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Grm1 (NM_016976) Mouse Tagged ORF Clone
CAT＃: MR223498
- TrueORF®
Grm1 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 1 (Grm1), transcript variant 1
ORF Plasmid: tGFP
"NM_016976" in other vectors (4)
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CNY 8,352.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930455H15Rik; ENSMUSG00000075319; Gm10828; Gprc1a; mGluR1; nmf373; rcw; wobl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223498 representing NM_016976
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGGCTCCTCTTGATTTTCTTCCCAATGATCTTTTTGGAGATGTCCATTTTGCCCAGGATGCCTG ACAGAAAAGTACTGCTGGCAGGTGCCTCGTCCCAGCGCTCTGTGGCGAGAATGGACGGAGATGTCATCAT CGGAGCCCTCTTCTCAGTCCATCACCAGCCTCCAGCCGAAAAGGTACCGGAAAGGAAGTGTGGGGAGATC AGAGAACAGTATGGTATCCAGAGAGTGGAGGCCATGTTCCACACATTAGATAAGATTAATGCGGACCCGG TGCTCCTGCCCAACATCACTCTGGGCAGTGAGATCCGGGACTCCTGCTGGCACTCTTCTGTGGCTCTGGA ACAGAGCATTGAGTTCATCAGAGACTCCCTGATTTCCATCCGAGATGAGAAGGATGGACTGAACCGCTGC CTGCCTGATGGCCAGACCCTACCCCCAGGCAGGACTAAGAAGCCTATTGCTGGAGTGATCGGCCCTGGCT CCAGCTCTGTGGCCATTCAAGTCCAGAATCTTCTGCAGCTGTTCGACATCCCACAAATCGCCTATTCTGC CACGAGCATAGACCTGAGCGACAAAACTTTGTACAAATACTTCCTGAGGGTGGTCCCTTCTGACACTTTG CAGGCAAGGGCGATGCTTGATATCGTCAAGCGGTACAACTGGACCTATGTCTCTGCAGTCCACACAGAAG GGAATTACGGCGAGAGTGGAATGGATGCTTTCAAAGAGTTGGCTGCCCAGGAAGGACTCTGCATCGCACA CTCGGACAAAATCTACAGCAATGCTGGCGAGAAGAGCTTTGATCGGCTTCTGCGCAAGCTCCGAGAGCGG CTTCCCAAGGCCAGGGTTGTGGTCTGCTTCTGCGAGGGCATGACAGTGCGGGGGTTACTGAGTGCCATGC GCCGCCTTGGCGTCGTGGGAGAGTTCTCACTCATTGGAAGTGATGGATGGGCAGACAGAGATGAAGTCAT CGAAGGCTATGAGGTGGAAGCCAATGGGGGAATCACAATAAAGCTGCAGTCTCCAGAGGTCAGGTCGTTT GATGACTACTTTCTGAAGCTGAGGCTGGACACCAACACGAGGAATCCTTGGTTCCCTGAGTTCTGGCAAC ATCGCTTCCAGTGTCGCCTACCTGGACACCTCTTGGAAAACCCCAACTTTAAAAAAGTCTGCACAGGAAA TGAAAGCTTGGAAGAAAATTATGTCCAGGACAGCAAAATGGGATTTGTCATCAACGCCATCTATGCCATG GCACACGGCCTACAGAACATGCACCATGCTCTATGTCCTGGCTACGTGGGCCTTTGTGATGCCATGAAGC CCATTGACGGCAGGAAGCTCCTGGATTTCCTCATCAAATCCTCTTTTGTTGGAGTGTCTGGAGAGGAGGT CTGGTTCGATGAGAAGGGGGATGCACCTGGAAGGTATGACATTATGAATCTGCAGTACACAGAGGCTAAT CGCTATGACTATGTCCATGTGGGAACCTGGCATGAAGGTGTGCTGAATATCGATGATTACAAAATCCAGA TGAACAAAAGCGGAATGGTACGATCTGTGTGCAGCGAGCCTTGCTTAAAGGGTCAGATTAAGGTCATACG GAAAGGGGAAGTGAGCTGCTGCTGGATCTGCACAGCCTGCAAAGAGAATGAGTTTGTGCAAGATGAGTTT ACCTGCAGAGCCTGTGACCTGGGGTGGTGGCCCAATGCAGAGCTCACAGGCTGTGAGCCCATTACTATCC GTTACCTCGAGTGGAGTGACATAGAATCCATCATAGCCATCGCCTTTTCTTGCCTGGGCATCCTCGTGAC GCTATTTGTCACCCTCATCTTTGTGCTGTACCGGGACACACCTGTGGTCAAATCCTCCAGTAGAGAGCTC TGCTATATCATTCTGGCTGGTATTTTCCTCGGCTACGTATGCCCTTTCACCCTCATCGCCAAACCTACTA CCACATCCTGCTACCTCCAGCGCCTCCTAGTTGGCCTCTCTTCTGCCATGTGCTACTCTGCTCTTGTGAC CAAAACCAATCGTATTGCACGCATCCTGGCTGGCAGCAAGAAGAAGATCTGTACCCGGAAGCCCAGGTTC ATGAGCGCTTGGGCCCAAGTGATCATAGCCTCCATTCTGATTAGTGTACAGCTGACACTAGTGGTGACCT TGATCATCATGGAGCCTCCCATGCCCATTTTGTCCTACCCGAGCATCAAGGAAGTCTATCTTATCTGCAA TACCAGCAACCTGGGTGTAGTGGCACCTGTGGGTTACAACGGACTTCTCATCATGAGCTGTACCTACTAT GCCTTCAAGACCCGCAACGTGCCGGCCAATTTCAATGAGGCTAAATACATCGCCTTCACTATGTACACCA CCTGCATCATCTGGCTGGCTTTTGTTCCCATTTACTTTGGGAGCAACTACAAGATTATCACTACCTGCTT CGCAGTGAGCCTCAGTGTGACGGTGGCCCTGGGCTGCATGTTCACTCCCAAGATGTACATCATTATTGCC AAACCCGAGAGGAATGTCCGCAGTGCCTTCACCACCTCTGATGTAGTGCGCATGCACGTCGGTGACGGCA AGCTGCCATGCCGCTCCAACACCTTCCTCAACATTTTCCGGAGAAAGAAGCCTGGGGCAGGGAACGCCAA