Pam (NM_013626) Mouse Tagged ORF Clone
CAT#: MR223751
- TrueORF®
Pam (Myc-DDK-tagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam)
ORF Plasmid: tGFP
"NM_013626" in other vectors (4)
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CNY 7,160.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PHM |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223751 representing NM_013626
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGACGCGCCCGCAGCCGCCTGCTGCTGCTGCTGGGGCTGCTCGCCTTGCAGAGCAGCTGCCTGG CCTTCAGAAGCCCACTTTCTGTCTTTAAGAGGTTTAAAGAAACTACCAGATCATTTTCCAATGAATGCCT TGGTACCACCAGACCCATCACTCCTATTGATTCTTCGGATTTTACACTGGATATTCGCATGCCTGGGGTC ACACCTAAAGAGTCTGACACGTACTTCTGCATGTCCATGCGTCTGCCTGTGGATGAGGAAGCCTTCGTGA TTGACTTCAAGCCTCGGGCCAGCATGGACACTGTCCACCATATGCTGCTGTTTGGATGCAACATGCCCTC ATCCACTGGGAGTTACTGGTTTTGTGATGAAGGAACCTGTACAGATAAAGCCAATATTCTATATGCCTGG GCAAGAAATGCTCCCCCCACCCGGCTCCCTAAAGGTGTTGGATTCAGAGTTGGAGGGGAAACTGGAAGCA AATACTTCGTCCTTCAAGTTCACTATGGGGACATCAGTGCTTTTCGAGATAATCACAAAGACTGTTCCGG TGTGTCCTTACATCTCACACGTGTGCCACAGCCTTTGATTGCGGGCATGTACCTTATGATGTCTGTCAAC ACTGTTATACCCCCAGGAGAGAAAGTAGTGAATTCTGACATTTCGTGCCATTACAAAATGTATCCAATGC ATGTGTTTGCCTACCGAGTCCATACTCACCATTTAGGTAAGGTAGTGAGTGGATACAGAGTAAGAAACGG ACAGTGGACGCTGATTGGACGCCAGAGCCCCCAGCTGCCACAGGCTTTTTACCCAGTGGAACACCCAGTA GATGTTGCTTTTGGTGATATACTGGCAGCCAGATGTGTGTTCACTGGTGAGGGGAGGACCGAAGCCACCC ACATCGGCGGCACATCTAGTGATGAAATGTGCAACTTGTACATTATGTATTACATGGAAGCCAAGCATGC AGTTTCCTTCATGACCTGTACACAGAATGTGGCTCCAGATATGTTCAGAACTATCCCAGAAGAGGCCAAT ATTCCAATTCCTGTAAAATCCGACATGGTTATGATCCATGGGCATCACAAAGAAACAGAAAACAAAGAAA AGAGTGCTTTAATACAGCAGCCAAAGCAGGGAGAGGAGGAAGCCTTCGAGCAGGGTGATTTCTATTCACT ACTTTCCAAGCTGCTAGGAGAAAGGGAAGATGTTGTTCATGTGCACAAATATAATCCTACAGAAAAGACA GAATCTGGGTCAGACCTGGTAGCTGAGATTGCAAACGTAGTCCAGAAAAAGGACCTTGGTCGGTCTGACG CCAGAGAGGGAGCGGAGCATGAGGAAGGGGGTAATGCTATCCTGGTCAGAGACAGGATCCACAAATTCCA CAGGCTAGAGTCAACCTTGAGGCCAGCTGAGAGCAGAGCTCTATCCTTCCAGCAGCCTGGTGAAGGTCCT TGGGAACCAGAACTCGCAGGAGATTTCCATGTGGAAGAAGCACTGGAGTGGCCTGGAGTGTACTTGTTAC CAGGCCAGGTTTCTGGGGTGGCCCTGGATTCTAAGAATAACCTTGTGATTTTCCACAGAGGTGACCATGT TTGGGATGGAAACTCTTTTGACAGCAAGTTCGTTTACCAACAAAGAGGTCTTGGACCAATTGAAGAAGAT ACCATCCTGGTCATTGACCCAAATAAAGCTGAAATACTCCAGTCCAGCGGCAAGAATCTGTTTTATTTAC CGCATGGTTTGAGCATAGATACAGATGGAAATTATTGGGTCACGGATGTGGCTCTCCATCAGGTGTTCAA ATTGGAACCACGTAGCAAAGAAGGCCCTCTCTTAGTTCTGGGAAGGAGCATGCAACCTGGCAGCGACCAA AATCATTTCTGCCAGCCCACTGATGTGGCGGTGGAGCCCAGTACTGGAGCTGTCTTCGTGTCAGATGGTT ACTGCAACAGTCGGATCGTGCAGTTCTCACCAAGCGGGAAGTTCATCACTCAGTGGGGAGAAGAGTCTTC TGGGAGCAGTCCTAAGCCAGGGCAGTTCAGTGTTCCTCACAGTTTGGCCCTTGTGCCTCACCTGAACCAG TTGTGTGTGGCCGATAGGGAAAATGGCCGGATCCAGTGTTTCAAGACTGACACCAAAGAATTTGTGAGAG AGATTAAGCATGCGTCATTTGGAAGGAATGTCTTTGCAATCTCATATATACCAGGTTTCCTCTTTGCAGT AAATGGGAAGCCTTACTTTGGAGACCAAGAACCCGTGCAAGGATTTGTGATGAACTTTTCCAGTGGGGAA