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Scin (NM_009132) Mouse Tagged ORF Clone
CAT＃: MR218765
- TrueORF®
Scin (Myc-DDK-tagged) - Mouse scinderin (Scin), transcript variant 2
ORF Plasmid: tGFP
"NM_009132" in other vectors (4)
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CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | adseverin; AW545522 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218765 representing NM_009132
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGGAGCTGCAGCACCCCGAGTTCGCGCGCGCAGGCCAGCAGGCTGGGCTGCAGGTGTGGAGGG TCGAGAAGCTGGAACTGGTACCGGTGCCCCAGGGTGCCTATGGTGACTTTTACGTCGGAGACGCCTACCT GGTGCTGCACACCACCAAGTCCAGCAGGGGCTTCTCCTACCGCCTGCATTTCTGGCTGGGAAAGGAGTGT TCCCAGGATGAGAGCACAGCGGCTGCCATCTTTACGGTCCAGATGGACGACTATTTGGGTGGCAAGCCAG TCCAGAGCAGAGAGCTTCAAGGCTATGAGTCGACTGATTTTGTGGGCTATTTCAAAGGCGGTCTGAAGTA CAAGGCTGGAGGTGTGGCTTCTGGACTAAACCATGTCCTCACCAATGATCTGACTGCGAAAAGACTTCTG CACGTGAAGGGTCGGAGAGTGGTCAGAGCCACTGAAGTTCCCCTCAGCTGGGAGAGCTTCAACAAGGGCG ACTGCTTCATCATTGACCTTGGCACCGAAATTTACCAGTGGTGTGGTTCCTCCTGCAACAAATATGAGCG TCTGAAAGCAAGCCAGGTGGCCATTGGCATCCGGGACAATGAGAGGAAAGGAAGATCTCAACTCATTGTG GTGGAAGAAGGAAGTGAACCATCAGAGCTCATGAAGGTTTTAGGGAGAAAGCCTGAGCTTCCAGATGGGG ACAATGATGACGATGTCGTAGCAGACATAAGTAACAGGAAGATGGCGAAGCTCTACATGGTTTCAGATGC AAGTGGGTCCATGAAAGTAACACTGGTGGCTGAAGAAAACCCGTTCTCCATGGGAATGTTGCTTTCTGAA GAATGCTTCATTTTGGACCATGGTGCTGCAAAACAAATTTTTGTATGGAAAGGTAAAAATGCTAACCCAC AGGAGAGGAAGACTGCCATGAAGACAGCTGAGGAGTTTTTACAGAAAATGAAGTATTCTACTAATACTCA AATTCAGGTTCTTCCGGAAGGCGGTGAAACACCAATTTTCAAACAGTTCTTTAAGGACTGGAAGGATAAA GACCAGAGTGATGGCTTTGGGAAGGTGTACATCACGGAGAAAGTGGCTCAGATAAAGCAGATTCCGTTTG ATGCCTCAAAACTGCACAGTTCTCCGCAGATGGCAGCCCAGCACAACATGGTGGACGATGGCTCTGGCGG GGTGGAGATCTGGCGTGTAGAGAACAGTGGTAGAGTCCAGATTGACCCAAGCTCCTATGGCGAGTTCTAT GGCGGTGACTGCTACATTATCCTCTACACTTATCCCAGAGGACAGATCATCTACACATGGCAAGGAGCAA ATGCTACCAGAGATGAACTGACCATGTCCGCGTTTCTGACTGTCCAGTTGGACCGGTCCCTTGGAGGGCA GGCTGTGCAGGTCCGTGTCTCTCAAGGCAAAGAGCCTGCTCACCTGCTGAGTTTGTTCAAAGACAAACCA CTCATTATTTATAAGAATGGGACATCCAAGAAAGAAGGGCAGGCACCGGCTCCCCCTACACGCCTTTTTC AAGTCCGGAGGAACCTGGCATCTATCACCAGAATTGTGGAGATTGAAGAAGTTCCGGGAGAGTTCACCCA GGATGACCTGGCAGAAGATGATGTCATGCTACTTGATGCGTGGGAACAGATCTTTATTTGGATTGGCAAA GATGCCAATGAAGTTGAGAAAAAGGAATCAGTGAAGTCTGCCAAAATGTACCTGGAGACAGACCCTTCTG GAAGAGACAAGAGGACACCGATTGTCATCATCAAGCAAGGGCACGAGCCCCCCACATTCACAGGCTGGTT TCTGGGCTGGGACTCCAGCAGGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218765 representing NM_009132
Red=Cloning site Green=Tags(s) MAQELQHPEFARAGQQAGLQVWRVEKLELVPVPQGAYGDFYVGDAYLVLHTTKSSRGFSYRLHFWLGKEC SQDESTAAAIFTVQMDDYLGGKPVQSRELQGYESTDFVGYFKGGLKYKAGGVASGLNHVLTNDLTAKRLL HVKGRRVVRATEVPLSWESFNKGDCFIIDLGTEIYQWCGSSCNKYERLKASQVAIGIRDNERKGRSQLIV VEEGSEPSELMKVLGRKPELPDGDNDDDVVADISNRKMAKLYMVSDASGSMKVTLVAEENPFSMGMLLSE ECFILDHGAAKQIFVWKGKNANPQERKTAMKTAEEFLQKMKYSTNTQIQVLPEGGETPIFKQFFKDWKDK DQSDGFGKVYITEKVAQIKQIPFDASKLHSSPQMAAQHNMVDDGSGGVEIWRVENSGRVQIDPSSYGEFY GGDCYIILYTYPRGQIIYTWQGANATRDELTMSAFLTVQLDRSLGGQAVQVRVSQGKEPAHLLSLFKDKP LIIYKNGTSKKEGQAPAPPTRLFQVRRNLASITRIVEIEEVPGEFTQDDLAEDDVMLLDAWEQIFIWIGK DANEVEKKESVKSAKMYLETDPSGRDKRTPIVIIKQGHEPPTFTGWFLGWDSSRW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009132 |
| ORF Size | 1845 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009132.2, NP_033158.2 |
| RefSeq Size | 2708 bp |
| RefSeq ORF | 1848 bp |
| Locus ID | 20259 |
| UniProt ID | Q60604 |
| MW | 69.6 kDa |
| Gene Summary | Ca(2+)-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane (PubMed:9671468). Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate (PIP2) (By similarity). In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+) (PubMed:9671468). Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles (By similarity). Plays a role in osteoclastogenesis (OCG) and actin cytoskeletal organization in osteoclasts (PubMed:25275604, PubMed:25681458). Regulates chondrocyte proliferation and differentiation (By similarity). Inhibits cell proliferation and tumorigenesis. Signaling is mediated by MAPK, p38 and JNK pathways (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219710 | Scin (untagged) - Mouse scinderin (Scin), transcript variant 2, (10ug) |
CNY 7,890.00 |
|
| MG218765 | Scin (tGFP-tagged) - Mouse scinderin (Scin) transcript variant 2, (10ug) |
CNY 5,040.00 |
|
| MR218765L3 | Lenti ORF clone of Scin (Myc-DDK-tagged) - Mouse scinderin (Scin), transcript variant 2 |
CNY 6,460.00 |
|
| MR218765L4 | Lenti ORF clone of Scin (mGFP-tagged) - Mouse scinderin (Scin), transcript variant 2 |
CNY 6,460.00 |
