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Mlph (NM_053015) Mouse Tagged ORF Clone
CAT＃: MR221872
- TrueORF®
Mlph (Myc-DDK-tagged) - Mouse melanophilin (Mlph)
ORF Plasmid: tGFP
"NM_053015" in other vectors (4)
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CNY 4,392.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2210418F23Rik; 5031433I09Rik; AW228792; D1Wsu84e; l(1)-3Rk; l1Rk3; ln; Slac-2a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221872 representing NM_053015
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAAAGGTTGGACCTTTCCACGCTCACAGACGAGGAGGCTGAGCACGTGTGGGCAGTGGTTCAGC GGGACTTTGACCTCAGGAGGCGAGAGGAAGAAAGACTCCAGGGGCTGAAGGGCAAAATACAAAAGGAGAG CTCCAAGAGGGAGCTGCTGTCGGACACAGCCCATCTGAATGAGACTCACTGTGCCCGCTGCCTGCAGCCC TACCGGCTGCTCCTGAACAGCAGACGACAGTGCCTAGAGTGCAGCCTCTTCGTCTGCAAAAGCTGCAGCC ACGCCCACCCAGAAGAGCAGGGCTGGCTCTGCGACCCCTGCCACCTGGCCAGGGTCGTGAAGATCGGTTC TCTGGAGTGGTACTACCAGCACGTGAGGGCTCGCTTCAAGCGTTTCGGGAGTGCCAAAGTGATCCGGTCT CTCTGTGGGCGGCTGCAGGGTGGAGGTGGATCTGAGCCAAGCTTGGAAGAAGGAAATGGAGACAGTGAGC AGACTGATGAGGATGGAGACCTGGACACAGAGGCCAGAGACCAGCCCCTCAACAGCAAAAAGAAAAAGCG CCTGCTCTCCTTCCGAGATGTGGACTTTGAGGAAGACTCAGACCACTTGGTGCAGCCTTGCAGCCAAACC TTGGGCCTGTCCTCAGTCCCTGAGTCTGCACACAGCCTGCAGTCCCTCTCAGGTGAGCCCTACTCTGAGG ACACCACCTCTCTGGAGCCCGAGGGCCTAGAGGAGACTGGTGCAAGGGCTTTGGGATGTCATCCCAGTCC TGAAGTGCAGCCATGTAGCCCTTTACCCTCTGGGGAGGATGCTCACGCTGAACTGGACTCGCCTGCAGCA TCCTGCAAGAGTGCCTTTGGGACCACAGCTATGCCTGGAACAGACGATGTCAGGGGCAAACATCTGCCCT CACAGTACCTGGCTGATGTAGACACCTCTGATGAAGACAGTATCCAGGGTCCCAGGGCAGCCTCCCAGCA TAGCAAGAGGAGGGCCCGGACTGTGCCTGAGACTCAGATCTTGGAGTTGAACAAGCGAATGTCAGCTGTG GAGCACCTGCTAGTCCACCTGGAGAATACGGTTCTGCCACCCTCAGCCCAGGAACCAACTGTGGAGACAC ACCCCAGTGCTGACACAGAGGAGGAGACACTCAGGAGGAGGCTGGAGGAGCTGACCAGCAACATCAGTGG TTCCAGCACCTCATCAGAAGATGAGACCAAGCCAGATGGCACCTTCCTTGGAGGGTCCCCAAAGGTGTGC ACAGACACAGGGCACATGGAGACACAGGAAAGGAACCCTCGGAGCCCTGGGAACCCTGCTCGGCCTACAA AAAGCACAGATGAGGAGCTCTCTGAGATGGAGGATAGAGTGGCCATGACAGCCTCTGAAGTTCAGCAGGC TGAGAGTGAGATCTCAGACATCCAGTCCAGGATCGCAGCTCTGAGAGCCGCAGGACTCACAGTGAAGCCC TCGGGAAAACCTCGGAGAAAGTCAGGCATCCCGATCTTTCTTCCCCGCGTTACTGAGAAACTTGACAGGA TCCCAAAGACTCCACCTGCAGACCCTGATGACCAAGCCAAGATGCCCAAGGCAACAACAGCTGTGCCCTC TCTCCTGAGGAGGAAGTATTCTCCCAGCAGCCAAGGCGTAGACAGTGGTTCTTTTGATCGGAAATCAGTG TACCGTGGCTCGCTGACACAAAGGAACCCTAACGGGAGGAGAGGGACAGCCAGACACATCTTCGCGAAAC CCGTGATGGCCCAGCAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221872 representing NM_053015
Red=Cloning site Green=Tags(s) MGKRLDLSTLTDEEAEHVWAVVQRDFDLRRREEERLQGLKGKIQKESSKRELLSDTAHLNETHCARCLQP YRLLLNSRRQCLECSLFVCKSCSHAHPEEQGWLCDPCHLARVVKIGSLEWYYQHVRARFKRFGSAKVIRS LCGRLQGGGGSEPSLEEGNGDSEQTDEDGDLDTEARDQPLNSKKKKRLLSFRDVDFEEDSDHLVQPCSQT LGLSSVPESAHSLQSLSGEPYSEDTTSLEPEGLEETGARALGCHPSPEVQPCSPLPSGEDAHAELDSPAA SCKSAFGTTAMPGTDDVRGKHLPSQYLADVDTSDEDSIQGPRAASQHSKRRARTVPETQILELNKRMSAV EHLLVHLENTVLPPSAQEPTVETHPSADTEEETLRRRLEELTSNISGSSTSSEDETKPDGTFLGGSPKVC TDTGHMETQERNPRSPGNPARPTKSTDEELSEMEDRVAMTASEVQQAESEISDIQSRIAALRAAGLTVKP SGKPRRKSGIPIFLPRVTEKLDRIPKTPPADPDDQAKMPKATTAVPSLLRRKYSPSSQGVDSGSFDRKSV YRGSLTQRNPNGRRGTARHIFAKPVMAQQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053015 |
| ORF Size | 1770 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053015.3, NP_443748.2 |
| RefSeq Size | 4484 bp |
| RefSeq ORF | 1773 bp |
| Locus ID | 171531 |
| UniProt ID | Q91V27 |
| MW | 65.5 kDa |
| Gene Summary | Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219452 | Mlph (untagged) - Mouse melanophilin (Mlph), (10ug) |
CNY 7,510.00 |
|
| MG221872 | Mlph (tGFP-tagged) - Mouse melanophilin (Mlph), (10ug) |
CNY 5,800.00 |
|
| MR221872L3 | Lenti ORF clone of Mlph (Myc-DDK-tagged) - Mouse melanophilin (Mlph) |
CNY 7,130.00 |
|
| MR221872L4 | Lenti ORF clone of Mlph (mGFP-tagged) - Mouse melanophilin (Mlph) |
CNY 7,130.00 |
