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Prrt1 (NM_030890) Mouse Tagged ORF Clone
CAT＃: MR221410
- TrueORF®
Prrt1 (Myc-DDK-tagged) - Mouse proline-rich transmembrane protein 1 (Prrt1)
ORF Plasmid: tGFP
"NM_030890" in other vectors (4)
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CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Bat-4; Bat4; D17H6S54E; DSPD1; G5b; NG5; ORF31 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221410 representing NM_030890
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCAGAAAAGTCAGGCCTTCCAGACTCGGTTCCCCACACTTCACCGCCGCCCTACAATGCCCCCC AGCCACCGGCCGAGCCCCCCATCCCGCCCCCACAAACGGCCCCATCCTCACATCACCACCACCACCACCA TTACCACCAGTCTGGCACCGCCACCCTCCCGCGCTTAGGAGCAGGTGGCCTGGCCTCTGCTGCGGCCAGT GCTCAACGCGGTCCTTCGTCTTCCGCCACGCTACCGCGGCCCCCCCACCATGCCCCGCCCGGCCCCGCCG CTGGGGCTCCCCCACCCGGCTGTGCTACCTTACCCCGCATGCCACCCGACCCTTATCTGCAGGAGACTCG CTTCGAGGGTCCACTTCCCCCACCACCGCCTGCGGCCGCCGCCCCGCCCCCACCAGCGCCTGCCCCGACG GCCCAAGCCCCGGGCTTCGTGGTTCCCACGCACGCGGGGGCGGTGGGCACGTTGCCGCTGGGGGGCTACG TGGCGCCGGGCTACCCGCTGCAGCTGCAGCCGTGCACTGCTTATGTCCCTGTGTATCCGGTGGGCACGCC CTACGCAGGCGGGACCCCCGGGGGTCCTGGAGTGACCTCCACTCTGCCCCCGCCGCCCCAGGGCCCAGGA TTGGCTCTGTTGGAGCCCAGGCGTCCGCCACACGACTACATGCCCATCGCTGTGCTGACCACCATCTGCT GCTTCTGGCCCACAGGCATCATCGCCATCTTCAAGGCCGTGCAGGTGCGCACGGCTTTGGCCCGCGGAGA TTTGGTGTCTGCCGAGATCGCTTCACGCGAAGCCCGGAATTTCTCCTTCATCTCCCTGGCCGTGGGCATC GCAGCCATGGTGCTTTGTACCATCCTCACTGTAGTCATCATCATCGCCGCCCAGCACCACGAAAACTACT GGGATCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221410 representing NM_030890
Red=Cloning site Green=Tags(s) MSSEKSGLPDSVPHTSPPPYNAPQPPAEPPIPPPQTAPSSHHHHHHHYHQSGTATLPRLGAGGLASAAAS AQRGPSSSATLPRPPHHAPPGPAAGAPPPGCATLPRMPPDPYLQETRFEGPLPPPPPAAAAPPPPAPAPT AQAPGFVVPTHAGAVGTLPLGGYVAPGYPLQLQPCTAYVPVYPVGTPYAGGTPGGPGVTSTLPPPPQGPG LALLEPRRPPHDYMPIAVLTTICCFWPTGIIAIFKAVQVRTALARGDLVSAEIASREARNFSFISLAVGI AAMVLCTILTVVIIIAAQHHENYWDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030890 |
| ORF Size | 918 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030890.2 |
| RefSeq Size | 921 bp |
| RefSeq ORF | 921 bp |
| Locus ID | 260297 |
| UniProt ID | O35449 |
| MW | 31.8 kDa |
| Gene Summary | Required to maintain a pool of extrasynaptic AMPA-regulated glutamate receptors (AMPAR) which is necessary for synapse development and function (PubMed:29490264). Regulates AMPAR function and synaptic transmission during development but is dispensable at mature hippocampal synapses (PubMed:29490264, PubMed:31216424). Plays a role in regulating basal phosphorylation levels of glutamate receptor GRIA1 and promotes GRIA1 and GRIA2 cell surface expression (PubMed:31216424).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC214247 | Prrt1 (untagged) - Mouse proline-rich transmembrane protein 1 (Prrt1), (10ug) |
CNY 2,400.00 |
|
| MG221410 | Prrt1 (tGFP-tagged) - Mouse proline-rich transmembrane protein 1 (Prrt1), (10ug) |
CNY 2,850.00 |
|
| MR221410L3 | Lenti ORF clone of Prrt1 (Myc-DDK-tagged) - Mouse proline-rich transmembrane protein 1 (Prrt1) |
CNY 4,750.00 |
|
| MR221410L4 | Lenti ORF clone of Prrt1 (mGFP-tagged) - Mouse proline-rich transmembrane protein 1 (Prrt1) |
CNY 4,750.00 |
