United States
Germany
Japan
United Kingdom
China
Chkb (NM_007692) Mouse Tagged ORF Clone
CAT＃: MR220968
- TrueORF®
Chkb (Myc-DDK-tagged) - Mouse choline kinase beta (Chkb), transcript variant 1
ORF Plasmid: tGFP
"NM_007692" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,900.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Chetk; Chkl; Ck; Ck/Ek; Ck/Ek-beta; Ckb; Ckekb; Ek |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220968 representing NM_007692
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGACGGGACAGGGGTAGTCGGAGGAGGGGCTGTCGGCGGCGGCCTGCCCAAGGACGGTTTGC AGGATGCTAAGTGCCCAGAGCCAATCCCCAATCGGCGGCGCGCTTCCTCGCTGTCCCGTGACGCGCAGCG CCGAGCCTATCAGTGGTGCCGGGAGTACCTGGGCGGGGCCTGGCGCCGAGCGCGGCCGGAGGAGCTGAGC GTTTGTCCCGTGAGCGGAGGCCTCAGCAACCTGCTCTTCCGATGCTCACTACCGAACCACGTACCCAGTG TGGGCGGGGAGCCCCGGGAGGTGCTGCTACGACTCTACGGGGCCATCCTGCAGGGTGTAGACTCCTTGGT ATTAGAAAGCGTGATGTTCGCCATTCTCGCAGAGCGGTCTCTAGGGCCCCAGCTTTACGGAGTGTTTCCA GAGGGCCGCTTGGAACAGTACCTCCCAAGCCGGCCGTTGAAAACTCAAGAGCTCCGGGACCCAGTGTTGT CGGGAGCCATTGCAACGAGGATGGCCCGTTTCCATGGTATGGAGATGCCCTTCACTAAGGAGCCCCGATG GTTGTTTGGGACCATGGAGCGGTACCTGAAGCAGATCCAGGACTTGCCTTCCACCAGCCTTCCCCAGATG AACCTGGTAGAGATGTACAGCCTCAAGGACGAGATGAACAGCCTCAGGAAGTTACTAGACGATACCCCGT CACCAGTGGTCTTCTGCCACAATGACATCCAGGAAGGAAACATCTTGTTGCTTTCAGAGCCAGACAGTGA TGATAACCTCATGTTGGTTGATTTTGAGTACAGTAGTTATAACTACAGGGGCTTTGACATTGGGAATCAT TTTTGTGAGTGGGTTTATGATTATACTTATGAAGAATGGCCTTTCTACAAAGCAAGACCCACGGACTACC CCACTAGAGAACAGCAGCTCCATTTTATTCGCCATTATCTGGCAGAGGTTCAGAAGGGTGAGATCCTCTC CGAAGAGGAACAGAAGAAACGGGAAGAAGAATTGCTGCTAGAGATCAGTCGGTACTCCCTGGCATCTCAC TTTTTCTGGGGTCTGTGGTCCACCCTGCAGGCTTCGATGTCCACTATAGAGTTCGGCTACTTGGAGTATG CCCAATCTCGGTTCCAGTTCTACTTCCAGCAGAAGGGCCAGCTGACGAGTTCCCCATCATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220968 representing NM_007692
Red=Cloning site Green=Tags(s) MAADGTGVVGGGAVGGGLPKDGLQDAKCPEPIPNRRRASSLSRDAQRRAYQWCREYLGGAWRRARPEELS VCPVSGGLSNLLFRCSLPNHVPSVGGEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFP EGRLEQYLPSRPLKTQELRDPVLSGAIATRMARFHGMEMPFTKEPRWLFGTMERYLKQIQDLPSTSLPQM NLVEMYSLKDEMNSLRKLLDDTPSPVVFCHNDIQEGNILLLSEPDSDDNLMLVDFEYSSYNYRGFDIGNH FCEWVYDYTYEEWPFYKARPTDYPTREQQLHFIRHYLAEVQKGEILSEEEQKKREEELLLEISRYSLASH FFWGLWSTLQASMSTIEFGYLEYAQSRFQFYFQQKGQLTSSPSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007692 |
| ORF Size | 1182 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007692.6, NP_031718.1 |
| RefSeq Size | 1707 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 12651 |
| UniProt ID | O55229 |
| MW | 45.6 kDa |
| Gene Summary | Has a key role in phospholipid metabolism, and catalyzes the first step of phosphatidylethanolamine and phosphatidylcholine biosynthesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208282 | Chkb (untagged) - Mouse choline kinase beta (Chkb), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG220968 | Chkb (tGFP-tagged) - Mouse choline kinase beta (Chkb), (10ug) |
CNY 5,624.00 |
|
| MR220968L3 | Lenti ORF clone of Chkb (Myc-DDK-tagged) - Mouse choline kinase beta (Chkb), transcript variant 1 |
CNY 5,800.00 |
|
| MR220968L4 | Lenti ORF clone of Chkb (mGFP-tagged) - Mouse choline kinase beta (Chkb), transcript variant 1 |
CNY 5,800.00 |
