Frem1 (NM_177863) Mouse Tagged ORF Clone
CAT#: MR224190
- TrueORF®
Frem1 (Myc-DDK-tagged) - Mouse Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 1
ORF Plasmid: tGFP
"NM_177863" in other vectors (2)
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CNY 14,464.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BC037594; crf11; D430009N09; D630008K06; eye |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224190 representing NM_177863
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCTCCAGGCTGCACTGGGCCCAAGGCTCAGTGGTTCTTGCTGCTCCAGCTGCTTCTGCTGCACC TGGACCGGGTCAGTGCCACCTTCATCAGCATCAACCGGGGCCTGAGGGTGATGAAGGGTAGTTCTGCCTT CCTGTCTGGAGATCACCTGAGGGTTGCAGTCCCTAAAGAAAAGGACGCCTGCAGACTGGAGGTGGTGATG AACGAGCCCGTAACCCAGAGGGTTGGGAAACTCTCTCCACAGGTCTTTGATTGCCATTTTCTTCCCAACG AAGTGAAGTATGTTCACAATGGATGCCCAATTCTGGATGAAGACTCTGTGAAGCTTAGACTTTACAGGTT TACTGAAACAGACACCTTCATGGAAACCTTTCTCTTGCGGGTTTATCTTGTGGAACCAGACTGTAACATC ATCCGTATGAGCAGTAATGTGTTGGAAGTGACTGAATTCTATGGCTTATCGCAAGCCATTGATAAGAACC TGCTCCAGTTTGATTATGATAGGACTGCCAGCCTGGATTGCACCATCAGACTAGACCCACTGAGGACTCA GCTGCCAGCTCACGGCAAGCTGGTCGTGGTGAATCGTAAGTCAGAGGGGCCTCGTGGAGATCAGCCACAC AGCTTCTTCTCTGAGACAGAGCTAGGGGCTGGACTGAAGTGTCCAGATGGAAGCTGTGCCCTGGAATTGA AGCAAGTGGCAAGTCTGAAAGTGAGCTGTGAGGAGTTTCTGCTGACGGGGTTTCACTACCAACACATGCA GCCCCCTTCACCCAACATTGATTACATCCCCATTCAGTTGGATCTCACCGACAGAAGGAGCAAAACCGTA TACAAGTCAGAGAGTGCGTGGTTGCCTGTCTATATCAGAGTTGGCATTCCTAACCAGGTCCCAAGGGCTG CATTTATGGCCATGTTTGTTCTGGAGGTGGACCAGTTCATACTGACCCCTCTGACAACCTCCGTTCTGGA CTGTGAGGAGGATGAGACTCCGAAACCCTTGTTGGTGTTCAACATCACTAAAGCCCCACTCCAGGGCTAT GTGACCCACCTGCTGGATCATACCAGACCCATCTCTTCATTCACCTGGAAGGATCTCAGTGACATGCAAG TTGCCTACCAGCCACCAAACAGCAGCCACCCTGAGAGGAGACATTACACGATGGAGCTGGAGGTGTACGA CTTCTTCTTTGAGAGGAGTGCCCCCATCACTGTCCACATCTCCATCAGAACAGCAGACACGAATGCACCA CGGGTGTCTTGGAATACAGGTTTGAATCTCCTAGAGGGGCAGTCTCGGGCCATCACTTGGGAACAATTTC AGATCGTTGACAATGATGACATTGGTGCTGTGCAGTTGGTCACCATTGGTGGCCTGCAGCATGGACGGCT TACAGTACGAGAGGGGAAAGGATTTCTCTTCACCGTGACTGACCTTCAAGCTGGAGTTGTCCGCTATCAT CACGATGACAGTGATACCACCAAAGACTTTGTGGCTTTCAGGATATTCGATGGCCACCACAGCAGCCACC ACAAGTTTCCTATCAACATCTTGCCTAAAGATGACAGCCCCCCATTCCTCATCACCAATGTCGTGATTGA ACTAGAAGAGGGGAAGACCATCTTGATCCAAGGGTCTATGTTGAGAGCTTCAGATATGGACTCCAGTGAT GACTACATCTTCTTTAATATCACAAAGTTTCCACAGGCTGGGGAGATCATGAAGAAGCCAGGGCCAAGAC TGATAGGGTATCCTGTCCCTGGCTTCCTGCAGAGAGACCTGTTCAGTGGAATCATTTACTATCGTCACTT TGGTGGAGAAATCTTTGAAGATTCCTTTGAATTCGTACTGTGGGACAGCCATGAGCCTCCAAATCTCTCA GTGCCACAGGTGGTGACAATTCATATCACTCCAGTAGATGACCAGCTTCCTAAAGAAGCTCCTGGCATTT