Myh13 (NM_001081250) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MyHC-eo
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221295 representing NM_001081250 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCAGACGCAGAGATGGCCATATTTGGAGAAGCCGCTCCCTACCTCCGAAAACCAGAGAAGGAGA GGATCGAGGCTCAGAACCGCCCATTCGACTCGAAGAAAGCCTGCTTTGCTGTGGATGATAAAGAAATGTA CGTGAAAGGCATGATCCAAAGCAGGGAAAATGACAAAGTCATTGTCAAGACCCTAGACGACCGGGAGCTC ACTCTGAACAGTGACCAAGTCTTCCCCATGAACCCCCCTAAGTTTGACAAGATCGAGGACATGGCCATGA TGACCCACCTGCACGAGCCCGCTGTGCTGTACAATCTCAAAGAGCGTTATGCAGCCTGGATGATCTATAC CTACTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAGGTGGTG GCGGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAATGCCTACC AGTTCATGCTAACAGATCGAGATAACCAATCCATCTTGATCACTGGAGAATCTGGGGCTGGAAAGACAGT GAACACGAAGCGTGTCATCCAATACTTTGCAACAATTGCAGTCACCGGGGACAAGAAGAAAGAGCAGCAG CCGGGCAAAATGCAGGGAACGCTGGAGGATCAGATCATCCAAGCCAACCCCCTGCTGGAGGCCTTTGGAA ACGCCAAGACCGTGAGGAACGACAACTCCTCCAGATTTGGGAAGTTCATTCGGATCCACTTTGGAGCCAC AGGGAAGCTAGCATCAGCGGACATCGAAACCTATCTCCTGGAAAAATCCAGAGTGACATTCCAGTTATCC AGTGAAAGAAGCTATCACATTTTTTATCAAATCATGTCAAATAAGAAGCCAGAACTAATTGACTTGCTTC TGATTTCAACCAACCCCTTCGACTTCCCTTTCGTGAGCCAAGGAGAGGTCACAGTAGCTAGCATCGATGA CAGCGAGGAATTGTTGGCCACTGATAATGCCATTGACATCCTGGGCTTTAGCCCGGAGGAGAAAGTTGGG ATCTACAAGCTGACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAACAGAAGCAGAGAGAGGAGC AGGCTGAGCCAGATGGCACGGAAGTGGCTGACAAAGCTGGATACCTGATGGGACTGAATTCTGCAGAGAT GCTGAAAGGCCTGTGCTGTCCAAGAGTGAAGGTTGGAAATGAGTATGTCACCAAAGGACAGAATGTCCAG CAGGTGACCAACTCAGTGGGTGCCCTGGCCAAGGCTGTCTATGAGAAGATGTTCCTATGGATGGTCACCC GCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATCGCTGGCTT TGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAGTTT TTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCATTG ACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATTGAGAAGCCAATGGGCATCTTCTCCATCCTGGA AGAGGAGTGCATGTTCCCTAAGGCCACAGACACCTCCTTCAAGAACAAGTTGTATGACCAGCACCTGGGA AAGTCCAACAACTTCCAGAAGCCTAAACCCACCAAAGGCAAGGCCGAAGCCCACTTCTCCCTGGTGCACT ATGCGGGCACTGTGGATTACAACATCGCTGGCTGGCTGGATAAGAACAAGGACCCACTGAATGAGACCGT GGTGGGGCTGTACCAGAAGTCCTCCCTGAAGTTGTTGTCCTTCCTCTTTTCCAACTACGCAGGAGCAGAA