGTCACTGGGACACAAGGATGGGCATGGCCTCC AGTATTTGCTGAAAGAAGAAGACTTAGAAACTCAAGATATCTATCACAAACTTCTGGGCAAGCTGCAGAC TGCACTGAAGGAGGTAGAGATGAGCGTTTGTCAAATTGATGACCTTCTGTCTTCAATAACATATTCTCCT AAATTGGAACGTAAGACAACAGAGTGTGTGACACCAATGGACAGTGACAACGAGAAGGGAGAGAGAAATA GCAAACGTGTCTGTTTTAATGTAGCAGGGGATGAGCAAGAGGATTCAGGCCATGATACAGTCAGCAACAG AGACTCTTACAGTGACTGCAACAGCAACAGGAATTCCATTGCTTCCTTCACGAGTATCTGCAGCAGCCAG TGCAGTTCCTACTTCCACAGTGATGAGATGGACTCAGGTGATGAGCTTCCTATCAGTGTCCGGATATCTC ATGATAAACAAGACAAGATCCACACTTGCCTGGAACAGCTCTTCAGCCAGATTGATTCAATAATCAACCT CTTGAAAGGACAAGCTGTTATAAGAGCCTTTGAGCAGACCAAGTATCTCACACCGGGTCGAGGATTGCAA GAATTCCAACAGGAAATGGAGGCAAAGCTGAGTTGCCCCAGGAGGTTGCGGCTCCATCTCAAGCAGGACC CCTGGAATCTTCCCAGCAGCATCCAGGCGCTTGCACAGAGCATCAGAAAACATGCTGAAGAAGTGAAGTG CCGCATACTACTGGCTCTCCTTGAATACTCAGACAGTGAAACCCAACTCCGGAGAGACATGGTTTTCTGT CAAAGTCTTGTGGCCACTGTCTGTGCCTTCTCTGAGCAGCTCATGGCTGCCTTAAACCAGATGTTTGACA ACAGCAAGGAAAATGAAATGGAGACTTGTGAAGCCAGCAGGAGGTGGCTGGACCAGATTGCAAATGCTGG TGTGCTTTTTCACTTCCAGTCACTTCTGTCACCAAATTTGAAAGATGAACAAGCCATGCTTGAGGATACA CTGGTTGCCCTGTTTGATTTGGAAAAAGTTTCCTTCTTCTTCAAACCATCAGAAGAGGATCCACTGGTTG CAAATGTCCCTCTTACCTACCAAGTGGAAGGAAGCAGGCAGGCTCTGAAAGTTTACTTCTACATGGATAG TTACCATTTTGAGCAGCTGCCCCAACGGTTGAAGAATGGAGGAGGGTTTAAAATTCATCCTGTTCTCTTT TCACAAGCATTGGAGAGTATGGAAGGATATTGCTATAGAGACAACATCTCTGTGGAAGAGTTTCAAGCAC AAATAAACACAGCCTCCCTGGAAAAGGTCAAACAGTACAATCAGAAGCTCAGGGCATTCTACTTGGACAA GTCAAATTCCCCACCGAATACCACATCTAAAGCTGCCTACATAGATAAGCTCATGAAGCCTCTCAATGCT TTAGATGAACTTTACCGGCTGATCACCTCATTTATCAGATCCAAACGCATCGCTGCCTGTGTGAACACAC CTTGCAGCGCCTCTGGAGTCGGACTGCTGTCAGTTTCTTCAGAGCTGTGTGACAGGCTCGGTGCCTGTCA CATCATCATGTGCAGCAGTGGTGTGCACCGGTGCACCCTGAGTGTGACACTAGAGCAAACCATCACTTTA GCAAGAAGCCATGGGCTCCCTCCTCGGTACATTATGCAGGCCATGGATGTGATGAGAAAGCAGGGTGCAA GAGTTCAGAACACAGCTAAAAACTTGGGCGTTAGAGACCGGACTCCTCAGTCAGCTCCAAGGCTGTACAA GCTCTGCGAGCCACCACCTCCAGTAGGAGAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218532 representing NM_029525
Red=Cloning site Green=Tags(s) MSDESAREVDKQLRLRVCVLSELQKTERDYVGTLEFLVSAFLHRMNQCAAAKVDKNVTEETVKMLFSNIE EILIVHKEFLKVVEECLYPEPSAQQEVGACFLHFKDKFRIYDEYCSNHEKAQKLLLELNKIRTIRTFLLN CMLLGGRKNTDVPLEGYLVTPIQRICKYPLLLKELLKRTPRRHSDYTAVMEALQAMKAVCSNINEAKRQM EKLEVLEEWQAHIEGWEGSNITDTCTEMLMCGVLMKISSGNIQERVFFLFDNLLVYCKRKHRRLKNSKAS TDGYRYVFRGRINTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTAKNKWFVCMAKSPEEKHEWFEAILK