TTCTAACGGCAAGTCTGTGTCATGGTCTGAACCAGGTGGAAGACAGGCGCCCAAGGGACAGCATGTGTGG CAGCGCCTCTCTGTGCACGTGAAGACCAATGAGACGGCCTGTAACCAAACAGCCGTAATTAAACCCCTCA CTAAAAGTTACCAAGGCTCTGGCAAGAGTCTGACCTTTTCAGACGCCAGCACCAAGACCCTTTACAACGT GGAGGAAGAGGACAATACCCCTTCAACTCACTTCAGCCCTCCCAGCAGCCCTTCCATGGTGGTGCACCGA CGCGGGCCACCCGTGGCCACCACGCCACCTCTGCCACCCCACCTTAGCGCAGAAGAGACGCCCTTGTTCC TGGCTGATTCTGTCATCCCCAAGGGCTTGCCTCCTCCTCTCCCGCAGCAGCAACAGCAGCCGCCCCCTCA GCCGCCTCCGCAGCAGCCCAAATCCCTGATGGACCAGCTGCAAGGCGTGGTCACCAACTTTGGCTCTGGG ATTCCAGACTTCCACGCGGTGCTGGCAGGCCCGGGGACACCAGGGAACGGGCTGCGCTCCCTGTACCCGC CCCCGCCTCCACCGCAACACCTGCAGATGTTGCCCCTGCAGCTGAGCACCTTCCGAGAGGAGCCCATCTC CCCTCCGGGGGAGGACGACGATGATGACAGCAGCGAGAGGTTCAAGCTCCTGCAGGAGTTCGTGTATGAG CGTGAAGGGAACACGGAGGAAGACGATCTGGAAGAGGAGGAGGACCTGCCTGCAGCCAGCAAGCTGACGC CTGAGGATTCGCCTGCCCTGACGCCCCCTTCTCCTTTCCGAGATTCCGTGGCCTCTGGCAGCTCAGTGCC CAGTTCCCCAGTGTCTGAGTCAGTGCTCTGCACCCCTCCAAATGTGACCTATGCCTCTGTCATTCTGAGG GACTACAAGCAAAGCTCTTCCACTCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223498 representing NM_016976
Red=Cloning site Green=Tags(s) MVRLLLIFFPMIFLEMSILPRMPDRKVLLAGASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGEI REQYGIQRVEAMFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGLNRC LPDGQTLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDTL QARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRKLRER LPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEANGGITIKLQSPEVRSF DDYFLKLRLDTNTRNPWFPEFWQHRFQCRLPGHLLENPNFKKVCTGNESLEENYVQDSKMGFVINAIYAM AHGLQNMHHALCPGYVGLCDAMKPIDGRKLLDFLIKSSFVGVSGEEVWFDEKGDAPGRYDIMNLQYTEAN RYDYVHVGTWHEGVLNIDDYKIQMNKSGMVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEFVQDEF TCRACDLGWWPNAELTGCEPITIRYLEWSDIESIIAIAFSCLGILVTLFVTLIFVLYRDTPVVKSSSREL CYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSKKKICTRKPRF MSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKEVYLICNTSNLGVVAPVGYNGLLIMSCTYY AFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIA KPERNVRSAFTTSDVVRMHVGDGKLPCRSNTFLNIFRRKKPGAGNANSNGKSVSWSEPGGRQAPKGQHVW QRLSVHVKTNETACNQTAVIKPLTKSYQGSGKSLTFSDASTKTLYNVEEEDNTPSTHFSPPSSPSMVVHR RGPPVATTPPLPPHLSAEETPLFLADSVIPKGLPPPLPQQQQQPPPQPPPQQPKSLMDQLQGVVTNFGSG IPDFHAVLAGPGTPGNGLRSLYPPPPPPQHLQMLPLQLSTFREEPISPPGEDDDDDSSERFKLLQEFVYE REGNTEEDDLEEEEDLPAASKLTPEDSPALTPPSPFRDSVASGSSVPSSPVSESVLCTPPNVTYASVILR DYKQSSSTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016976 |
| ORF Size | 3597 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016976.3, NP_058672.1 |
| RefSeq Size | 6922 bp |
| RefSeq ORF | 3600 bp |
| Locus ID | 14816 |
| UniProt ID | P97772 |
| MW | 133.2 kDa |
| Gene Summary | G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum (By. similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223910 | Grm1 (untagged) - Mouse glutamate receptor, metabotropic 1 (Grm1), transcript variant 1, (10ug) |
CNY 8,352.00 |
|
| MG223498 | Grm1 (tGFP-tagged) - Mouse glutamate receptor metabotropic 1 (Grm1) transcript variant 1, (10ug) |
CNY 10,550.00 |
|
| MR223498L3 | Lenti ORF clone of Grm1 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 1 (Grm1), transcript variant 1 |
CNY 11,500.00 |
|
| MR223498L4 | Lenti ORF clone of Grm1 (mGFP-tagged) - Mouse glutamate receptor, metabotropic 1 (Grm1), transcript variant 1 |
CNY 11,500.00 |
