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Mefv (NM_001161791) Mouse Tagged ORF Clone
CAT＃: MR220046
- TrueORF®
Mefv (Myc-DDK-tagged) - Mouse Mediterranean fever (Mefv), transcript variant 2
ORF Plasmid: tGFP
"NM_001161791" in other vectors (4)
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CNY 8,672.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FMF; pyrin; TRIM20 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220046 representing NM_001161791
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGACCCTTGGTGATCATCTGCTAAACACCCTGGAGGAGCTGCTGCCCTATGACTTTGAGAAGT TCAAGTTTAAGCTACAGAACACCAGCCTGGAGAAGGGCCACTCTAAGATCCCCCGGGGCCATATGCAGAT GGCCAGGCCAGTAAAGCTGGCCAGCCTTCTGATAACCTACTACGGGGAGGAGTATGCTGTAAGGCTGACC CTGCAGATCCTGCGGGCCACCAACCAGCGCCAACTAGCAGAGGAGCTTCGCAAAGCCACAGGCACAGAAC ATTTAATTGAAGAAAATAGAGTTGGTGGTTCTGTCCAGTCTTCTGTAGAGAATAAAGCCAAGAGTGTGAA GGTACCAGATGTCCCTGAAGGTGATGGGACACAGCAAAACAATGATGAATCAGACACCCTTCCATCCAGC CAGGCTGAAGTGGGGAAGGGACCCCAGAAGAAGTCACTGACCAAAAGGAAGGATCAGAGGGGCCCCGAGA GCCTGGACTCACAGACCAAGCCATGGACAAGGAGCACAGCGCCTCTCTATAGGAGAACCCAGGGCACCCA GTCCCCAGGGGATAAGGAGAGCACAGCAAGTGCCCAGCTCCGCAGGAATGTCAGCTCTGCAGGGAGGCTT CAAGGACTTTACAACAATGCCCCAGGGAGGAGAGAAAGCAAGAAAGCCGAAGTGTATGTATATTTGCCTT CAGGAAAGAAGCGACCCAGGAGTCTTGAAATTACCACTTATTCAAGAGAAGGAGAACCCCCAAATTCAGA AGTTCTTCCAACTCAAGAGGAAACAAGAAATGGGAGTCTCATTCGCATGAGAACAGCTACTCTGAATGGA AGGACTACGGGGGCTTTAGAAAAGGGCACAGGGATTCCAGAGCATTCCATGGTGCTGGATGAAAAAACAT TCAGAAACATGTCTTCCAAAACATCGTTGATTGGGGAGGAGAGATGCCCTACATCATGGACGGAAAATGG AAATGGGAGTCCAGAAACCACAGAGTCCTCGGGAGAGACGGCTGGCAGCATACTCTCTGATCCAGAAGTG CCTTTGTCACTATGTGAAAAACCAGCTAAAACTCCAGAAGACCCAGCATCCTTAGGACAGGCCGCTTGTG AAGGAAGGTCACAGGACAAGGCTGTGTGCCCTCTTTGCCACACCCAGGAAGGAGACCTGCGTGGTGATAC CTGTGTGCAAAGTTCCTGTAGCTGCTCCATTGCTCCTGGGGATCCCAAGGCCTCCGGCAGATGCTCCATA TGCTTTCAGTGCCAAGGCTTACTTGCCAGAAAGAGCTGCGAAGCCCAGAGCCCCCAGTCCCTACCACAGT GCCCTCGTCACATGAAGCAGGTGCTGCTGCTTTTCTGTGAGGACCACAGGGAGCCCATCTGCCTCATCTG CAGGCTGAGCCTGGAGCATCAAGGACATCGAGTGCGCCCCATAGAGGAGGCTGCACTAGAGTACAAGGAG CAGATCCGGGAGCAGCTGGAGCGCCTGCGGGAGATGAGGGGATATGTGGAGGAGCACAGGCTACAGGGAG ACAAGAAAACAGACGATTTCCTGAAACAAACAGAAATCCAGAAGCAGAAGATTTCATGTCCACTTGAGAA GCTATACCAACTCCTGGAGAAGCAAGAACAACTCTTTGTGACCTGGCTACAGGAGCTAAGCCAGACCATC AGCAAGGTCAGAGAGACATACTACACCCGGGTTTCCCTACTGGATGAGATGATTGAAGAACTAGAGGCCA AGCAAGACCAGCCAGAGTGGGACCTCATGCAGGACATTGGAATCACCTTGCACAGGGCTAAGATGATGTC