Chst11 (NM_021439) Mouse Tagged ORF Clone

CAT#: MR219389

  • TrueORF®

Chst11 (Myc-DDK-tagged) - Mouse carbohydrate sulfotransferase 11 (Chst11)

ORF Plasmid: DDK tGFP



  "NM_021439" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1110020P09Rik; C4s; C4ST; C4ST-1; C4ST1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR219389 representing NM_021439
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCGGCGCTGCTGGAAGTGATGAGGATGAACAGAATTTGCCGGATGGTGCTGGCCACTTGCTTCG
GATCCTTTATCTTGGTCATCTTCTATTTCCAAAGTATGTTGCACCCAGTCATGCGGAGGAACCCCTTCGG
TGTGGACATCTGCTGCCGGAAGGGATCGAGAAGTCCCCTGCAGGAGCTCTACAATCCCATCCAGCTGGAG
CTATCCAACACTGCCATCCTGCACCAGATGAGACGGGACCAGGTGACAGACACCTGCCGGGCCAACAGCG
CCATGAGCCGCAAGCGCAGGGTGCTGACCCCCAACGACCTGAAGCACCTGGTGGTGGATGAGGACCACGA
ACTCATCTACTGCTATGTGCCCAAGGTAGCGTGCACCAACTGGAAGAGGCTCATGATGGTCCTGAGTGGC
CGGGGCAAGTACAGCGATCCCATGGAGATCCCAGCCAACGAAGCCCACGTGTCGGCCAACCTGAAGACCC
TTAACCAGTACAGCATCCCAGAGATCAACCACCGCTTGAAAAGCTACATGAAGTTCCTGTTCGTGCGGGA
ACCCTTCGAGAGGCTGGTGTCTGCCTACCGCAACAAGTTCACGCAGAAGTACAACACCTCCTTCCACAAG
CGCTACGGCACCAAGATCATCCGACGCCAGCGGAAGAACGCCACGCAGGAGGCCCTGCGCAAGGGGGACG
ATGTCAAGTTCGAGGAGTTCGTGGCCTACCTCATCGACCCCCACACCCAGCGGGAGGAGCCCTTCAACGA
GCACTGGCAGACGGTCTACTCTCTCTGCCACCCGTGCCACATCCACTACGACCTCGTGGGCAAGTATGAG
ACACTGGAGGAGGACTCCAATTACGTACTGCAGCTGGCCGGAGTGAGCGGCTACCTGAAGTTCCCCACCT
ATGCAAAGTCCACCCGAACTACCGACGAGATGACCACGGAGTTCTTCCAGAACATCAGCGCCGAGCACCA
GACACAGCTGTATGAAGTCTACAAACTGGACTTTTTAATGTTCAACTACTCAGTGCCAAACTACCTGAAG
TTGGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR219389 representing NM_021439
Red=Cloning site Green=Tags(s)

MKPALLEVMRMNRICRMVLATCFGSFILVIFYFQSMLHPVMRRNPFGVDICCRKGSRSPLQELYNPIQLE
LSNTAILHQMRRDQVTDTCRANSAMSRKRRVLTPNDLKHLVVDEDHELIYCYVPKVACTNWKRLMMVLSG
RGKYSDPMEIPANEAHVSANLKTLNQYSIPEINHRLKSYMKFLFVREPFERLVSAYRNKFTQKYNTSFHK
RYGTKIIRRQRKNATQEALRKGDDVKFEEFVAYLIDPHTQREEPFNEHWQTVYSLCHPCHIHYDLVGKYE
TLEEDSNYVLQLAGVSGYLKFPTYAKSTRTTDEMTTEFFQNISAEHQTQLYEVYKLDFLMFNYSVPNYLK
LD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021439
ORF Size 1056 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021439.1, NM_021439.2, NP_067414.2
RefSeq Size 5532 bp
RefSeq ORF 1059 bp
Locus ID 58250
UniProt ID Q9JME2
MW 42.1 kDa
Gene Summary Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues in desulfated dermatan sulfate. Preferentially sulfates in GlcA->GalNAc unit than in IdoA->GalNAc unit. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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