ATTATAGATGTTTTCAAACCAGTTCGCAAGCACTTTGACATGCCTCATGATATTGTGGCTTCTGAAGATG GGACTGTGTACATTGGCGACGCACACACAAACACCGTGTGGAAGTTCACCCTCACTGAAAGCAGGCTGGA AGTGGAGCATCGATCAGTTAAAAAGGCTGGCATTGAGGTCCCGGAAATCAAAGCCGAGGCAGTTGTTGAA CCCAAAGTGAAGAACAAACCCACCTCCTCAGAATTGCAGAAGATGCAGGAGAAAAAGAAACTGATCAAAG ATCCAGGCTCGGGAGTGCCTGTGGTTCTCATTACAACCCTTCTGGTTATCCCTGTGGTTGTCCTGCTGGC CATTGCCATGTTTATTCGATGGAAAAAATCAAGGGCCTTTGGAGATCATGACCGAAAGCTCGAGTCGAGT TCTGGAAGAGTACTGGGAAGACTCCGAGGAAAGGGAAGCAGCGGCTTAAATCTAGGAAATTTCTTTGCAA GCCGGAAAGGCTACAGCAGAAAAGGGTTCGACCGAGTGAGCACAGAGGGGAGTGACCAAGAGAAGGACGA AGATGATGGAAGTGAGTCTGAAGAGGAGTACTCGGCTCCGCTGCCCACTCCTGCACCTTCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223751 representing NM_013626
Red=Cloning site Green=Tags(s) MAGRARSRLLLLLGLLALQSSCLAFRSPLSVFKRFKETTRSFSNECLGTTRPITPIDSSDFTLDIRMPGV TPKESDTYFCMSMRLPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAW ARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSLHLTRVPQPLIAGMYLMMSVN TVIPPGEKVVNSDISCHYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVEHPV DVAFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPEEAN IPIPVKSDMVMIHGHHKETENKEKSALIQQPKQGEEEAFEQGDFYSLLSKLLGEREDVVHVHKYNPTEKT ESGSDLVAEIANVVQKKDLGRSDAREGAEHEEGGNAILVRDRIHKFHRLESTLRPAESRALSFQQPGEGP WEPELAGDFHVEEALEWPGVYLLPGQVSGVALDSKNNLVIFHRGDHVWDGNSFDSKFVYQQRGLGPIEED TILVIDPNKAEILQSSGKNLFYLPHGLSIDTDGNYWVTDVALHQVFKLEPRSKEGPLLVLGRSMQPGSDQ NHFCQPTDVAVEPSTGAVFVSDGYCNSRIVQFSPSGKFITQWGEESSGSSPKPGQFSVPHSLALVPHLNQ LCVADRENGRIQCFKTDTKEFVREIKHASFGRNVFAISYIPGFLFAVNGKPYFGDQEPVQGFVMNFSSGE IIDVFKPVRKHFDMPHDIVASEDGTVYIGDAHTNTVWKFTLTESRLEVEHRSVKKAGIEVPEIKAEAVVE PKVKNKPTSSELQKMQEKKKLIKDPGSGVPVVLITTLLVIPVVVLLAIAMFIRWKKSRAFGDHDRKLESS SGRVLGRLRGKGSSGLNLGNFFASRKGYSRKGFDRVSTEGSDQEKDEDDGSESEEEYSAPLPTPAPSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013626 |
ORF Size | 2934 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013626.3, NP_038654.2 |
RefSeq Size | 4149 bp |
RefSeq ORF | 2937 bp |
Locus ID | 18484 |
UniProt ID | P97467 |
MW | 109.3 kDa |
Gene Summary | Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides (By similarity). Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (By similarity). The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (By similarity). Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222896 | Pam (untagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam), (10ug) |
CNY 12,540.00 |
|
MG223751 | Pam (tGFP-tagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam), (10ug) |
CNY 7,890.00 |
|
MR223751L3 | Lenti ORF clone of Pam (Myc-DDK-tagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam) |
CNY 9,030.00 |
|
MR223751L4 | Lenti ORF clone of Pam (mGFP-tagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam) |
CNY 9,030.00 |