Pam (NM_013626) Mouse Untagged Clone
CAT#: MC222896
Pam (untagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam), (10ug)
CNY 12,540.00
Product images
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | PHM |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC222896 representing NM_013626
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGACGCGCCCGCAGCCGCCTGCTGCTGCTGCTGGGGCTGCTCGCCTTGCAGAGCAGCTGCCTGG CCTTCAGAAGCCCACTTTCTGTCTTTAAGAGGTTTAAAGAAACTACCAGATCATTTTCCAATGAATGCCT TGGTACCACCAGACCCATCACTCCTATTGATTCTTCGGATTTTACACTGGATATTCGCATGCCTGGGGTC ACACCTAAAGAGTCTGACACGTACTTCTGCATGTCCATGCGTCTGCCTGTGGATGAGGAAGCCTTCGTGA TTGACTTCAAGCCTCGGGCCAGCATGGACACTGTCCACCATATGCTGCTGTTTGGATGCAACATGCCCTC ATCCACTGGGAGTTACTGGTTTTGTGATGAAGGAACCTGTACAGATAAAGCCAATATTCTATATGCCTGG GCAAGAAATGCTCCCCCCACCCGGCTCCCTAAAGGTGTTGGATTCAGAGTTGGAGGGGAAACTGGAAGCA AATACTTCGTCCTTCAAGTTCACTATGGGGACATCAGTGCTTTTCGAGATAATCACAAAGACTGTTCCGG TGTGTCCTTACATCTCACACGTGTGCCACAGCCTTTGATTGCGGGCATGTACCTTATGATGTCTGTCAAC ACTGTTATACCCCCAGGAGAGAAAGTAGTGAATTCTGACATTTCGTGCCATTACAAAATGTATCCAATGC ATGTGTTTGCCTACCGAGTCCATACTCACCATTTAGGTAAGGTAGTGAGTGGATACAGAGTAAGAAACGG ACAGTGGACGCTGATTGGACGCCAGAGCCCCCAGCTGCCACAGGCTTTTTACCCAGTGGAACACCCAGTA GATGTTGCTTTTGGTGATATACTGGCAGCCAGATGTGTGTTCACTGGTGAGGGGAGGACCGAAGCCACCC ACATCGGCGGCACATCTAGTGATGAAATGTGCAACTTGTACATTATGTATTACATGGAAGCCAAGCATGC AGTTTCCTTCATGACCTGTACACAGAATGTGGCTCCAGATATGTTCAGAACTATCCCAGAAGAGGCCAAT ATTCCAATTCCTGTAAAATCCGACATGGTTATGATCCATGGGCATCACAAAGAAACAGAAAACAAAGAAA AGAGTGCTTTAATACAGCAGCCAAAGCAGGGAGAGGAGGAAGCCTTCGAGCAGGGTGATTTCTATTCACT ACTTTCCAAGCTGCTAGGAGAAAGGGAAGATGTTGTTCATGTGCACAAATATAATCCTACAGAAAAGACA GAATCTGGGTCAGACCTGGTAGCTGAGATTGCAAACGTAGTCCAGAAAAAGGACCTTGGTCGGTCTGACG CCAGAGAGGGAGCGGAGCATGAGGAAGGGGGTAATGCTATCCTGGTCAGAGACAGGATCCACAAATTCCA CAGGCTAGAGTCAACCTTGAGGCCAGCTGAGAGCAGAGCTCTATCCTTCCAGCAGCCTGGTGAAGGTCCT TGGGAACCAGAACTCGCAGGAGATTTCCATGTGGAAGAAGCACTGGAGTGGCCTGGAGTGTACTTGTTAC CAGGCCAGGTTTCTGGGGTGGCCCTGGATTCTAAGAATAACCTTGTGATTTTCCACAGAGGTGACCATGT TTGGGATGGAAACTCTTTTGACAGCAAGTTCGTTTACCAACAAAGAGGTCTTGGACCAATTGAAGAAGAT ACCATCCTGGTCATTGACCCAAATAAAGCTGAAATACTCCAGTCCAGCGGCAAGAATCTGTTTTATTTAC CGCATGGTTTGAGCATAGATACAGATGGAAATTATTGGGTCACGGATGTGGCTCTCCATCAGGTGTTCAA ATTGGAACCACGTAGCAAAGAAGGCCCTCTCTTAGTTCTGGGAAGGAGCATGCAACCTGGCAGCGACCAA AATCATTTCTGCCAGCCCACTGATGTGGCGGTGGAGCCCAGTACTGGAGCTGTCTTCGTGTCAGATGGTT ACTGCAACAGTCGGATCGTGCAGTTCTCACCAAGCGGGAAGTTCATCACTCAGTGGGGAGAAGAGTCTTC TGGGAGCAGTCCTAAGCCAGGGCAGTTCAGTGTTCCTCACAGTTTGGCCCTTGTGCCTCACCTGAACCAG TTGTGTGTGGCCGATAGGGAAAATGGCCGGATCCAGTGTTTCAAGACTGACACCAAAGAATTTGTGAGAG AGATTAAGCATGCGTCATTTGGAAGGAATGTCTTTGCAATCTCATATATACCAGGTTTCCTCTTTGCAGT AAATGGGAAGCCTTACTTTGGAGACCAAGAACCCGTGCAAGGATTTGTGATGAACTTTTCCAGTGGGGAA ATTATAGATGTTTTCAAACCAGTTCGCAAGCACTTTGACATGCCTCATGATATTGTGGCTTCTGAAGATG GGACTGTGTACATTGGCGACGCACACACAAACACCGTGTGGAAGTTCACCCTCACTGAAAGCAGGCTGGA AGTGGAGCATCGATCAGTTAAAAAGGCTGGCATTGAGGTCCCGGAAATCAAAGCCGAGGCAGTTGTTGAA CCCAAAGTGAAGAACAAACCCACCTCCTCAGAATTGCAGAAGATGCAGGAGAAAAAGAAACTGATCAAAG ATCCAGGCTCGGGAGTGCCTGTGGTTCTCATTACAACCCTTCTGGTTATCCCTGTGGTTGTCCTGCTGGC CATTGCCATGTTTATTCGATGGAAAAAATCAAGGGCCTTTGGAGATCATGACCGAAAGCTCGAGTCGAGT TCTGGAAGAGTACTGGGAAGACTCCGAGGAAAGGGAAGCAGCGGCTTAAATCTAGGAAATTTCTTTGCAA GCCGGAAAGGCTACAGCAGAAAAGGGTTCGACCGAGTGAGCACAGAGGGGAGTGACCAAGAGAAGGACGA AGATGATGGAAGTGAGTCTGAAGAGGAGTACTCGGCTCCGCTGCCCACTCCTGCACCTTCCTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_013626 |
Insert Size | 2937 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013626.3, NP_038654.2 |
RefSeq Size | 4149 bp |
RefSeq ORF | 2937 bp |
Locus ID | 18484 |
UniProt ID | P97467 |
Gene Summary | Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides (By similarity). Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (By similarity). The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (By similarity). Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG223751 | Pam (tGFP-tagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam), (10ug) |
CNY 7,890.00 |
|
MR223751 | Pam (Myc-DDK-tagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam) |
CNY 7,160.00 |
|
MR223751L3 | Lenti ORF clone of Pam (Myc-DDK-tagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam) |
CNY 9,030.00 |
|
MR223751L4 | Lenti ORF clone of Pam (mGFP-tagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam) |
CNY 9,030.00 |