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Net1 (NM_019671) Mouse Tagged ORF Clone
CAT＃: MR218791
- TrueORF®
Net1 (Myc-DDK-tagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 1
ORF Plasmid: tGFP
"NM_019671" in other vectors (4)
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CNY 5,890.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mNET1; Net1a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218791 representing NM_019671
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCAGAGCCGGCGGCTCAGAAGCAGCCCCGACCTCGGAGGAGAAGCCGCAGGGTCTCAATGCTCA GCGAGGAACCTGCCGCGGGGCTTCCGGCCGACACACCCGGGCCGGCGGCGAACGAGAGATGCTCCCTTCG GAGAGGCAGCTCCTTCACATTTTTAACCCCTGGCCCACACTGGGACTTCACTTTGAAAAGAAAACGAAGA GAGAAAGATGATGATGCTGTAAGCCTTAGCAGCCTTGATTTGAAGGAGCCAAGCAATAAGAGAGTTCGGC CTCTAGCTCGGGTCACATCTTTGGCAAACTTAATCTCTCCCGTAAGAAATGGAGCAGTCAGGCGCTTCGG TCAGACCATACAGTCGTTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTCATTCAGC AGGTCCACCGTCCCCACACCCACCAAGAGAAGAAGCAGTGCACTATGGTCGGAAATGCTAGACATCAATA TGAAGGAGTCCTTGACCACTCGAGAGATCAAACGTCAGGAGGCCATATATGAATTGTCCCGAGGGGAACA GGACTTGATTGAGGATCTCAAGCTCGCTAGAAAGGCCTACCATGACCCCATGTTAAAGCTGTCTATCATG TCAGAAGAGGAACTCACGCACATATTTGGAGACTTGGATGCTTATATACCTCTGCACGAAGATCTGTTAG CAAGAATTGGAGAAGCAACCAAGCCTGATGGGACTGTGGAGCAGATCGGTCACATTCTCGTGAACTGGTT ACCAGGCCTGAATGCCTACAGAGGCTACTGCAGTAACCAGCTGGCGGCCAAGGCTCTTCTGGATCAGAAG AAACAAGACCCAAGAGTCCAAGACTTCCTCCAGCGCTGTCTGGAGTCTCCTTTCAGTCGAAAACTAGATC TTTGGAGTTTCCTAGATATCCCTCGAAGTCGCCTCGTCAAGTACCCCTTACTGTTAAAAGAAATCCTTAG ACACACTCCCAAAGACCACCGTGATGTTCAGCTTCTAGAAGAGGCAATATTGATAATACAAGGAGTTCTT TCTGATATCAACTTGAAGAAAGGCGAATCTGAGTGTCAGTATTACATCAACAAGCTGGAGTACCTGGACG AGAAGCAGAAGGACCCTAGGATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGAAGAATAAAAG CGGCCATAAACTGTACATTTTCCTATTTCAAGACATCTTGGTTTTGACTCGGCCTGTCACCCGAAATGAG CGCCACTTGTACCAGGTTTACCGGCAGCCCATCCCAGTCCAGGAGCTGGTGCTGGAAGACCTGCAGGACG GGGATGTGCGCATGGGTGGCTCCTTCCGAGGGGCCTTCGGCAACTCAGACAAAGCTAAAAATATCTTTAG GGTCCGCTTCCAAGACCCTTCTCCAGGCCACTCCCACACACTGCAAGCCAATGATGTGTTCCATAAGCAG CAGTGGTTCAACTGTATTCGCGCGGCCATTGCCCCCTTCCAGCGGGCCGCCAGCCCCTTAGAGCTTCAGG GCTTGCCAGACCTCCACGAAGAGTGTGAAGAGAACAACCCTTCTGCTGGGAACCTCAGAGCCCAGCGACG GTCATGCGTGGTTCCTGGTGTCATGCAGATAGACGAGGAAAGCGCCCTGGATTGTGGCTCCAGTGTACAG ACAGTGGAAGACACCAGGAACATGAAGGCACAGCGGCCTCAGCCTGGCTTACGAAGAGCAAGGGACAAAG CACAGTCAGGTGGCAAAAAGAAAGAGACTTTGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218791 representing NM_019671
Red=Cloning site Green=Tags(s) MEPEPAAQKQPRPRRRSRRVSMLSEEPAAGLPADTPGPAANERCSLRRGSSFTFLTPGPHWDFTLKRKRR EKDDDAVSLSSLDLKEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKSFS RSTVPTPTKRRSSALWSEMLDINMKESLTTREIKRQEAIYELSRGEQDLIEDLKLARKAYHDPMLKLSIM SEEELTHIFGDLDAYIPLHEDLLARIGEATKPDGTVEQIGHILVNWLPGLNAYRGYCSNQLAAKALLDQK KQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILRHTPKDHRDVQLLEEAILIIQGVL SDINLKKGESECQYYINKLEYLDEKQKDPRIEASKVLLCHGELKNKSGHKLYIFLFQDILVLTRPVTRNE RHLYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFGNSDKAKNIFRVRFQDPSPGHSHTLQANDVFHKQ QWFNCIRAAIAPFQRAASPLELQGLPDLHEECEENNPSAGNLRAQRRSCVVPGVMQIDEESALDCGSSVQ TVEDTRNMKAQRPQPGLRRARDKAQSGGKKKETLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019671 |
| ORF Size | 1785 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019671.3, NP_062645.2 |
| RefSeq Size | 3465 bp |
| RefSeq ORF | 1788 bp |
| Locus ID | 56349 |
| UniProt ID | Q9Z206 |
| MW | 68.2 kDa |
| Gene Summary | Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway. Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219526 | Net1 (untagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 1, (10ug) |
CNY 7,700.00 |
|
| MG218791 | Net1 (tGFP-tagged) - Mouse neuroepithelial cell transforming gene 1 (Net1) transcript variant 1, (10ug) |
CNY 6,460.00 |
|
| MR218791L3 | Lenti ORF clone of Net1 (Myc-DDK-tagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 1 |
CNY 7,790.00 |
|
| MR218791L4 | Lenti ORF clone of Net1 (mGFP-tagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 1 |
CNY 7,790.00 |
