Gpr174 (NM_001177782) Mouse Tagged ORF Clone
CAT#: MR220022
- TrueORF®
Gpr174 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 174 (Gpr174), transcript variant 3
ORF Plasmid: tGFP
"NM_001177782" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Gm376; Lypsr3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220022 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGATAATTTTACATGTAACAAGACAGATGGAGACAATACAGATTTCCGGTACTTCATTTATGCAG TGACATACACTGTTATTCTTGTGCCAGGTCTTATAGGGAACATATTAGCCTTATGGGTATTTTACGGCTA TATGAAAGAAACCAAAAGGGCTGTGGTATTTATGATAAACCTAGCCATTGCTGACTTATTACAGATTCTC TCTCTGCCACTGAGGATCTTTTACTACTTGAACCATGACTGGCCATTTGGTCCTGGTCTCTGCATGTTTT GTTTCTACCTGAAATATGTAAACATGTATGCCAGCATCTACTTCTTGGTCTGCATCAGTGTGCGAAGATT TTGGTTTCTCATGTACCCCTTTCGTTTCAATGACTGCAAACAGAAATATGACTTGTATATCAGCATTATT GGCTGGCTGATCATCTGCCTTGCCTGCCTGCTCTTTCCTCTCCTCAGAACCAATGATGATACTCCAGGTA ACAGAACCAAATGCTTTGTGGATCTTCCTATCAGGAATGTTAATCTGGCCCAATCTGTTGCCATGATAAC TATCGGAGAGGTGGTTGGGTTTGTTACTCCTCTTATGATTGTTTTATACTGTACCTGGAAGACAGCTTTG TCATTACAAAATAAATATCCCATTTCTCAACATCTTGGAGAGAAAAAGAAAGCCCTGAAGATGATTCTGA CCTGTGCAGGGGTGTTTCTAGTTTGCTTTGTACCTTATCACTTCAGCTTTCCTTTAGATTTCCTGGTTAA GTCCAATGAAATTAAGAGTTGCTTTGCCAGAAGAGTGATTCTAATATTTCATTCTGTGGCCCTGTGTCTG GCAAGCCTGAACTCCTGTCTTGACCCAGTTATATATTATTTTACCACTAATGAGTTCAGAAGACGCCTTT CAAGACAAGATTTGCCTGATAACATCCAACTCCATACAAAATCATACAAAATTGCAAGTAACCATGCCAC TTCTACCGTGGCAGCTGAACTATGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220022 protein sequence
Red=Cloning site Green=Tags(s) MTDNFTCNKTDGDNTDFRYFIYAVTYTVILVPGLIGNILALWVFYGYMKETKRAVVFMINLAIADLLQIL SLPLRIFYYLNHDWPFGPGLCMFCFYLKYVNMYASIYFLVCISVRRFWFLMYPFRFNDCKQKYDLYISII GWLIICLACLLFPLLRTNDDTPGNRTKCFVDLPIRNVNLAQSVAMITIGEVVGFVTPLMIVLYCTWKTAL SLQNKYPISQHLGEKKKALKMILTCAGVFLVCFVPYHFSFPLDFLVKSNEIKSCFARRVILIFHSVALCL ASLNSCLDPVIYYFTTNEFRRRLSRQDLPDNIQLHTKSYKIASNHATSTVAAELC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001177782 |
ORF Size | 1005 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001177782.1, NP_001171253.1 |
RefSeq Size | 4955 bp |
RefSeq ORF | 1008 bp |
Locus ID | 213439 |
UniProt ID | Q3U507 |
MW | 38.8 kDa |
Gene Summary | Putative receptor for purines coupled to G-proteins.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212593 | Gpr174 (untagged) - Mouse G protein-coupled receptor 174 (Gpr174), transcript variant 3, (10ug) |
CNY 3,990.00 |
|
MG220022 | Gpr174 (tGFP-tagged) - Mouse G protein-coupled receptor 174 (Gpr174) transcript variant 3, (10ug) |
CNY 3,140.00 |
|
MR220022L3 | Lenti ORF clone of Gpr174 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 174 (Gpr174), transcript variant 3 |
CNY 4,750.00 |
|
MR220022L4 | Lenti ORF clone of Gpr174 (mGFP-tagged) - Mouse G protein-coupled receptor 174 (Gpr174), transcript variant 3 |
CNY 4,750.00 |