Rnf166 (NM_001033142) Mouse Tagged ORF Clone
CAT#: MR221146
- TrueORF®
Rnf166 (Myc-DDK-tagged) - Mouse ring finger protein 166 (Rnf166)
ORF Plasmid: tGFP
"NM_001033142" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1110031E24Rik; AW555115; Zfp313l |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR221146 representing NM_001033142
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGTTCCGCAGCCTGGTGGCCTCGGCGCAGCAGCGGCAGCCGCCGGCTGGGCCCGCGGGCGGCG ACAGCGGCCTGGAGGCGCAGTTCAGCTGTCCCATCTGCCTGGAGGTGTACCATCGGCCAGTGGCCATCGG CAGCTGCGGCCACACGTTCTGCGGGGAGTGCCTCCAGCCATGTCTGCAGGTGCCGTCCCCTCTGTGCCCA CTCTGCCGTCTGCCCTTCGACCCCAAGAAAGTGGACAAGGCCACCCATGTAGAGAAGCAACTCTCATCCT ACAAGGCACCCTGTCGGGGTTGCAACAAGAAGGTGACACTGGCCAAGATGAGAGCGCACATTTCCTCCTG CCTGAAGGTCCAGGAGCAGATGGCTAACTGCCCCAAATTCGTCCCTGTGGTGCCCACATCTCAGCCCATC CCCAGCAATATCCCCAACCGGTCTACCTTCGCCTGCCCCTACTGCGGAGCCCGCAATCTGGATCAGCAGG AGCTGGTGAAGCACTGCGTGGAAAGTCACCGCAGTGATCCCAACCGTGTGGTATGCCCCATCTGCTCAGC AATGCCCTGGGGGGACCCCAGCTACAAAAGCGCCAACTTCCTGCAGCATCTGCTGCACCGGCACAAGTTC TCCTACGACACCTTCGTGGACTACAGCATCGACGAAGAAGCTGCCTTCCAGGCTGCCCTGGCTCTGTCCC TCTCTGAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221146 representing NM_001033142
Red=Cloning site Green=Tags(s) MAMFRSLVASAQQRQPPAGPAGGDSGLEAQFSCPICLEVYHRPVAIGSCGHTFCGECLQPCLQVPSPLCP LCRLPFDPKKVDKATHVEKQLSSYKAPCRGCNKKVTLAKMRAHISSCLKVQEQMANCPKFVPVVPTSQPI PSNIPNRSTFACPYCGARNLDQQELVKHCVESHRSDPNRVVCPICSAMPWGDPSYKSANFLQHLLHRHKF SYDTFVDYSIDEEAAFQAALALSLSEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001033142 |
ORF Size | 711 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033142.3 |
RefSeq Size | 1783 bp |
RefSeq ORF | 714 bp |
Locus ID | 68718 |
UniProt ID | Q3U9F6 |
MW | 26.1 kDa |
Gene Summary | E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates. In turn, participates in different biological processes including interferon production or autophagy. Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6. Plays also a role in the early recruitment of autophagy adapters to bacteria. Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210783 | Rnf166 (untagged) - Mouse ring finger protein 166 (Rnf166), (10ug) |
CNY 3,990.00 |
|
MG221146 | Rnf166 (tGFP-tagged) - Mouse ring finger protein 166 (Rnf166), (10ug) |
CNY 2,850.00 |
|
MR221146L3 | Lenti ORF clone of Rnf166 (Myc-DDK-tagged) - Mouse ring finger protein 166 (Rnf166) |
CNY 4,750.00 |
|
MR221146L4 | Lenti ORF clone of Rnf166 (mGFP-tagged) - Mouse ring finger protein 166 (Rnf166) |
CNY 4,750.00 |