TGCCTCTGAGCTGTTGGACACTCCTCCAGGTGTTAAAGAAAAGCTTCATCTGCTCTACCAGAAGTCAAAG TCTGTGGAGAAGAATATGCAGTGTTTCTCAGAAATGCTGAGCTCTGAAATGGCATTCAGCGCTTCAGATG TAGCAAAATGGGAAGGGCGCCAGCCTAGCGCCACACAGGTCCAGGGCTTGGTTCCCACCGTTCACCTAAA GTGTGATGGTGCACACACACAGGACTGTGATGTAGTATTTTACCCAGAACGGGAAGCTGGAGGATCAGAA CCTAAAGATTACCTTCATCCTCAGCCAGCTCAAGACACACCTGAGCTACATGAGATCCATTCTCGGAACA ACAAAAGAAAATTTAAATCTTTCCTGAAATGGAAACCTTCATTCTTACCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220046 representing NM_001161791
Red=Cloning site Green=Tags(s) MAKTLGDHLLNTLEELLPYDFEKFKFKLQNTSLEKGHSKIPRGHMQMARPVKLASLLITYYGEEYAVRLT LQILRATNQRQLAEELRKATGTEHLIEENRVGGSVQSSVENKAKSVKVPDVPEGDGTQQNNDESDTLPSS QAEVGKGPQKKSLTKRKDQRGPESLDSQTKPWTRSTAPLYRRTQGTQSPGDKESTASAQLRRNVSSAGRL QGLYNNAPGRRESKKAEVYVYLPSGKKRPRSLEITTYSREGEPPNSEVLPTQEETRNGSLIRMRTATLNG RTTGALEKGTGIPEHSMVLDEKTFRNMSSKTSLIGEERCPTSWTENGNGSPETTESSGETAGSILSDPEV PLSLCEKPAKTPEDPASLGQAACEGRSQDKAVCPLCHTQEGDLRGDTCVQSSCSCSIAPGDPKASGRCSI CFQCQGLLARKSCEAQSPQSLPQCPRHMKQVLLLFCEDHREPICLICRLSLEHQGHRVRPIEEAALEYKE QIREQLERLREMRGYVEEHRLQGDKKTDDFLKQTEIQKQKISCPLEKLYQLLEKQEQLFVTWLQELSQTI SKVRETYYTRVSLLDEMIEELEAKQDQPEWDLMQDIGITLHRAKMMSASELLDTPPGVKEKLHLLYQKSK SVEKNMQCFSEMLSSEMAFSASDVAKWEGRQPSATQVQGLVPTVHLKCDGAHTQDCDVVFYPEREAGGSE PKDYLHPQPAQDTPELHEIHSRNNKRKFKSFLKWKPSFLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001161791 |
| ORF Size | 2220 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001161791.1, NP_001155263.1 |
| RefSeq Size | 3094 bp |
| RefSeq ORF | 2223 bp |
| Locus ID | 54483 |
| MW | 83.6 kDa |
| Gene Summary | Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it may also have a positive effect in the inflammatory pathway. In different experimental systems, it has been shown to activate IL1B production. It has also been shown to be required for PSTPIP1-induced PYCARD oligomerization and for formation of inflammasomes. Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221233 | Mefv (untagged) - Mouse Mediterranean fever (Mefv), transcript variant 2, (10ug) |
CNY 9,600.00 |
|
| MG220046 | Mefv (tGFP-tagged) - Mouse Mediterranean fever (Mefv) transcript variant 2, (10ug) |
CNY 8,170.00 |
|
| MR220046L3 | Lenti ORF clone of Mefv (Myc-DDK-tagged) - Mouse Mediterranean fever (Mefv), transcript variant 2 |
CNY 9,310.00 |
|
| MR220046L4 | Lenti ORF clone of Mefv (mGFP-tagged) - Mouse Mediterranean fever (Mefv), transcript variant 2 |
CNY 9,310.00 |