ERERRKGLKLGMEQDTWVMISEQGEKLYKMMCKQGNLIKDRKRKLTTFPKCFLGSEFVSWLLEIGEIHRP EEGVHLGQALLENGIIHHVTDKHQFKPEQMLYRFRYDDGTFYPRSEMQDVISKGVRLYCRLHSLFTPVVR DKDYHLRTYKSVVMANKLIDWLIAQGDCRTREEAMIFAVGLCDNGFMHHVLEKSEFKDEPLLFRFFADEE MEGSNMKHRLMKHDLKVVENVIAKSLLIKSNEGSYGFGLEDKNKVPIIKLVEKGSNAEMAGMEVGKKIFA INGDLVFLRPFPEVDCFLKSCLNSRKPLRVLVSTKPRETVKIPDSADGLGFQIRGFGPSVVHAVGRGTVA AAAGLHPGQCIIKVNGINVSKETHASVIAHVTACRKYKRPMKQDSIQWVYDSLESAQEDIQKSHSKPPGD GAGDAFECKVEDVIDKFNTMAIIDGKKEHVSLTVDNVHLEYGVVYEYDSTAGTKCNVVEKMVEPKGFFSL TAKILEALAKSDEHFVQNCTSLNSLNEVIATDLQSKFTSMCSERIEHVCHRISSYGRFSRVLKNRAWPTF KQAKPKISPLHSSDFCPTNCHVNVMEVSYPKTSTSLGSAFGVQLDSRKHNSHDKENKSVEPGKLSPMVYI QHTITTMAAPSGLSLGHKDGHGLQYLLKEEDLETQDIYHKLLGKLQTALKEVEMSVCQIDDLLSSITYSP KLERKTTECVTPMDSDNEKGERNSKRVCFNVAGDEQEDSGHDTVSNRDSYSDCNSNRNSIASFTSICSSQ CSSYFHSDEMDSGDELPISVRISHDKQDKIHTCLEQLFSQIDSIINLLKGQAVIRAFEQTKYLTPGRGLQ EFQQEMEAKLSCPRRLRLHLKQDPWNLPSSIQALAQSIRKHAEEVKCRILLALLEYSDSETQLRRDMVFC QSLVATVCAFSEQLMAALNQMFDNSKENEMETCEASRRWLDQIANAGVLFHFQSLLSPNLKDEQAMLEDT LVALFDLEKVSFFFKPSEEDPLVANVPLTYQVEGSRQALKVYFYMDSYHFEQLPQRLKNGGGFKIHPVLF SQALESMEGYCYRDNISVEEFQAQINTASLEKVKQYNQKLRAFYLDKSNSPPNTTSKAAYIDKLMKPLNA LDELYRLITSFIRSKRIAACVNTPCSASGVGLLSVSSELCDRLGACHIIMCSSGVHRCTLSVTLEQTITL ARSHGLPPRYIMQAMDVMRKQGARVQNTAKNLGVRDRTPQSAPRLYKLCEPPPPVGEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029525 |
| ORF Size | 4794 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029525.1, NP_083801.1 |
| RefSeq Size | 11057 bp |
| RefSeq ORF | 4797 bp |
| Locus ID | 109294 |
| UniProt ID | Q3LAC4 |
| MW | 181.7 kDa |
| Gene Summary | Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224738 | Prex2 (untagged) - Mouse phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (Prex2), transcript variant 1, (10ug) |
CNY 20,520.00 |
|
| MG218532 | Prex2 (tGFP-tagged) - Mouse phosphatidylinositol-345-trisphosphate-dependent Rac exchange factor 2 (Prex2) transcript variant 1, (10ug) |
CNY 13,300.00 |
|
| MR218532L3 | Lenti ORF clone of Prex2 (Myc-DDK-tagged) - Mouse phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (Prex2), transcript variant 1 |
CNY 13,970.00 |
|
| MR218532L4 | Lenti ORF clone of Prex2 (mGFP-tagged) - Mouse phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (Prex2), transcript variant 1 |
CNY 13,970.00 |
