Zcchc8 (NM_027494) Mouse Tagged ORF Clone
CAT#: MR222992
- TrueORF®
Zcchc8 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 8 (Zcchc8)
ORF Plasmid: tGFP
"NM_027494" in other vectors (3)
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CNY 7,030.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 5730565F05Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222992 representing NM_027494
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGGTGTGGATTTTGGCGACCTAGAGCTCTTCGAGGCGTTTGACCCACCAGAGGAGTCGACGC CGAAGCCCGTTCACACCCGGTTCAAGGACGACGAGGAGGAAGAGGACGACGACGACGACGAGAACGGGGT GGGCGATGCGGAGCTGCAGGAGCAGCTCCGGCGCTGCGAGGCCACCATCGAGCAGCTCCGCGCCGAGAAT CAAGAACTTAAAAGAAAGTTGAACATTCTGACAAGACCCAGTGGTATACTGGTGAGCAATACTAAGATAG ATGGACCGTTACTACAAATTCTATTTATGAACAATGCTATCTCAAAGCAATACCACCAAGAAATAGAGGA GTTTGTATCAAACTTAGTAAAACGATTTGAGGAACAGCAAAAAAATGACGTGGAAAAGACTTCCTTCAGC CTTTTACCCCAGCCGTCCAGTGTTATGTTGGAAGAGGACCATAAAGTGGAAGAATCATGTGCCGTTAAAA ACAACAAGGAAGCTTTTAGTGTTGTAGGAAGTGTCCTGTATTTTACTAATTTTTGCCTTGATAAATTGGG GCAACCGCTACTAAATGAAAACCCTCAGCTTACGGAAGGATGGGAAATACCCAAGTACCAGCAAGTCTTC AGCCACATTGTTCCTCTAGAAGGGCAAGAGATGCAAGTGAAGGCAAAAAGGCCAAAGCCTCACTGTTTCA ACTGTGGTTCTGAAGAGCATCAGATGAAGGAATGCCCAATGCCTCGGAATGCTGCTCGGATCAGTGAGAA GAGGAAAGAGTACATGGATGCCTGTGGCGAGGCCAGCGGCCAGAGCTTCCAGCAGCGCTATCATGCCGAA GAAGTAGAAGAGAGATTTGGAAGATTCAAGCCAGGAGTTATTAGTGAGGAACTTCAAGATGCACTGGGTG TGACAGACAAGAGTCTTCCCCCTTTCATCTACCGGATGCGCCAGCTGGGCTACCCACCGGGCTGGCTCAA GGAGGCTGAACTGGAGAATTCTGGACTTGCACTCTATGATGGAAACGATGACGCTGATGGGGAAACAGAA ACTGGAGAAATACAGAATAAAAATGTCACCTACGATCTCTCAAAATTGGTAAACTATCCAGGTTTTAATA TATCTACTCCCAGAGGTATTCCAGATGAATGGAGAATGTTCGGTTCCATACCAATGCAGGCGTGTCAACA GAAGGATGTGTTTGCCAGTTACCTTAATTCTAACATCCAGTCGCCGAGTATGAGATCTAGCGGCAAGCGG TCTTCCTCGCAGTCCAGCCCTAACAGTCCAAAGAAGCAGCGAAAGGAGGGCAGTGCGGCGGCCTCCCCTG CCGACATGGAGCTAGACTCAGATGTAGAGATTCCACCTGGTTCTCAGAGCAGCAAAGCATTTCAGTTCCA ACCACCGTTGCCTCCCGGAACACCGCCTCCACTCCCACAGGGAACTCCCCCGCCTCTCTTCACCCCTCCA CTCCCTAAGGGAACCCCACCACTGACTCCCAGTGACTCACCCCAGGCCCGACCCGCAGCCTCAGCCATGG ATGAGGATGCCCTGACGCTGGAGGAGCTGGAAGAGCAGCAGAGGCAGATCTGGGCGGCTCTCCAGCAGGC CGAGGGAGGGAATGGCGACTCAGATGTTCCTGGAGACACACCTTTAACTGGGAACTCAGTGGCCTCCTCC CCGTGTCCAAACGAGTTTGACCTCCCTGTCCCCGAAGGAAAGGCCCTGGAAAAGCCAGTGCTGGCTGAGC CCCAGGAACCAGCTGCTTCTGTAGACACAGCTGGACCCGAGCCATCCTGCAGCCCAGCAGCAGGGGCAGC AGTGCTCTCTCAGAGAGAAGAGGAAGCTGCTGCTGAGGGGGGCCCGGGAGATGCTCTTCTCGACAATGGC GGTGTGTTGAACATGAACATGAGCAATGGGAGCAACCAGCAGCCCATTCACCCGGACAGCCGGCCTCCGA TGGCCCCAAAAACCCATAGCCCAGTACCTGACATGAGCAAGTTTGCCACTGGAATAACACCCTTTGAATT TGAGAACATGGCTGAATCCACTGGAATGTACCTCAGGATAAGAAACCTGTTGAAGAATTCACCCCGAAAT CAGCAGAAAAACAAAAAGACTTGTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222992 representing NM_027494
Red=Cloning site Green=Tags(s) MAAGVDFGDLELFEAFDPPEESTPKPVHTRFKDDEEEEDDDDDENGVGDAELQEQLRRCEATIEQLRAEN QELKRKLNILTRPSGILVSNTKIDGPLLQILFMNNAISKQYHQEIEEFVSNLVKRFEEQQKNDVEKTSFS LLPQPSSVMLEEDHKVEESCAVKNNKEAFSVVGSVLYFTNFCLDKLGQPLLNENPQLTEGWEIPKYQQVF SHIVPLEGQEMQVKAKRPKPHCFNCGSEEHQMKECPMPRNAARISEKRKEYMDACGEASGQSFQQRYHAE EVEERFGRFKPGVISEELQDALGVTDKSLPPFIYRMRQLGYPPGWLKEAELENSGLALYDGNDDADGETE TGEIQNKNVTYDLSKLVNYPGFNISTPRGIPDEWRMFGSIPMQACQQKDVFASYLNSNIQSPSMRSSGKR SSSQSSPNSPKKQRKEGSAAASPADMELDSDVEIPPGSQSSKAFQFQPPLPPGTPPPLPQGTPPPLFTPP LPKGTPPLTPSDSPQARPAASAMDEDALTLEELEEQQRQIWAALQQAEGGNGDSDVPGDTPLTGNSVASS PCPNEFDLPVPEGKALEKPVLAEPQEPAASVDTAGPEPSCSPAAGAAVLSQREEEAAAEGGPGDALLDNG GVLNMNMSNGSNQQPIHPDSRPPMAPKTHSPVPDMSKFATGITPFEFENMAESTGMYLRIRNLLKNSPRN QQKNKKTCE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_027494 |
ORF Size | 2127 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027494.3, NP_081770.3 |
RefSeq Size | 4286 bp |
RefSeq ORF | 2130 bp |
Locus ID | 70650 |
UniProt ID | Q9CYA6 |
MW | 78.5 kDa |
Gene Summary | Scaffolding subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters. May be involved in pre-mRNA splicing.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG222992 | Zcchc8 (tGFP-tagged) - Mouse zinc finger CCHC domain containing 8 (Zcchc8), (10ug) |
CNY 7,700.00 |
|
MR222992L3 | Lenti ORF clone of Zcchc8 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 8 (Zcchc8) |
CNY 8,930.00 |
|
MR222992L4 | Lenti ORF clone of Zcchc8 (mGFP-tagged) - Mouse zinc finger, CCHC domain containing 8 (Zcchc8) |
CNY 8,930.00 |