Exd1 (NM_172857) Mouse Tagged ORF Clone
CAT#: MR219928
- TrueORF®
Exd1 (Myc-DDK-tagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1)
ORF Plasmid: tGFP
"NM_172857" in other vectors (4)
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CNY 4,248.00
CNY 4,560.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4932702D22Rik; Exdl1; mExd1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219928 representing NM_172857
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCAGCAGTGACTACCATTTCCTCAACCAGATTTTGTGGAGAAGGGTGAAGCTCACACTGGTGT CTGGCATCTTTGAGGGCGTGCTCCAGCACGTGGATCCTAACAAGATTGTTGTCCTGAAAAACGTGAGGAA TGCAGAGTCGGGCCGCAGCGTCCCAGGGGTGAAAGTGTTCTTCGGGCACGAGATCTTGAATGTGGAACTA ATGGATGAAGCAGAAGGTGCATCGGGGGAGAAGGCATCCGCTGTTAGCATAAATACAGAACGAGCTGGAA TGGAGAAAGTGAAAAATGAAGACGTCAATGTCTGTGAGCCGGCTTCACCTGCACCAGAGGTGCCAACCAG CTCTCTCCTCAGTGACCTCAAGTACTGCCCGTCAGAGGAAGAGGAGGTGACATACACAGTCATTGATCAG TTCCAGCAGAAGTTTGGTGCTGCAATGCTCCACATCAAGAAGCAGAGTGTCCTGAGTGTTGCTGCCGAAG GCGCCAATGTGTGTCGCCATGGGAAACTCTGTTGGCTTCAGGTGGCCACAAACAGCCGAGTTTACTTGTT TGATATTTTCCTTCTGGGAAGTCGTGCTTTCAATAATGGACTTCAGATGATCTTAGAAGACAAGAGAATC TTAAAGGTTATCCACGATTGTCGCTGGCTCTCTGACTGCCTGTCTCATCAGTATGGAATCATGCTGAACA ATGTCTTTGATACACAGGTAGCCGACGTCCTTCAATTTTCTATGGAAACGGGTGGCTTTCTTCCAAACTG TATCAGTACTTTGCAGGAGAGTTTAATCAGACACCTTAAAGTTGCTCCCAGATACCTTTTCTTTTTAGAA GAGAGACAGAAACGTATTCAGGAAAATCCAGAAATATGGTTGACAAGACCTCTTCCACCCTCTTTATTGA AAATCTTGGCCCTGGAAACAACCTACCTGCTTCCACTTCGATTGGTGCTCCTGGATGAGGTGATGTCAGA CTTAACCACACTGGTGGATGGGTACCTGAACACCTACCGGGAGGGCTCTGCAGACCGGCTTGCAGGCACA GAGCCTGCGTGTATGGAGCTGCCAGCAGAGCTTCTTCAACTCCAGGACTTCCAGAAACAGCGCAGAGAGC GAGCTGTGAAGGAGTACAGAGTGAACGCACGGGGCCTCCTCATAAGGACTCCACTGCACCCAAAGGAGCC GACAGCTTGCACAGCAGGGAAAGAGGAGCGAGTCCAGGGTTTCTTATTTTATAAGACGGATGGTGGAGAC CAAGTTCCACGCTTTTTATGTCCCAAATCTCATGAGGATGAGAAGTTTTTGGATAAAGAATCTAAACAAA CCACAGCAAAGTCTCAGATTGTGCCTCCCAGGAAGGAAGGCGAAGCCCATAAGGACTCTAAGAACAAACC GGGGTGTTGGGAGTCAGCGGGGCCAGAAGACCCGAGAGCCCAGAAAGCCCACGCCCTGCCTCCCACGTGG GCATCTCAGTCCCAGTTTTCTTTGAAAGAGGAGATAGAGCAGTTGACAGTGGTGGGAAATAAGGGAGCTT TAACAAGCCCAAAAGAAGGCGCTTTGGTGTCGCCTTCTCTCCTTCAGGAAACCTGGGAGGCACCAACTGA CACCTTCCATCTTCCTGAAAAAGCTGAGGTCTCTACACTTCCTCCCTGTCCAGCCCTGGAGAAGACAGAC TCATGGATAAGTCCATCTTTAAATCTGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219928 representing NM_172857
Red=Cloning site Green=Tags(s) MDPSSDYHFLNQILWRRVKLTLVSGIFEGVLQHVDPNKIVVLKNVRNAESGRSVPGVKVFFGHEILNVEL MDEAEGASGEKASAVSINTERAGMEKVKNEDVNVCEPASPAPEVPTSSLLSDLKYCPSEEEEVTYTVIDQ FQQKFGAAMLHIKKQSVLSVAAEGANVCRHGKLCWLQVATNSRVYLFDIFLLGSRAFNNGLQMILEDKRI LKVIHDCRWLSDCLSHQYGIMLNNVFDTQVADVLQFSMETGGFLPNCISTLQESLIRHLKVAPRYLFFLE ERQKRIQENPEIWLTRPLPPSLLKILALETTYLLPLRLVLLDEVMSDLTTLVDGYLNTYREGSADRLAGT EPACMELPAELLQLQDFQKQRRERAVKEYRVNARGLLIRTPLHPKEPTACTAGKEERVQGFLFYKTDGGD QVPRFLCPKSHEDEKFLDKESKQTTAKSQIVPPRKEGEAHKDSKNKPGCWESAGPEDPRAQKAHALPPTW ASQSQFSLKEEIEQLTVVGNKGALTSPKEGALVSPSLLQETWEAPTDTFHLPEKAEVSTLPPCPALEKTD SWISPSLNLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_172857 |
ORF Size | 1710 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172857.2, NP_766445.1 |
RefSeq Size | 3143 bp |
RefSeq ORF | 1713 bp |
Locus ID | 241624 |
UniProt ID | Q8CDF7 |
MW | 64 kDa |
Gene Summary | RNA-binding component of the PET complex, a multiprotein complex required for the processing of piRNAs during spermatogenesis. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposable elements, preventing their mobilization, which is essential for the germline integrity (PubMed:26669262). The PET complex is required during the secondary piRNAs metabolic process for the PIWIL2 slicing-triggered loading of PIWIL4 piRNAs. In the PET complex, EXD1 probably acts as an RNA adapter. EXD1 is an inactive exonuclease (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes.
,null,
Andrology
,PubMed ID 36624638
[Exd1]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219232 | Exd1 (untagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1), (10ug) |
CNY 7,320.00 |
|
MG219928 | Exd1 (tGFP-tagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1), (10ug) |
CNY 5,040.00 |
|
MR219928L3 | Lenti ORF clone of Exd1 (Myc-DDK-tagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1) |
CNY 6,460.00 |
|
MR219928L4 | Lenti ORF clone of Exd1 (mGFP-tagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1) |
CNY 6,460.00 |