Psmd4 (NM_008951) Mouse Tagged ORF Clone

CAT#: MR222032

  • TrueORF®

Psmd4 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (Psmd4)

ORF Plasmid: DDK tGFP



  "NM_008951" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Af1; angiocidin; Mcb1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR222032 representing NM_008951
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGTTGGAGAGCACTATGGTTTGTGTGGACAACAGTGAGTACATGCGGAACGGAGACTTCCTTCCCA
CCCGGCTGCAGGCCCAGCAGGATGCCGTCAACATTGTATGTCACTCAAAGACCCGAAGCAACCCTGAGAA
TAACGTGGGCCTGATCACACTGGCCAATGACTGTGAGGTGCTGACCACACTCACCCCGGACACTGGCCGT
ATCCTCTCCAAGCTCCACACTGTCCAACCCAAAGGCAAGATCACCTTCTGCACTGGCATCCGCGTGGCCC
ACTTGGCTCTGAAGCACCGGCAGGGCAAGAATCACAAGATGCGCATCATCGCCTTTGTCGGTAGCCCTGT
GGAGGACAACGAGAAGGATCTGGTGAAACTAGCTAAACGCCTTAAGAAAGAAAAAGTGAATGTTGACATC
ATTAATTTTGGGGAAGAGGAGGTGAACACAGAGAAGCTGACAGCCTTTGTGAACACACTGAATGGCAAGG
ATGGAACTGGGTCCCATCTAGTGACAGTGCCTCCTGGACCTAGCTTGGCTGATGCTCTCATCAGTTCTCC
TATTCTGGCTGGTGAAGGCGGTGCCATGCTGGGTCTTGGTGCCAGTGACTTTGAGTTTGGAGTAGATCCC
AGTGCTGATCCTGAATTGGCCCTGGCCCTTCGAGTCTCTATGGAAGAGCAGCGGCAGCGGCAGGAGGAAG
AGGCACGGCGGGCCGCTGCGGCCTCTGCAGCTGAGGCTGGAATTGCTACACCTGGGACTGAAGACTCGGA
TGACGCCCTACTGAAGATGACCATCAACCAGCAGGAGTTTGGCCGTCCTGGGCTTCCAGACCTAAGCAGC
ATGACTGAGGAAGAGCAGATCGCCTACGCCATGCAGATGTCCCTGCAGGGAACAGAGTTTAGCCAAGAAT
CGGCTGACATGGATGCCAGCTCAGCCATGGACACATCTGATCCAGTCAAGGAGGAGGATGACTATGACGT
CATGCAGGACCCGGAGTTCCTTCAGAGCGTCCTAGAGAACCTTCCAGGTGTGGATCCCAACAATGCAGCC
ATTCGAAGTGTCATGGGGGCTCTGGCCTCCCAGGCCACCAAGGATGGCAAGAATGACAAGAAAGAGGAAG
AGAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR222032 representing NM_008951
Red=Cloning site Green=Tags(s)

MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGR
ILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDI
INFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDP
SADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATPGTEDSDDALLKMTINQQEFGRPGLPDLSS
MTEEEQIAYAMQMSLQGTEFSQESADMDASSAMDTSDPVKEEDDYDVMQDPEFLQSVLENLPGVDPNNAA
IRSVMGALASQATKDGKNDKKEEEKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_008951
ORF Size 1128 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_008951.2
RefSeq Size 1252 bp
RefSeq ORF 1131 bp
Locus ID 19185
UniProt ID O35226
MW 41.2 kDa
Gene Summary Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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