Phf21a (NM_001109691) Mouse Tagged ORF Clone
CAT#: MR223342
- TrueORF®
Phf21a (Myc-DDK-tagged) - Mouse PHD finger protein 21A (Phf21a), transcript variant 3
ORF Plasmid: tGFP
"NM_001109691" in other vectors (3)
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CNY 5,990.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 80kDa; Bhc; Bhc80; D030065N23Rik; PFTF1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223342 representing NM_001109691
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGCAGACTCTACAGGAGGCTCTTAAAGTGGAAATTCAGGTTCACCAGAAACTGGTTGCCCAAA TGAAGCAGGATCCACAGAATGCTGACTTAAAGAAACAGCTTCATGAACTCCAAGCCAAAATCACAGCTTT GAGTGAGAAACAGAAAAGAGTAGTTGAGCAGCTGCGCAAGAACCTGATAGTCAAACAAGAGCAGCCGGAC AAGTTCCAGATACAGCCATTGTCACAGTCTGAAAACAAACTACAAACAGCACAGCAGCAGCCACTACAGC CACTGCAGCAGCAGCAACCGCAGCAACCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACGCCCAGCAGTC AGCAGCAGCACCGCCTAGTCTAACTGCATCACAGCAGGTTCAAGCAACACCTCCTCAGCCCATCAAAGTA CCACAGTTTATCCCTCCTCCTAGACTCACTCCACGCCCAAACTTCCTTCCACAGGTTCGACCCAAGCCTG TGGCCCAGAATAACATTCCTATCGCTCCAGCGCCTCCTCCCATGCTTGCAGCTCCTCAGCTTATCCAGAG GCCTGTCATGCTGACCAAGTTTACACCCACAACCCTCCCCACATCCCAGAATTCCATCCACCCTGTCCGT GTTGTCAATGGACAGACTGCAACCATAGCCAAAACGTTCCCCATGGCCCAGCTCACCAGCATTGTCATAG CTACTCCAGGGACCAGACTCCCTGGACCTCAGACTGTACAGCTTAGCAAACCAAGCCTGGAGAAACAGAC AGTTAAATCCCACCCAGAAGCAGAGGAAAAACAAGCAGAGAGCCGTACCGTTACCCCACCTGCTGCACCA AAACCAAAACGAGAAGAGAACCCTCAGAAACTTGCCTTCATGGTGTCTCTAGGGTTGGTAACACATGACC ATCTAGAAGAAATCCAAAGCAAGAGGCAGGAAAGAAAGAGAAGAACAACAGCAAACCCGGTGTACAGTGG GGCTGTCTTTGAGCCAGAGCGTAAGAAGAGTGCAGTCACTTACCTTAACAGCACAATGCATCCTGGGACG CGGAAGAGAGGTCGTCCTCCAAAATACAATGCGGTGCTGGGGTTTGGAGCCCTTACCCCAACATCCCCCC CATCCAGTCATCCCGACTCCCCTGAAAATGAAAAGACAGAGACCACATTCACTTTCCCTGCACCTGTTCA GCCTGTGTCTTTGCCCAGCCCCACCTCCACAGACGGTGATATCCATGAGGATTTTTGCAGTGTTTGCAGA AAAAGTGGCCAGTTGCTGATGTGTGACACGTGTTCCCGTGTGTATCATCTGGACTGCTTAGAGCCGCCCC TGAAAACAATTCCCAAGGGCATGTGGATCTGTCCCAGATGTCAGGACCAGATGCTAAAGAAGGAAGAAGC AATTCCTTGGCCTGGAACCTTAGCCATTGTTCATTCCTACATCGCCTACAAAGCAGCAAAAGAAGAGGAG AAACAGAAGCTACTTAAGTGGAGTTCAGATTTAAAACAGGAGCGGGAACAACTGGAGCAGAAGGTCAAAG AGCTCAGCAGTTCTATAAGTAAATGTATGGAGATGAAGAGCAGCATCCTGGCGCGGCAGAAGGAGATGCG CAGCTCCCTGGACAAGGTGAAGCGGCTCATCCGCCTTGTGCATGGCGTCGACCTCTGCAGACCTGTGGAC TCAGAGGCCACGGCGGGGGCCCTCTCCAATGGCCCAGACTGCACTCCCCCTGCCAACGCCGCCTCCACAC CCGCCCCTTCCCCCTCCTCGCAGAGCTGCACAGCGAACTGTAACCAGGGGGAAGAGACTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223342 representing NM_001109691
Red=Cloning site Green=Tags(s) MELQTLQEALKVEIQVHQKLVAQMKQDPQNADLKKQLHELQAKITALSEKQKRVVEQLRKNLIVKQEQPD KFQIQPLSQSENKLQTAQQQPLQPLQQQQPQQPQQQQQQQQQHAQQSAAAPPSLTASQQVQATPPQPIKV PQFIPPPRLTPRPNFLPQVRPKPVAQNNIPIAPAPPPMLAAPQLIQRPVMLTKFTPTTLPTSQNSIHPVR VVNGQTATIAKTFPMAQLTSIVIATPGTRLPGPQTVQLSKPSLEKQTVKSHPEAEEKQAESRTVTPPAAP KPKREENPQKLAFMVSLGLVTHDHLEEIQSKRQERKRRTTANPVYSGAVFEPERKKSAVTYLNSTMHPGT RKRGRPPKYNAVLGFGALTPTSPPSSHPDSPENEKTETTFTFPAPVQPVSLPSPTSTDGDIHEDFCSVCR KSGQLLMCDTCSRVYHLDCLEPPLKTIPKGMWICPRCQDQMLKKEEAIPWPGTLAIVHSYIAYKAAKEEE KQKLLKWSSDLKQEREQLEQKVKELSSSISKCMEMKSSILARQKEMRSSLDKVKRLIRLVHGVDLCRPVD SEATAGALSNGPDCTPPANAASTPAPSPSSQSCTANCNQGEETK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001109691 |
ORF Size | 1812 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001109691.1, NP_001103161.1 |
RefSeq Size | 6484 bp |
RefSeq ORF | 1815 bp |
Locus ID | 192285 |
UniProt ID | Q6ZPK0 |
MW | 67.5 kDa |
Gene Summary | Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG223342 | Phf21a (tGFP-tagged) - Mouse PHD finger protein 21A (Phf21a) transcript variant 3, (10ug) |
CNY 6,560.00 |
|
MR223342L3 | Lenti ORF clone of Phf21a (Myc-DDK-tagged) - Mouse PHD finger protein 21A (Phf21a), transcript variant 3 |
CNY 7,890.00 |
|
MR223342L4 | Lenti ORF clone of Phf21a (mGFP-tagged) - Mouse PHD finger protein 21A (Phf21a), transcript variant 3 |
CNY 7,890.00 |