GCAGGTGACTCCGCAGGAGGCAAGAAGGGAGGAAAGAAGAAGGGCTCCTCCTTCCAGACTGTGTCTGCTG TGTTCAGGGAAAACTTAAACAAATTGATGACCAACCTAAGGAGCACCCATCCTCACTTTGTGCGATGCCT GATTCCCAATGAGACCAAGACTCCTGGTGTGATGGACCACTACTTGGTCATGCATCAGCTGCGCTGTAAT GGGGTCCTGGAGGGCATCCGGATCTGCAGGAAAGGGTTCCCCAGTCGAATCCTCTATGCTGACTTCAAAC AACGGTACCGGATCCTTAACGCCAGTGCCATCCCGGAAGGGCAGTTCATCGACAGCAAGAACGCCTCGGA GAAGCTCCTGAACTCCATCGATGTGGACCGAGAGCAGTTCCGATTCGGCCATACCAAGGTGTTTTTCAAA GCTGGGCTCCTGGGGCTTCTGGAAGAGATGAGAGACGAGAAGCTGGTGACCCTAATGACACGCACACAGG CAGTGTGCAGGGGCTACCTGATGAGGGTGGAGTTCAAGAAAATGATGGAGAGGAGGGAGTCCATCTTCTG CATCCAGTACAATGTTCGTTCCTTCATGAACGTCAAGCACTGGCCCTGGATGAACCTGTTCTTCAAGATC AAGCCCCTGCTGAAGAGCGCGGAGGCGGAGAAGGAGATGGCCACCATGAAGGAGGACTTCGAACGAGCCA AGGAAGACCTGGCTAGATCTGAGGCTCGTCGGAAGGAGCTGGAGGAGAAAATGGTCTCCCTGCTGCAGGA GAAAAATGACCTTCAGTTGCAAGTCCAGTCTGAAACAGAGAATCTAATGGATGCGGAGGAACGATGCGAG GGTCTCATCAAAAGCAAGATCCAACTGGAGGCAAAGGTCAAGGAACTGAATGAGAGGCTGGAGGAGGAGG AAGAGATGAATTCTGAGCTGGTTGCCAAGAAGAGGAATCTGGAAGACAAGTGTTCTTCTCTCAAAAGAGA CATTGACGACCTGGAGTTGACCCTGACTAAGGTCGAGAAGGAAAAGCACGCCACGGAGAACAAGGTGAAG AACCTCTCTGAAGAAATGACAGCGCTTGAGGAAACCATTTCCAAACTGACGAAAGAAAAGAAGTCTCTGC AGGAAGCCCATCAGCAGACCCTGGATGACCTGCAGGTTGAAGAAGATAAAGTCAACGGTCTCATCAAAAT CAATGTCAAGCTGGAACAGCAGACGGATGACCTCGAGGGCAGCTTGGAGCAGGAGAAGAAACTGCGGGCT GACCTGGAGAGAGTGAAGAGGAAGCTGGAAGGAGACTTGAAGATGTCCCAGGAATCCATCATGGATCTAG AGAATGACACGCAACAGTTGGAGGAGAAACTGAAGAAGAAGGAGTTTGAAATGAGTCAGTTACAAACCAG AATCGATGATGAGCAAGTCCTCAGTCTACAGCTGCAGAAGAAGATCAAAGAATTACAGGCCCGCACAGAA GAGCTAGAGGAAGAAATTGAAGCAGAGCACACAGTCAGAGCCAAAATTGAGAAGCAGCGCTCAGACCTAG CCAGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCAGTGGGGCTACTTCAGCCCAGATCGAGAT GAACAAGAAGAGAGAATCCGAGTTCCAGAAACTTCGAAGGGACCTGGAGGAGGCCACACTGCAGCACGAA GCCACAGCAGCCACCCTGAGGAAGAAGCACGCGGACACCGTGGCGGAGCTCGGGGAGCAGATCGACAACC TGCAGAGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGATGACATGGCCAG CAACATTGAAACAGTCTCCAAGTCAAAGAGTAACATGGAAAGAATGTGCCGGTCTGTGGAAGACCAATTT AACGAAATCAAAGCCAAGGATGACCAACAGACCCAGTTAATCCACGATCTGAACATGCAGAAGGCAAGGC TGCAGACCCAGAATGGCGAGCTGAGCCACCAAGTCGAAGAGAAGGAGTCTCTGGTTTCACAGCTCACCAA AAGCAAGCAGGCCCTCACCCAGCAGCTGGAGGAGCTTAAGAGGCAACTGGAGGAAGAAACCAAGGCCAAG AACGCGCTGGCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGG AGCAGGAAGGCAAAGCCGAGCTGCAGAGGGCACTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGAC AAAATATGAGACGGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAAAAGCTGGCTCAGAGG CTCCAGGAGGCAGAGGAAAACACCGAGGCATCCAACTCCAAGTGTGCCTCCCTGGAGAAAACCAAGCAGA GGCTTCAGGGAGAAGTGGATGACCTGATGCTGGACTTGGAGAGGGCCAACACAGCCTGCGCCACCCTGGA CAAGAAACAAAGAAACTTCGACAAGGTCCTTGCAGAGTGGAAACAAAAACTGGATGAGAGCCAAGCTGAG CTGGAAGCTGCCCAGAAAGAGTCCAGGTCTCTCAGTACTGAAATCTTCAAGATGAGGAATGCCTATGAGG AGGTGGTGGATCAGTTAGAGACACTGAGACGGGAGAATAAAAACCTGCAAGAGGAGATTTCTGACTTAAC AGAGCAGATTGCAGAAACGGGCAAAAACCTCCAGGAAGTGGAAAAGACAAAGAAGCAAGTGGAGCAGGAG AAATCTGACCTCCAGGCTGCCTTGGAAGAGGTGGAGGGTTCCCTGGAACACGAGGAGAGCAAGATCTTGC GAGTCCAGCTAGAGTTAAGCCAGGTAAAATCTGAGCTTGATCGCAAGGTCACAGAAAAGGATGAGGAAAT CGAACAAATAAAAAGAAACAGCCAGCGTGCTGTGGAGGCCATGCAAAGTGTGTTAGATGCAGAAATCCGA AGCCGCAATGACGCCCTGAGACTGAAGAAGAAGATGGAGGGTGATCTCAACGAGATGGAGATTCAGCTGA GCCATGCCAACCGCCAGGTGGCAGAGACCCAGAAACACTTGCGCACGGTCCAAGGCCAGCTTAAGGATTC CCAGCTGCACCTGGACGATGCCCAGAGAAGTAACGAGGACCTCAAGGAGCAGCTAGCCATTGTGGAGCGC AGGAATGGCCTCCTGCAGGAGGAGCTGGAGGAAATGAAGGTGGCCCTGGAGCAGACAGAGCGGACCCGCA GGCTGTCAGAGCAGGAGCTGCTGGACTCCAGTGACCGTGTCCAGCTGCTCCATTCCCAGAACACGAGCCT GATAAATACCAAGAAAAAACTAGAGGCCGACTTGGCTCAATGCCAGGCAGAAGTGGAGAACTCCATCCAG GAGTCCAGAAACGCAGAAGAGAAGGCCAAGAAGGCCATCACTGACGCCGCCATGATGGCGGAGGAGCTGA AGAAGGAGCAGGACACCAGCGCCCACCTGGAGAGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCT GCAGCACCGTCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAG GCCAGGGTGCGGGAGTTGGAAAGCGAGCTGGATGCTGAGCAGAAGAGAGGAGCTGAAGCTCTGAAGGGAG CCCACAAATATGAGCGCAAAGTTAAAGAGATGACTTACCAGGCCGAGGAGGACCGCAAGAACATCCTCCG GCTCCAGGACCTGGTGGACAAGCTGCAGGCCAAAGTGAAGTCCTACAAGAGGCAGGCAGAGGAGGCTGAG GAGCAAGCCAACACACAGCTATCCAGGTGCCGGAGGGTCCAGCACGAGCTAGAGGAGGCTGAGGAGAGGG CAGACATCGCTGAGTCTCAAGTCAACAAGCTAAGGGCCAAGAGCCGTGATGTGGGAGGCCAGAAGATGGA AGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221295 representing NM_001081250 Red=Cloning site Green=Tags(s) MSSDAEMAIFGEAAPYLRKPEKERIEAQNRPFDSKKACFAVDDKEMYVKGMIQSRENDKVIVKTLDDREL TLNSDQVFPMNPPKFDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVV AAYRGKKRQEAPPHIFSISDNAYQFMLTDRDNQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKEQQ PGKMQGTLEDQIIQANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLS SERSYHIFYQIMSNKKPELIDLLLISTNPFDFPFVSQGEVTVASIDDSEELLATDNAIDILGFSPEEKVG IYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAGYLMGLNSAEMLKGLCCPRVKVGNEYVTKGQNVQ QVTNSVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQF FNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLG KSNNFQKPKPTKGKAEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSSLKLLSFLFSNYAGAE AGDSAGGKKGGKKKGSSFQTVSAVFRENLNKLMTNLRSTHPHFVRCLIPNETKTPGVMDHYLVMHQLRCN GVLEGIRICRKGFPSRILYADFKQRYRILNASAIPEGQFIDSKNASEKLLNSIDVDREQFRFGHTKVFFK AGLLGLLEEMRDEKLVTLMTRTQAVCRGYLMRVEFKKMMERRESIFCIQYNVRSFMNVKHWPWMNLFFKI KPLLKSAEAEKEMATMKEDFERAKEDLARSEARRKELEEKMVSLLQEKNDLQLQVQSETENLMDAEERCE GLIKSKIQLEAKVKELNERLEEEEEMNSELVAKKRNLEDKCSSLKRDIDDLELTLTKVEKEKHATENKVK NLSEEMTALEETISKLTKEKKSLQEAHQQTLDDLQVEEDKVNGLIKINVKLEQQTDDLEGSLEQEKKLRA DLERVKRKLEGDLKMSQESIMDLENDTQQLEEKLKKKEFEMSQLQTRIDDEQVLSLQLQKKIKELQARTE ELEEEIEAEHTVRAKIEKQRSDLARELEEISERLEEASGATSAQIEMNKKRESEFQKLRRDLEEATLQHE ATAATLRKKHADTVAELGEQIDNLQRVKQKLEKEKSELKMEIDDMASNIETVSKSKSNMERMCRSVEDQF NEIKAKDDQQTQLIHDLNMQKARLQTQNGELSHQVEEKESLVSQLTKSKQALTQQLEELKRQLEEETKAK NALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQR LQEAEENTEASNSKCASLEKTKQRLQGEVDDLMLDLERANTACATLDKKQRNFDKVLAEWKQKLDESQAE LEAAQKESRSLSTEIFKMRNAYEEVVDQLETLRRENKNLQEEISDLTEQIAETGKNLQEVEKTKKQVEQE KSDLQAALEEVEGSLEHEESKILRVQLELSQVKSELDRKVTEKDEEIEQIKRNSQRAVEAMQSVLDAEIR SRNDALRLKKKMEGDLNEMEIQLSHANRQVAETQKHLRTVQGQLKDSQLHLDDAQRSNEDLKEQLAIVER RNGLLQEELEEMKVALEQTERTRRLSEQELLDSSDRVQLLHSQNTSLINTKKKLEADLAQCQAEVENSIQ ESRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLE ARVRELESELDAEQKRGAEALKGAHKYERKVKEMTYQAEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAE EQANTQLSRCRRVQHELEEAEERADIAESQVNKLRAKSRDVGGQKMEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001081250
ORF Size	5814 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081250.2
RefSeq Size	5817 bp
RefSeq ORF	5817 bp
Locus ID	544791
MW	224 kDa

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