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Secisbp2 (NM_029279) Mouse Tagged ORF Clone
CAT＃: MR221507
- TrueORF®
Secisbp2 (Myc-DDK-tagged) - Mouse SECIS binding protein 2 (Secisbp2)
ORF Plasmid: tGFP
"NM_029279" in other vectors (4)
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CNY 6,288.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2210413N07Rik; 2810012K13Rik; SB; SBP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221507 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGAGCGGCCGCGGGAGCCGGACGGCGAGGATAGCATCAAGTTGTCAGCTGATGTCAAACCAT TCGTCCCTAAGTTTGCTGGGCTCAACGTGGCGTGGTCAGAGTCCTCAGAGACACGTGTCTTCCCAGGCTG TGCGGCCACCTACTATCCATTTGTACAGGAACCACCAGCAGCTGAACAGAAAATGTATCCCGAAGACATG GCTTTCGGAGCCCCCACCTTTCCAGCACAGTACGTGTCTTCTGAGATAGCGCTGCATCCTTTTGCCTATC CCACTTACACCCTAGAGTCCGCACAGAGTGTTTGCTCAGTGCCAACCCTGCAGTACGACTACAGCCAAGC ACGGTGTCACCCAGGCTTTCGGACAGCAAAGCCCCGGCATGAGCACGTGTGCCCTCCACCTCAGGAAGCA AAAGGTGTATTTAAGAAAAAACCCTCTGATGAGAGAAGAGCATGTGAAGAGCAAAAGTCAAGCAGCAGAA GGGCTGACAATGCGGTGCCCTGTGAGGCGAGACCAGCCAGGGGGTCCAGTCACCTGTCCTCTCGAACTGA GAGCAGTTTGAAATCTGATGGTTACCACAAGCGACCCGACCGCAAGTCCAGAATCCTTGCGAAGAGTGCA TCTACCTCTAAACCTGAATTTGAGTTTAGTAGGTTAGACTTTCCTGAACTGCAGAGTCCAAAGAACAGTA ACATGCCAGAGACACAGAAGCCGCCCAGGTGGGGGCCTCTTGGCCCTGCTGCCAGTAACATGCCTCTCCT AGGAGACGTCGGCAAGCCCGTCGCAGATATGGTAGAGGGCAAAATGGTGAAGAGCGATCACACTGATGGA GCTGTGACCAGTAATGCCACTACCAGTTCCCCTTCATGTACCCAAGAGTTGTCTTGGACACCAATGGGTT ATATTGTTCGGCAGACAGTGTCTTCAGATTCAGCAGCAGCCACTGAAAATGTGACTTCCATGATAAACCT AAAGAAGACTACTTCATCAGCTGATGCTAAAAATGTTAGTGTGACATCTGAGGCTTTATCTTCAAATCCT TCCTACAACAGAGAAAAGCGTGTTTATCCTGCTCCAAAGGCCAAAGCATCACAAGGAGGTGAACTTGAAC AAAACGAAAGCTCCAAAAAGAATAAGAAAAAGAAAGAGAAGTCTAAACCGAGTTATGAAGTCCTGACGGT TCAGGAGCCGCCAAGGATTGAAGATGCAGAGGAATTCCCCAACCTGTCAGTTGCGTCGGAAAGAAGACAC AGAGGGCAATCACCGAAGCTTCACAGTAAACAGCAGACGCAGAATGAATTTAAAACAAGTGGGAAGAAGA GCCAGGTCCCAGTGCAGCTGGACCTGGGGGGCATGCTGGCCGCGCTGGAGAAGCAGCAGCAGCAGCAGCA CGCCTCGCACGCCAAGCCATCCTCCAGACCCGTCGTGTTCTCAGTTGGAGCAGTGCCAGTCCTGTCCAAG GATGCCTCCTCCAGTGAGAGGGGACGCCGCTCCAGTCAGATGAAGACCCCACACAACCCCCTGGACTCCA GTGCCCCCCTGATGAAGAAGGGGAAGCAGAGGGAGATACCTAAGGCCAAGAAGCCCACCTCACTGAAGAA GATAATTTTGAAAGAACGGCAAGAGAGGATGCAGCAGCGACTCCAAGAAAGTGCTGTGAGCCTGACGGTG GCCAGTGATGACTCACAGGATGTGGAGAGTGGCGCCAGTAACCAAACCCCCAGTCAGGACAACCCCACAG GTCCAGAGAAGACAGAAGAATCAGTGTCTTCTACACCTGTGGTTGAGGGTGAGTCAGAGGAGCCAGCTGG CACAGAGTTCCAGAGGGACCCAGAGGCTTGCCAGCCTGCCCCTGACAGTGCCACCTTCCCCAAGATCCAC AGCCGGAGGTTCCGGGACTACTGCAGCCAGATGCTTAGTAAAGAAGTAGATGCTTGTGTCACGGGTCTGC