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Pck2 (NM_028994) Mouse Tagged ORF Clone
CAT＃: MR218939
- TrueORF®
Pck2 (Myc-DDK-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (Pck2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_028994" in other vectors (3)
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CNY 6,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1810010O14Rik; 9130022B02Rik; PEPCK-M |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218939 representing NM_028994
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCTTCCTTCCCCGCCTTCCTTGTCTCCCCGACTGCGCTCGTTCCTGTGGCCATCCCGCAGCGCCA GTCCAGGTGCCATGGCTGCTATGTACCTCCCCGGCCTGCGGCTTAGCCGGCACGGGCTAAGGCCCTGGTG CTGGTCACCGTGCCGTAGCATCCAAACCCTGCATGTGCTCAGTGGAGATATGAGTCAGCTGCCGGCTGGA GTTCGAGACTTTGTGGCGCGCAGTGCGCATCTGTGCCAACCAGAGGGCATCCACATCTGTGATGGGACCG AGGCTGAGAACACTGCCATACTGGCCCTGCTGGAAGAACAGGGTCTTATCCGCAAACTCCCCAAGTATAA GAACTGCTGGCTGGCCCGCACAGACCCCAAGGATGTGGCACGGGTAGAAAGCAAGACGGTGATTGTAACT CCTTCGCAGCGGGACACAGTGCCTCTCCTGGCTGGTGGGGCCAGGGGGCAGCTGGGCAACTGGATGTCCC CAGATGAGTTCCAGAGAGCTGTGGACGAGAGATTCCCAGGATGCATGCAGGGCCGCATTATGTATGTGCT TCCATTCAGCATGGGTCCCGTGGGCTCCCCACTCTCCCGCATTGGAGTGCAGCTCACTGACTCGGCTTAC GTGGTGGCCAGTATGCGTATTATGACCCGCCTGGGGACACCTGTACTTCAGGCCCTGGGAGATGGTGACT TCATCAAGTGTCTGCATTCAGTGGGCCAGCCCCTGACTGGACATGGGGATCCTGTGGGCCAGTGGCCGTG CAATCCAGAAAAAACCCTGATTGGCCACGTGCCAGACCAGCGGGAGATCGTCTCCTTCGGCAGCGGCTAT GGTGGTAACTCCTTGCTGGGCAAGAAGTGCTTTGCCCTGCGCATCGCCTCTCGCCTGGCCAGGGATGAGG GCTGGCTGGCAGAGCACATGCTGATTTTGGGCATCACCAACCCTGCAGGGAAAAAGCGCTACGTGGCAGC TGCTTTCCCCAGTGCCTGTGGCAAGACCAATCTGGCCATGATGAGGCCTGCATTGCCGGGCTGGAAAGTG GAGTGTGTGGGGGATGACATCGCCTGGATGAGGTTTGACAGTGAAGGTCAACTCCGGGCCATCAACCCTG AGAATGGCTTCTTTGGGGTGGCCCCTGGTACCTCTGCTGCCACCAATCCCAATGCCATGGCCACAATTCA GAGTAATACTCTCTTCACCAACGTGGCTGAGACCAGTGATGGCGGTGTGTACTGGGAAGGCATTGACCAG CCTCTTCCGCCTGGTGTCACCATAACCTCGTGGCTGGGAAAGCCCTGGAAACCTGGTGACAAGGAACCCT GTGCACATCCAAACTCGCGCTTTTGTGTCCCGGCTCGCCAGTGCCCCATCATGGACCCAGCCTGGGAAGC ACCAGAAGGTGTCCCTATTGATGCCATCATCTTTGGAGGCCGCCGACCCAAAGGGGTACCACTGGTGTAC GAGGCCTTCAACTGGCGTCATGGGGTGTTTGTAGGTAGCGCCATGCGCTCTGAGTCCACTGCCGCTGCGG AGCACAAAGGAAAGACCATTATGCATGATCCCTTTGCCATGCGGCCTTTTTTTGGCTATAACTTTGGACG CTACCTGGAACACTGGTTGAGCATGGAGGGACAAAAAGGTGCCCGGCTGCCTCGTATCTTCCATGTCAAT TGGTTCCGGAGAGATGAAGCAGGCCGCTTCCTGTGGCCAGGCTTTGGAGAGAATGCTCGTGTGCTAGACT GGATCTGCCGAAGATTAGAAGGAGAAGACAGTGCCCAAGAGACTCCCATTGGGCTAGTACCAAAGGAAGG AGCCCTGGACCTCAGTGGCCTCAGCGCAGTGGACACCAGTCAGCTGTTCTCCATCCCCAAGGACTTCTGG GAGCAGGAGGTTCGTGATATTCGGGGCTACCTGACAGAGCAAGTCAACCAGGACCTGCCCAAGGAGGTGT TGGCTGAGCTCGAGGCCCTGGAAGGACGTGTACAAAAAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218939 representing NM_028994
Red=Cloning site Green=Tags(s) MLLPSPPSLSPRLRSFLWPSRSASPGAMAAMYLPGLRLSRHGLRPWCWSPCRSIQTLHVLSGDMSQLPAG VRDFVARSAHLCQPEGIHICDGTEAENTAILALLEEQGLIRKLPKYKNCWLARTDPKDVARVESKTVIVT PSQRDTVPLLAGGARGQLGNWMSPDEFQRAVDERFPGCMQGRIMYVLPFSMGPVGSPLSRIGVQLTDSAY VVASMRIMTRLGTPVLQALGDGDFIKCLHSVGQPLTGHGDPVGQWPCNPEKTLIGHVPDQREIVSFGSGY GGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITNPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKV ECVGDDIAWMRFDSEGQLRAINPENGFFGVAPGTSAATNPNAMATIQSNTLFTNVAETSDGGVYWEGIDQ PLPPGVTITSWLGKPWKPGDKEPCAHPNSRFCVPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVY EAFNWRHGVFVGSAMRSESTAAAEHKGKTIMHDPFAMRPFFGYNFGRYLEHWLSMEGQKGARLPRIFHVN WFRRDEAGRFLWPGFGENARVLDWICRRLEGEDSAQETPIGLVPKEGALDLSGLSAVDTSQLFSIPKDFW EQEVRDIRGYLTEQVNQDLPKEVLAELEALEGRVQKM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028994 |
| ORF Size | 2001 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028994.2, NP_083270.1 |
| RefSeq Size | 3400 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 74551 |
| UniProt ID | Q8BH04 |
| MW | 73.9 kDa |
| Gene Summary | Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218939 | Pck2 (tGFP-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (Pck2) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 7,320.00 |
|
| MR218939L3 | Lenti ORF clone of Pck2 (Myc-DDK-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (Pck2), nuclear gene encoding mitochondrial protein |
CNY 8,550.00 |
|
| MR218939L4 | Lenti ORF clone of Pck2 (mGFP-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (Pck2), nuclear gene encoding mitochondrial protein |
CNY 8,550.00 |
