Mc3r (NM_008561) Mouse Tagged ORF Clone
CAT#: MR224071
- TrueORF®
Mc3r (Myc-DDK-tagged) - Mouse melanocortin 3 receptor (Mc3r)
ORF Plasmid: tGFP
"NM_008561" in other vectors (4)
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CNY 2,400.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MC3-R |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224071 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCTTCCTGCTGCCTGTCTTCTGTTTCTCCGATGCTGCCTAACCTCTCTGAGCACCCTGCAGCCC CTCCTGCCAGCAACCGGAGCGGCAGTGGGTTCTGTGAGCAGGTCTTCATCAAGCCGGAGGTCTTCCTGGC TCTGGGCATCGTCAGTCTGATGGAAAACATCCTGGTGATCCTGGCTGTGGTCAGGAATGGCAACCTGCAC TCTCCCATGTACTTCTTCCTGTGCAGCCTGGCTGCAGCCGACATGCTGGTGAGCCTGTCCAACTCCCTGG AGACCATCATGATCGCCGTGATCAACAGCGACTCCCTGACCTTGGAGGACCAGTTTATCCAGCACATGGA TAATATCTTCGACTCTATGATTTGCATCTCCCTGGTGGCCTCCATCTGCAACCTCCTGGCCATTGCCATC GACAGGTACGTCACCATCTTCTATGCCCTTCGGTACCACAGCATCATGACAGTGAGGAAAGCCCTCACCT TGATCGGGGTCATCTGGGTCTGCTGCGGCATCTGCGGCGTGATGTTCATCATCTACTCCGAGAGCAAGAT GGTCATCGTGTGTCTCATCACCATGTTCTTCGCCATGGTGCTCCTCATGGGCACCCTATATATCCACATG TTCCTCTTCGCCAGGCTCCACGTCCAGCGCATCGCAGTGCTGCCCCCTGCTGGCGTGGTGGCCCCACAGC AGCACTCCTGCATGAAGGGGGCTGTCACCATCACTATCCTGCTGGGTGTTTTCATCTTCTGCTGGGCGCC TTTCTTCCTCCACCTGGTCCTCATCATCACCTGCCCCACCAATCCCTACTGCATCTGCTACACGGCCCAT TTCAACACCTACCTGGTTCTCATCATGTGCAACTCCGTCATCGACCCCCTCATCTACGCCTTCCGCAGCC TGGAGCTGCGCAACACGTTCAAGGAGATTCTCTGCGGCTGCAACAGCATGAACTTGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224071 protein sequence
Red=Cloning site Green=Tags(s) MNSSCCLSSVSPMLPNLSEHPAAPPASNRSGSGFCEQVFIKPEVFLALGIVSLMENILVILAVVRNGNLH SPMYFFLCSLAAADMLVSLSNSLETIMIAVINSDSLTLEDQFIQHMDNIFDSMICISLVASICNLLAIAI DRYVTIFYALRYHSIMTVRKALTLIGVIWVCCGICGVMFIIYSESKMVIVCLITMFFAMVLLMGTLYIHM FLFARLHVQRIAVLPPAGVVAPQQHSCMKGAVTITILLGVFIFCWAPFFLHLVLIITCPTNPYCICYTAH FNTYLVLIMCNSVIDPLIYAFRSLELRNTFKEILCGCNSMNLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008561 |
ORF Size | 972 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008561.3, NP_032587.1 |
RefSeq Size | 2623 bp |
RefSeq ORF | 972 bp |
Locus ID | 17201 |
UniProt ID | P33033 |
MW | 35.8 kDa |
Gene Summary | This gene encodes a member of the melanocortin receptor family. Melanocortin receptors are transmembrane G-protein coupled receptors, which respond to small peptide hormones and exhibit diverse functions and tissue type localization. As part of the central nervous melanocortin system, the encoded protein is competitively bound by either melanocyte stimulating hormone or agouti-related protein to regulate energy homeostasis and adaptation to food restriction. Disruption of this gene results in an increased ratio of weight gain to food intake, increased fat mass, and decreased lean mass, without having a large effect on insulin sensitivity or glucose metabolism. [provided by RefSeq, Dec 2012] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Therapeutic senescence via GPCR activation in synovial fibroblasts facilitates resolution of arthritis
,null,
Nature Communications
,PubMed ID 32029712
[Mc3r]
|
Ligand Bias and Its Association With Pro-resolving Actions of Melanocortin Drugs
,null,
Frontiers in Pharmacology
,PubMed ID 30154720
[Mc3r]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208945 | Mc3r (untagged) - Mouse melanocortin 3 receptor (Mc3r), (10ug) |
CNY 3,990.00 |
|
MG224071 | Mc3r (tGFP-tagged) - Mouse melanocortin 3 receptor (Mc3r), (10ug) |
CNY 4,000.00 |
|
MR224071L3 | Lenti ORF clone of Mc3r (Myc-DDK-tagged) - Mouse melanocortin 3 receptor (Mc3r) |
CNY 4,750.00 |
|
MR224071L4 | Lenti ORF clone of Mc3r (mGFP-tagged) - Mouse melanocortin 3 receptor (Mc3r) |
CNY 4,750.00 |