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Ano10 (NM_133979) Mouse Tagged ORF Clone
CAT＃: MR219834
- TrueORF®
Ano10 (Myc-DDK-tagged) - Mouse anoctamin 10 (Ano10). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_133979" in other vectors (4)
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CNY 5,400.00
CNY 6,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI604832; Tmem16k |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219834 representing NM_133979, codon optimized.
Due to the complexity of NM_133979, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGTGACGTTGAGCACCCTGGATACCTGCGAGTCTTCCTTTACACCCTTGGTGGTAATCGAACTGG CTCAGGACGTGAAGGATGAGACAAAAGAATGGCTGAAAAATCGCATCATTGCAAAGAAGAAAGACGGGGG AGCACAGTTGCTGTTCCGCCCACTGCTGAATAAGTATGAAAAGGAGACCCTCGAGAATCAGAACCTCTAC CTCGTGGGTGCTAGCAATGTGCGGCTCCTTCTTGGCGCTGAGGCAGTGGGACTGGTGAAAGAGTGTACTG ATGCCGCTATGCGAGCATTTACTTATGGCACAAGACACAACTTTAAGGGCTTTCACGACAACAATAATGA TTTTCTGACAATGGCCGAGTGCCAGTTCATCATCAAGCACGAGCTGGAGAACCTTAGAGCTCGAGATGAG AAGATGATCCCTGGGTATCCTCAGGCTAAACTGTACCCAGGAAAATCTCTGATGCGGCGATTGCTGACTA GTGGAATTGTCACCCAGGTGTTTCCACTCCACGACACTGAAGCTCTCAAAAAGTTGGAGGACACCTGGTA TACCCGGTTCGCATTGAAGTATCAGCCCATCGATAGCATCAGAAGCTATTTTGGCGAAACCATTGCACTC TATTTTGGCTTTTTGGAGTATTTCACATTTGCACTGATCCCTATGGCGATAATTGGACTCCCCTACTACC TCTTTGTGTGGGAAGATTATGATAAGTATGTTATCTTTGCCAGCTTCAATCTCATTTGGTCAACTGTAAT CCTCGAGGTGTGGAAGCGGGGATGCGCTAACATGACTTATCGATGGGGGACGCTGGTGATGAAGCGCCAG TTTGAAGAGCCACGCCCTGGTTTTCATGGAGTTCTGGGCATTAACAGTGTCACAGGGAGGGAAGAGCCAC TCTATAGTTCTTACAAGAGACAGCTGAGAATCTATCTGGTGAGCCTGCCCTTTGTGTGCCTGTGCCTGTA TTTTAGTCTGTATGTGATGATGATATATTTCGACATGGAAGACTGGGCACTGTCCTTGCATGAAGACTCA GGTTCAGAATGGACAAGCCTGTTGCTGTACGTTCCAAGTATCGTCTACGCAGTGGTGATAGAGATCATGA ATAGACTGTATCGGTACGCCGCAGAGTTCCTTACCAGCTGGGAGAACCACCGGTTGGAGTCCGCTTACCA GAACCACCTGGTGCTGAAAGTCCTGGTTTTCAACTTTTTGAACTGCTTTGCCAGCCTCTTTTATATAGCT TTTGTGCTGAAAGATATGAAACTGTTGAGACAGTCTCTGGCAACATTGCTGATCACCTCACAGATACTGA ATCAAGTGGTGGAATCACTGCTTCCCTATTGGCTGCAACGGAAGTACTGCGCACGGGTTAAAAGGAAAGT GCAGGCTCTCAAAAGCGAAGTGGATACCACACTGTATGAGCAGGTGCTTTTGGAGAAGGAGATGGGCACA TACTTGGGGACATTCGATGATTACTTGGAACTGTTCCTGCAGTTCGGATATGTGTCACTGTTCTCTTGTG TCTACCCACTCGCTGCCGCTTTCGCCGTGTTGAACAACTTTACAGAAGTCAATAGCGACGCACTGAAGAT GTGCAGAGTCTTTAAACGGCCTTTCGCTGAACCCTCAGCATCAATCGGTGTCTGGCAGCTTGCGTTTGAG ACTATGTCCGTCATATCAGTGGTTACCAACTGCGCTCTGATCGGCATGTCTCCCCAGGTGAACGCAGTCT TCCCCGAATCAAAGACCGACCTTGTTCTGATCGTGGTAGCCGTGGAGCACGCTCTGCTTGCACTGAAATT TATACTTGCTTTTGCGATTCCTGATAAACCCCGGCATATACAGCAGAAACTGGCTCGGCTTGAGTTTGAG AGCTTGGAAGCCCTCAAGCAGCAACAAATGAAACTCGTCGCTGAAAACCTGAAGGAAGAATACCAGGAGG ACGGGAAGGAGGCCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219834 representing NM_133979
Red=Cloning site Green=Tags(s) MRVTLSTLDTCESSFTPLVVIELAQDVKDETKEWLKNRIIAKKKDGGAQLLFRPLLNKYEKETLENQNLY LVGASNVRLLLGAEAVGLVKECTDAAMRAFTYGTRHNFKGFHDNNNDFLTMAECQFIIKHELENLRARDE KMIPGYPQAKLYPGKSLMRRLLTSGIVTQVFPLHDTEALKKLEDTWYTRFALKYQPIDSIRSYFGETIAL YFGFLEYFTFALIPMAIIGLPYYLFVWEDYDKYVIFASFNLIWSTVILEVWKRGCANMTYRWGTLVMKRQ FEEPRPGFHGVLGINSVTGREEPLYSSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEDWALSLHEDS GSEWTSLLLYVPSIVYAVVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLVLKVLVFNFLNCFASLFYIA FVLKDMKLLRQSLATLLITSQILNQVVESLLPYWLQRKYCARVKRKVQALKSEVDTTLYEQVLLEKEMGT YLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFAEPSASIGVWQLAFE TMSVISVVTNCALIGMSPQVNAVFPESKTDLVLIVVAVEHALLALKFILAFAIPDKPRHIQQKLARLEFE SLEALKQQQMKLVAENLKEEYQEDGKEAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133979 |
| ORF Size | 1977 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133979.1, NM_133979.2, NM_133979.3, NP_598740.1 |
| RefSeq Size | 2653 bp |
| RefSeq ORF | 1980 bp |
| Locus ID | 102566 |
| UniProt ID | Q8BH79 |
| MW | 76.2 kDa |
| Gene Summary | This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to inhibit anion conductance. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220190 | Ano10 (untagged) - Mouse anoctamin 10 (Ano10), (10ug) |
CNY 8,460.00 |
|
| MG219834 | Ano10 (tGFP-tagged) - Mouse anoctamin 10 (Ano10), (10ug). Note: ORF is codon optimized |
CNY 7,000.00 |
|
| MR219834L3 | Lenti ORF clone of Ano10 (Myc-DDK-tagged) - Mouse anoctamin 10 (Ano10). Note: ORF is codon optimized |
CNY 8,460.00 |
|
| MR219834L4 | Lenti ORF clone of Ano10 (mGFP-tagged) - Mouse anoctamin 10 (Ano10). Note: ORF is codon optimized |
CNY 8,460.00 |