TCAAGGAGCTGGTGCGCTTCCAAGACCGCATGTACCAGAAGGATCCCGTCAAGGCCAAGACAAAACGGCG GCTCGTGCTGGGGCTGAGGGAGGTCCTGAAACACCTGAAGCTCAGGAAGCTGAAGTGCATCATCATCTCT CCCAACTGTGAGAAGACCCAGTCTAAAGGTGGGCTGGACGACACGCTGCACACCATCATCGACTGCGCCT GCGAGCAGAACATCCCCTTCGTGTTTGCACTCAATCGCAAGGCTCTGGGGCGGAGCCTGAATAAAGCTGT TCCTGTCAGCATTGTAGGGATCTTCAGCTACGATGGGGCCCAGGACCAGTTCCACAAGATGGTTGAGCTG ACCATGGCAGCCCGTCAGGCATACAAGACCATGTTGGAGACAATGCGGCAGGAACAGGCAGGAGAACCTG GACCTCAGTCCCCTCCCAGCCCACCCATGCAAGACCCCATCCCATCCACGGAAGAAGGCACCCTCCCTTC CACTGGAGAAGAGCCACACTACATTGAGATTTGGAAAAAGCACCTGGAAGCGTACAGTCAGCGTGCCCTG GAGCTGGAAGACTCACTGGAGGCGTCAACCTCTCAGATGATGAACTTGAATTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221507 protein sequence
Red=Cloning site Green=Tags(s) MASERPREPDGEDSIKLSADVKPFVPKFAGLNVAWSESSETRVFPGCAATYYPFVQEPPAAEQKMYPEDM AFGAPTFPAQYVSSEIALHPFAYPTYTLESAQSVCSVPTLQYDYSQARCHPGFRTAKPRHEHVCPPPQEA KGVFKKKPSDERRACEEQKSSSRRADNAVPCEARPARGSSHLSSRTESSLKSDGYHKRPDRKSRILAKSA STSKPEFEFSRLDFPELQSPKNSNMPETQKPPRWGPLGPAASNMPLLGDVGKPVADMVEGKMVKSDHTDG AVTSNATTSSPSCTQELSWTPMGYIVRQTVSSDSAAATENVTSMINLKKTTSSADAKNVSVTSEALSSNP SYNREKRVYPAPKAKASQGGELEQNESSKKNKKKKEKSKPSYEVLTVQEPPRIEDAEEFPNLSVASERRH RGQSPKLHSKQQTQNEFKTSGKKSQVPVQLDLGGMLAALEKQQQQQHASHAKPSSRPVVFSVGAVPVLSK DASSSERGRRSSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERMQQRLQESAVSLTV ASDDSQDVESGASNQTPSQDNPTGPEKTEESVSSTPVVEGESEEPAGTEFQRDPEACQPAPDSATFPKIH SRRFRDYCSQMLSKEVDACVTGLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIIS PNCEKTQSKGGLDDTLHTIIDCACEQNIPFVFALNRKALGRSLNKAVPVSIVGIFSYDGAQDQFHKMVEL TMAARQAYKTMLETMRQEQAGEPGPQSPPSPPMQDPIPSTEEGTLPSTGEEPHYIEIWKKHLEAYSQRAL ELEDSLEASTSQMMNLNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029279 |
| ORF Size | 2577 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029279.2, NP_083555.1 |
| RefSeq Size | 2913 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 75420 |
| MW | 94.8 kDa |
| Gene Summary | The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in a similar gene in human have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222169 | Secisbp2 (untagged) - Mouse SECIS binding protein 2 (Secisbp2), (10ug) |
CNY 10,930.00 |
|
| MG221507 | Secisbp2 (tGFP-tagged) - Mouse SECIS binding protein 2 (Secisbp2), (10ug) |
CNY 7,890.00 |
|
| MR221507L3 | Lenti ORF clone of Secisbp2 (Myc-DDK-tagged) - Mouse SECIS binding protein 2 (Secisbp2) |
CNY 9,030.00 |
|
| MR221507L4 | Lenti ORF clone of Secisbp2 (mGFP-tagged) - Mouse SECIS binding protein 2 (Secisbp2) |
CNY 9,030.00 |
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