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Kcnh1 (NM_010600) Mouse Tagged ORF Clone
CAT＃: MR219363
- TrueORF®
Kcnh1 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, subfamily H (eag-related), member 1 (Kcnh1), transcript variant 1
ORF Plasmid: tGFP
"NM_010600" in other vectors (4)
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CNY 7,248.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EAG1; Kv10.1; M-eag |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219363 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGGCTGGCGGCCGGCGGGGACTAGTGGCCCCGCAGAACACGTTTCTGGAGAACATCGTGCGGC GGTCCAACGACACTAATTTTGTGTTGGGGAATGCCCAGATAGTCGACTGGCCCATCGTGTACAGCAATGA TGGATTCTGCAAGCTGTCTGGCTACCACCGGGCAGAAGTGATGCAGAAAAGCAGCGCCTGCAGTTTTATG TATGGAGAGCTGACTGACAAGGACACAGTTGAAAAGGTTCGCCAGACCTTTGAGAACTACGAGATGAATT CCTTCGAAATTCTGATGTACAAGAAGAACAGGACACCTGTGTGGTTTTTTGTGAAGATCGCTCCGATCAG GAACGAACAGGATAAAGTGGTCCTGTTCCTTTGCACTTTCAGCGACATAACTGCATTCAAGCAGCCCATC GAGGACGACTCCTGCAAAGGTTGGGGGAAGTTTGCCCGACTGACCAGAGCTCTGACAAGCAGCAGGGGAG TCCTGCAGCAGCTGGCCCCCAGTGTACAGAAGGGTGAGAATGTTCACAAGCACTCGCGCCTGGCAGAGGT CCTGCAGCTGGGTTCAGACATCCTTCCCCAGTACAAGCAAGAGGCGCCAAAGACACCCCCTCACATCATC CTACACTACTGTGTCTTTAAGACCACGTGGGATTGGATCATCTTGATCCTGACCTTCTACACAGCCATCT TGGTCCCTTACAACGTCTCCTTTAAAACCAGGCAGAATAATGTGGCCTGGCTGGTGGTGGACAGCATCGT GGATGTCATCTTTTTGGTGGACATTGTTTTGAATTTTCACACCACCTTTGTCGGGCCAGCGGGGGAAGTG ATATCTGACCCCAAACTTATCCGCATGAACTACCTGAAGACGTGGTTTGTGATCGACCTTCTGTCCTGTT TGCCGTATGACGTCATCAACGCTTTTGAGAACGTGGATGAGGTTAGTGCCTTTATGGGTGATCCAGGGAA GATTGGTTTTGCTGATCAGATCCCACCACCACTGGAGGGGAGAGAGAGTCAGGGCATCAGCAGCCTGTTC AGTTCTCTGAAAGTTGTGCGGCTGCTCCGTCTCGGGCGAGTGGCCCGCAAACTGGATCATTACATCGAGT ATGGAGCCGCGGTACTGGTCCTGTTGGTGTGCGTGTTTGGGCTTGCTGCCCACTGGATGGCCTGCATCTG GTACAGCATTGGGGACTATGAGATCTTTGATGAAGACACCAAGACCATCCGCAACAACAGCTGGCTCTAC CAACTGGCATTGGACATCGGCACTCCATACCAGTTTAATGGGTCTGGTTCAGGGAAGTGGGAAGGTGGGC CAAGCAAGAACTCTGTCTACATCTCCTCGCTGTACTTCACCATGACAAGTCTCACCAGTGTGGGCTTTGG TAACATTGCCCCATCCACAGACATCGAGAAGATCTTCGCAGTGGCCATCATGATGATTGGCTCTCTTCTG TATGCCACCATCTTTGGGAACGTGACAACCATTTTCCAGCAGATGTACGCCAACACCAACAGGTACCACG AGATGCTCAACAGTGTCCGGGATTTCCTGAAGCTCTACCAGGTGCCCAAAGGGCTGAGTGAGCGGGTCAT GGACTACATTGTGTCCACCTGGTCCATGTCCCGAGGCATCGACACAGAGAAGGTCCTGCAGATCTGCCCC AAGGACATGCGAGCCGACATTTGCGTGCACCTGAACCGCAAAGTGTTCAAAGAACACCCTGCCTTTCGAT TGGCCAGCGATGGCTGCCTGCGGGCCTTGGCCATGGAGTTCCAGACAGTGCACTGTGCCCCAGGGGACCT CATCTATCATGCTGGAGAGAGTGTGGACAGCCTCTGCTTCGTGGTCTCAGGCTCCCTGGAGGTGATCCAG GATGACGAGGTGGTGGCCATCCTAGGGAAAGGAGACGTGTTTGGGGATGTTTTCTGGAAGGAAGCTACCC TTGCACAGTCCTGTGCTAATGTCCGGGCCTTGACCTACTGTGACCTGCACGTGATCAAGAGGGACGCCCT TCAGAAAGTGCTAGAATTCTACACAGCCTTCTCCCACTCCTTCTCCCGGAACCTGATTCTCACCTACAAC CTGAGGAAGAGGATTGTGTTCCGGAAGATCAGCGACGTGAAACGAGAAGAGGAGGAGAGGATGAAACGGA AGAACGAGGCCCCCCTTATCCTGCCTCCTGACCACCCCGTCAGAAGACTCTTCCAAAGGTTCCGCCAGCA GAAAGAAGCCAGGCTGGCAGCCGAGAGAGGGGGCCGGGACCTGGATGACCTGGATGTAGAAAAGGGCAAT GCCCTCACGGACCATACCTCAGCCAACCACAGCCTGGTGAAGGCCAGTGTGGTCACGGTGCGTGAGAGCC CCGCCACGCCTGTGTCCTTCCAGGCGGCCACCACCTCCACCGTGTCAGACCACGCCAAGCTGCATGCCCC