Epm2a (NM_010146) Mouse Tagged ORF Clone
CAT#: MR222730
- TrueORF®
Epm2a (Myc-DDK-tagged) - Mouse epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a)
ORF Plasmid: tGFP
"NM_010146" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | TcrbK)TG-BFlv; Tg(TcraK; TG-B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222730 representing NM_010146
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTTCCGCTTCGGCGTGGTGGTGCCGCCCGCGGTGGCCGGCGCTCGGCAGGAGCTGCTGCTCGCCG GCTCGCGGCCCGAGTTAGGGCGCTGGGAGCCGCACGGCGCCGTGCGTCTCAGGCCCGCGGGTACCGCGGC GGGCGCCGCTGCGCTGGCGCTGCAGGAGCCCGGCCTGTGGCTCGCCGAGGTGGAGCTGGAGGCGTACGAG GAGGCCGGCGGGGCGGAGCCGGGCCGCGTTGACACGTTCTGGTACAAGTTCCTGCAGCGCGAGCCTGGAG GCGAGCTGCACTGGGAAGGAAATGGACCTCACCATGACCGTTGCTGCACATATAATGAGGACAACTTGGT GGATGGTGTTTATTGTCTCCCAGTAGGACACTGGATTGAGGCCACTGGGCACACCAATGAAATGAAGCAC ACAACAGACTTCTATTTTAATATTGCTGGCCACCAAGCCATGCACTATTCAAGAATTCTACCAAATATCT GGCTGGGTAGCTGCCCTCGCCAACTGGAACATGTGACCATCAAACTGAAGCATGAACTGGGAGTTACAGC TGTCATGAATTTCCAGACTGAATGGGATATCATCCAGAATTCTTCAGGCTGCAACCGCTACCCTGAACCC ATGACTCCAGACACCATGATGAAGCTGTATAAGGAAGAAGGCTTGTCCTACATCTGGATGCCCACTCCAG ACATGAGCACTGAGGGCCGAGTGCAGATGCTGCCACAGGCTGTGTGTCTCCTGCACGCGCTTCTGGAGAA TGGACACACGGTGTATGTCCACTGCAACGCTGGCGTGGGTCGCTCCACAGCTGCAGTGTGCGGCTGGCTC CACTATGTGATTGGCTGGAATCTGCGCAAGGTGCAGTACTTCATCATGGCCAAGAGGCCTGCGGTCTACA TTGACGAGGACGCTTTGGCTCAAGCACAACAAGACTTTTCTCAGAAGTTCGGGAAGGTTCACTCTTCCAT ATGCGCTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222730 representing NM_010146
Red=Cloning site Green=Tags(s) MLFRFGVVVPPAVAGARQELLLAGSRPELGRWEPHGAVRLRPAGTAAGAAALALQEPGLWLAEVELEAYE EAGGAEPGRVDTFWYKFLQREPGGELHWEGNGPHHDRCCTYNEDNLVDGVYCLPVGHWIEATGHTNEMKH TTDFYFNIAGHQAMHYSRILPNIWLGSCPRQLEHVTIKLKHELGVTAVMNFQTEWDIIQNSSGCNRYPEP MTPDTMMKLYKEEGLSYIWMPTPDMSTEGRVQMLPQAVCLLHALLENGHTVYVHCNAGVGRSTAAVCGWL HYVIGWNLRKVQYFIMAKRPAVYIDEDALAQAQQDFSQKFGKVHSSICAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010146 |
ORF Size | 990 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010146.2, NP_034276.2 |
RefSeq Size | 1079 bp |
RefSeq ORF | 993 bp |
Locus ID | 13853 |
UniProt ID | Q9WUA5 |
MW | 37.4 kDa |
Gene Summary | Plays an important role in preventing glycogen hyperphosphorylation and the formation of insoluble aggregates, via its activity as glycogen phosphatase, and by promoting the ubiquitination of proteins involved in glycogen metabolism via its interaction with the E3 ubiquitin ligase NHLRC1/malin (PubMed:18040046, PubMed:18852261, PubMed:19036738, PubMed:23663739, PubMed:24430976, PubMed:24068615). Dephosphorylates phosphotyrosine and synthetic substrates, such as para-nitrophenylphosphate (pNPP), and has low activity with phosphoserine and phosphothreonine substrates (in vitro) (PubMed:16971387, PubMed:24430976). Has also been shown to dephosphorylate MAPT (PubMed:19542233). Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates (PubMed:18040046, PubMed:18852261, PubMed:23663739). Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS) (PubMed:19036738, PubMed:24068615). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Also promotes proteasome-independent protein degradation through the macroautophagy pathway (PubMed:20453062).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208444 | Epm2a (untagged) - Mouse epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a), (10ug) |
CNY 3,990.00 |
|
MG222730 | Epm2a (tGFP-tagged) - Mouse epilepsy progressive myoclonic epilepsy type 2 gene alpha (Epm2a), (10ug) |
CNY 2,850.00 |
|
MR222730L3 | Lenti ORF clone of Epm2a (Myc-DDK-tagged) - Mouse epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a) |
CNY 4,750.00 |
|
MR222730L4 | Lenti ORF clone of Epm2a (mGFP-tagged) - Mouse epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a) |
CNY 4,750.00 |