Glmn (NM_001161738) Mouse Tagged ORF Clone
CAT#: MR223429
- TrueORF®
Glmn (Myc-DDK-tagged) - Mouse glomulin, FKBP associated protein (Glmn), transcript variant 2
ORF Plasmid: tGFP
"NM_001161738" in other vectors (3)
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CNY 4,440.00
CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 9330160J16Rik; AW227515; Fap48; Fap68 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223429 representing NM_001161738
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGAGGAACTTCAGAGCATAATAAAAAGATGTCAAATCCTAGAAGAGCATGATTTTAAAGAAG AAGATTTTGGCCTCTTTCAGTTAGCAGGTCAAAGATGCATTGAAGATGGTTATATAAACCAGCTGCTAGA GATTATTCAAGATGAAAAGAACAAGACCATCATTAAGTCTATGGGGTGGAATCTTGTTGGTCCAGTTGTT CGATGCCTCCTGAGGGGCAGAGAAGAGGATAAAAGAGAAGAGTGTTTTCTGATATTTGATTTGCTGGTGA AGTTATGTAATCCAAAGGAATTGTTGTTGGGTTTGCTTGAACTCATTGAAGAGCCCTCCGGAAAACAGAT TTCCCAAATTATTCTTCTTTTACTGCAACCATTACAAACAGTTATTCAGAAACTTCCTAACAACAAGGCA TACTCCGTTGGACTAGCATTGTCAACACTTTGGAGTCAGCTGTCTCTTCTTCCTGTTCCACACTCAGAAG AACAAATTCAGGCAGATGATTATGGCCTCTGTCAGTGTTGTAAGGCCTTGATAGAGTTCACGAAACCTTT TGTGGAAGAAGTAATAAGTGATAAAGAAAACAAAGAAAATGCAAAACTAAAAGATGAATTACTGAAATTT TGTTTCAAAGGCTTGAAATGCCCTTTGCTGACAGCACAATTCCTCGAACAGTCAGAAGACGTTGGAAATG ACCCTTTTCGGTGTTTTGCATCTGAAATAATAGGATTTTTATCAAAAATTGGACACCCTGTCCCCCAAAT TATTCTTAATCATGGAAGGAAAAAAAGGACTTGGGATTACCTTGAATTTGAAGAAGAAGAAGACAAACAA CTGGCAGAGTCTGTGGCTTCTCTGACATATCTAGTATTTGTTCAGGGCATCGGTATTGATCAGCTGCCCA TGGTCTTAAGCCCATCGTACCTTCTGCAGTTGAACATGGAGCATATTGAAGTGTTTCTGCAAAGAACAGA ACAGTCTATTTACTCCAAAGGATTGGAACTTCTGGAGACTAGCTTATTGAGATTAGAAGACAACAGCCTA TGTTATCAGTACTTAGAAATCAAGAGTTTTCTTGCTGTGCCTCAGGGCTTAGTCAAAGTTATGACACTTT GCCCCATTGAGACATTGAGGAAAAAAGGTTTATCTATGCTTCAGCTGTTTATTGACAAGTTGGATTCACA AGGCAAATATACATTATTTAGGTGCTTACTAAATACAAGTAATCACTCAGGAGTGGAAGCCTTTGTAATT CAAAACATCAAAAATCAGATTGATTTATCATTTAAGAAAACATATAACAAATGGTTTGCAGGAGCACAGC TGATCTCTCTGTTAGACCTGGTCCTGTCTCTCCCTGAGGGCGCTGAGACAGACTTACTGCAGAACTCAGA CAGGATTATGGCTTCATTAAATTTATTGAGGTATTTGGTTATCAAAGATAATGAAGATGACAATCAAACT GGATTATGGACAGAACTTGGAAAAATTGAAAATAACTTTCTAAAGCCACTTCATATAGGACTTAATATGT CAAAAGCACATTATGAAGCAGAAATTAAAAACAGCCAACAAAATAACCAAGTAGCCTCAATGTGTAAAGG TGTTTGTTCCGTGACTGTAGGTGGAGAAGAAATCCCTTCTATGCCTCCTGAAATGCAGCTTAAGGTCTTA CATTCCGCTCTCTTCACATTTGACTTGATTGAAAGTGTTCTGGCTCGAGTAGAAGAACTCATTGAAATCA AATCAAAGTCTACCTCTGAAGAAAATGTTGGGATAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223429 representing NM_001161738
Red=Cloning site Green=Tags(s) MAVEELQSIIKRCQILEEHDFKEEDFGLFQLAGQRCIEDGYINQLLEIIQDEKNKTIIKSMGWNLVGPVV RCLLRGREEDKREECFLIFDLLVKLCNPKELLLGLLELIEEPSGKQISQIILLLLQPLQTVIQKLPNNKA YSVGLALSTLWSQLSLLPVPHSEEQIQADDYGLCQCCKALIEFTKPFVEEVISDKENKENAKLKDELLKF CFKGLKCPLLTAQFLEQSEDVGNDPFRCFASEIIGFLSKIGHPVPQIILNHGRKKRTWDYLEFEEEEDKQ LAESVASLTYLVFVQGIGIDQLPMVLSPSYLLQLNMEHIEVFLQRTEQSIYSKGLELLETSLLRLEDNSL CYQYLEIKSFLAVPQGLVKVMTLCPIETLRKKGLSMLQLFIDKLDSQGKYTLFRCLLNTSNHSGVEAFVI QNIKNQIDLSFKKTYNKWFAGAQLISLLDLVLSLPEGAETDLLQNSDRIMASLNLLRYLVIKDNEDDNQT GLWTELGKIENNFLKPLHIGLNMSKAHYEAEIKNSQQNNQVASMCKGVCSVTVGGEEIPSMPPEMQLKVL HSALFTFDLIESVLARVEELIEIKSKSTSEENVGIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001161738 |
ORF Size | 1788 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001161738.1, NP_001155210.1 |
RefSeq Size | 2001 bp |
RefSeq ORF | 1791 bp |
Locus ID | 170823 |
UniProt ID | Q8BZM1 |
MW | 67.8 kDa |
Gene Summary | Regulatory component of cullin-RING-based SCF (SKP1-Cullin-F-box protein) E3 ubiquitin-protein ligase complexes. Inhibits E3 ubiquitin ligase activity by binding to the RING domain of RBX1 and inhibiting its interaction with the E2 ubiquitin-conjugating enzyme CDC34. Inhibits RBX1-mediated neddylation of CUL1 (By similarity). Required for normal stability and normal cellular levels of key components of SCF ubiquitin ligase complexes, including FBXW7, RBX1, CUL1, CUL2, CUL3, CUL4A, and thereby contributes to the regulation of CCNE1 and MYC levels (PubMed:22405651). Essential for normal development of the vasculature (PubMed:22405651). Contributes to the regulation of RPS6KB1 phosphorylation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG223429 | Glmn (tGFP-tagged) - Mouse glomulin FKBP associated protein (Glmn) transcript variant 2, (10ug) |
CNY 5,040.00 |
|
MR223429L3 | Lenti ORF clone of Glmn (Myc-DDK-tagged) - Mouse glomulin, FKBP associated protein (Glmn), transcript variant 2 |
CNY 6,460.00 |
|
MR223429L4 | Lenti ORF clone of Glmn (mGFP-tagged) - Mouse glomulin, FKBP associated protein (Glmn), transcript variant 2 |
CNY 6,460.00 |