CGGATCTGAGTGCCTAGGTCCCAAGGCAGTCAGCTGTGACCCTGCCAAGCGCAAAGGCTGGGCCCGTTTC AAAGATGCCTGTGGAAAGGGTGAGGATTGGAACAAGGTGTCCAAGGCAGAGTCCATGGAGACGCTGCCCG AGAGGACAAAGGCACCGGGTGAGGCCACGCTGAAGAAGACAGACTCCTGTGACAGCGGAATCACCAAGAG CGACCTGCGCTTGGACAATGTGGGTGAGACCAGGAGTCCTCAGGACCGGAGCCCCATCTTGGCTGAGGTC AAGCATTCTTTCTACCCCATCCCTGAGCAGACGCTGCAGGCCACAGTGCTGGAGGTGAAATATGAGCTGA AGGAGGATATCAAGGCCTTGAATGCCAAAATGACCTCCATTGAGAAGCAGCTGTCTGAGATACTCAGGAT ACTAATGTCCAGAGGGTCCGCCCAATCTCCTCAGGAGACGGGTGAGATCTCCAGGCCCCAGTCCCCAGAG TCAGACAGAGACATTTTTGGAGCAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219363 protein sequence
Red=Cloning site Green=Tags(s) MTMAGGRRGLVAPQNTFLENIVRRSNDTNFVLGNAQIVDWPIVYSNDGFCKLSGYHRAEVMQKSSACSFM YGELTDKDTVEKVRQTFENYEMNSFEILMYKKNRTPVWFFVKIAPIRNEQDKVVLFLCTFSDITAFKQPI EDDSCKGWGKFARLTRALTSSRGVLQQLAPSVQKGENVHKHSRLAEVLQLGSDILPQYKQEAPKTPPHII LHYCVFKTTWDWIILILTFYTAILVPYNVSFKTRQNNVAWLVVDSIVDVIFLVDIVLNFHTTFVGPAGEV ISDPKLIRMNYLKTWFVIDLLSCLPYDVINAFENVDEVSAFMGDPGKIGFADQIPPPLEGRESQGISSLF SSLKVVRLLRLGRVARKLDHYIEYGAAVLVLLVCVFGLAAHWMACIWYSIGDYEIFDEDTKTIRNNSWLY QLALDIGTPYQFNGSGSGKWEGGPSKNSVYISSLYFTMTSLTSVGFGNIAPSTDIEKIFAVAIMMIGSLL YATIFGNVTTIFQQMYANTNRYHEMLNSVRDFLKLYQVPKGLSERVMDYIVSTWSMSRGIDTEKVLQICP KDMRADICVHLNRKVFKEHPAFRLASDGCLRALAMEFQTVHCAPGDLIYHAGESVDSLCFVVSGSLEVIQ DDEVVAILGKGDVFGDVFWKEATLAQSCANVRALTYCDLHVIKRDALQKVLEFYTAFSHSFSRNLILTYN LRKRIVFRKISDVKREEEERMKRKNEAPLILPPDHPVRRLFQRFRQQKEARLAAERGGRDLDDLDVEKGN ALTDHTSANHSLVKASVVTVRESPATPVSFQAATTSTVSDHAKLHAPGSECLGPKAVSCDPAKRKGWARF KDACGKGEDWNKVSKAESMETLPERTKAPGEATLKKTDSCDSGITKSDLRLDNVGETRSPQDRSPILAEV KHSFYPIPEQTLQATVLEVKYELKEDIKALNAKMTSIEKQLSEILRILMSRGSAQSPQETGEISRPQSPE SDRDIFGAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010600 |
| ORF Size | 2970 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010600.1, NM_010600.2, NM_010600.3, NP_034730.1 |
| RefSeq Size | 7269 bp |
| RefSeq ORF | 2970 bp |
| Locus ID | 16510 |
| UniProt ID | Q60603 |
| MW | 111.3 kDa |
| Gene Summary | Pore-forming (alpha) subunit of a voltage-gated delayed rectifier potassium channel (PubMed:19671703, PubMed:23975098). Channel properties are modulated by subunit assembly. Mediates IK(NI) current in myoblasts. Involved in the regulation of cell proliferation and differentiation, in particular adipogenic and osteogenic differentiation in bone marrow-derived mesenchymal stem cells (MSCs) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222991 | Kcnh1 (untagged) - Mouse potassium voltage-gated channel, subfamily H (eag-related), member 1 (Kcnh1), transcript variant 1, (10ug) |
CNY 12,830.00 |
|
| MG219363 | Kcnh1 (tGFP-tagged) - Mouse potassium voltage-gated channel subfamily H (eag-related) member 1 (Kcnh1) transcript variant 1, (10ug) |
CNY 7,890.00 |
|
| MR219363L3 | Lenti ORF clone of Kcnh1 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, subfamily H (eag-related), member 1 (Kcnh1), transcript variant 1 |
CNY 9,030.00 |
|
| MR219363L4 | Lenti ORF clone of Kcnh1 (mGFP-tagged) - Mouse potassium voltage-gated channel, subfamily H (eag-related), member 1 (Kcnh1), transcript variant 1 |
CNY 9,030.00 |