CCCGGCATTTGGTTGTCAAGGAAACAGAGGTGGCCTACATCACTAAAAAGCACCTACATTTCCTAGACAT GGAATCCCGTGATGGGGAACTCATCTACACAGTAACACGCCCTCCATGTTTCTCCTTCAGCCACAGACAC TTGGATGCTGGGAAGCTATTCATGGTGGACAGCATACCAAAGCTAACCAAAAATCCGACAGCCCCAGGGC TAAGTTCATTTACTCAGGGCTTGATTCTGATCTCTGCAAATCAGCACACGTGTTCATCTCAATTTGCTTC ACAGCATGCCGTGAACCATATGAAAGTGGCCTACATGCCACCCATGCAAGATATAGGCCCCTCCCCTCGA CATGTGCAGTTCACAGTGTCTGTCAGTAACCAGCATGGAGGTGCTTTGCATGGAATCTGCTTCAACATCA CAGTTCTCCCAGTGGACAACCAGGTTCCCGAGGTATTCACCAACACCCTGAGAGTGGTCGAGGGCGGTCA GTGCACCATCAGCACAGAGCACATTTTGGTTTCTGATGTGGACACCCCGCTAGACAGCATCAGCCTCTCT CTTAAGGAGAGGCCTCTGCATGGCGGGGTAGAGCTGGACGGATTTCCTCTAAACCCAAGAGGCACATTTT CTTGGCGAGACCTCAATACCTTAAAAGTTTGGTATCAACATGATGGATCTGAGGTTCTTCAGGATGAGAT ATTTTTGGAGGTCACAGATGGCACAAATTCAGCAGCATTTGTTCTCCACATTGAGGTGTTCCCTGTGAAT GATGAGCCACCCATTCTGAAGGCTGACCTCATCCCCATGATGCACTGCTCTGAGGGTGGAGAGGTGACCA TCACCCCTGAATACATTTCCGCTACTGATGCAGACAGCGATGACTTAGAACTGCTGTTTCTGATTGCCCG AGAGCCTCAGCATGGAGTGGTGAGAAAGGCTGGACTTCACGTGGACAGGTTCTCTCAGGGAGATGTCATC TCAGGGGCTGTGACATACAAACATACAGGTGGCGAGATTGGCCTTGAACCTTGTTCTGACACTGTTGTAT TGGTGGTTTCGGATGGTGAAGCTGACCCTTTGATGAACGGCTGTTGCTACGATGGACCTGATTCATCTGT TCCTCTTCATAAGTCCTTCCCCACATACCAGCTCAACATCACAGTACATCCAGTCGACAACCAGCCACCT TCAATTATAATAGGTAGGATGCTCACAGTGGATGAAGGCTTCTCAGCAGCCCTTACTACTCATCACTTGA CTGTTGTTGATTGGGACACTGCCCCGGATGACTTAAAATTCATGTTGGCTTCTCAGCCCCAGTTTGGCTA CTTAGAAAACGCACTGCCTTCTGCAGGCTTTGAGAAAAGTAACATCGGCATAAGGATAGCCTCATTCCAG TGGACAGACATGAAGGCTTCACACATTAACTATGTGCAGTCCAGGCACCTGAGAGTGGAACCAACTGCTG ACCAGTTCACAGTATATGCCACAGACGGGAAGCATCGCTCCTTGGAGACAACATTTCACGTTATCATCAA CCCCACAAACGATGAAGCTCCTGACCTTGCAGTACAGAACATCACTGTGTATGAGGGGCACATGGTAGAA CTGGATTCTTCCATCATCAGTGCTACTGACAGGGATATTCCCAAGGACCCCTTGCTCTTCAGCATCGCTC TCAAGCCCCAGCACGGCCTGCTCGTCGATGCTGCCATCAGCAAGGACTCCCATCAGATCAAGCAGCTGCA ACACGAAATCCACAGCTTCTCCGTGGACCTTCTCAAGAATGGAATGAAGCTGGTCTATGCCCACGACGAC TCAGAGAGCTCTGCTGATAACTTCGTGATCCAGTTGTCAGACGGGAAACATAAAATCCTTAAAACCATCT CAGTAAACATTACCCCAGTGAATGACGAGACACCAACGCTAAGCAAGAAGGCTGAAATCTCAATGGCTGT GGGCGATACTCGCGTCCTGTCTAGTGCTGTTCTTTCAGCCACAGATAAAGACTCGCCCAGAGAGAAGATT CACTATGTATTTGAAAGACTTCCCCAAAATGGACAACTTCAACTTAAGATAGGGAGGGACTGGGTCCCTC TCTCCACTGGCATGCAGTGCACCCAGGAGGATGTGGACCTGAACTTGCTAAGATACACGCATGCTGGTAA AACGGACTCCCAGGATGGAGACAGCTTCACCTTCTACCTCTGGGATGAGGACAACAGGTCACCTGCATTT GACTGTCACATCATCATTGAAGACATGGGCAAAGGTGATATTGTCATTCATGCAAAACCACTCGTGGTAG TCAAAGGTGACAGAGGTCTCTTGACGACTGCCACCCTTCTGGCTGTGGATGGAGCAGACAAGCCTGAGGA ACTGCTCTACCTCATCACCTCCCCACCACGGCATGGCCAAGTGGAGTACGTCCACTCTCCTGGAGTCCCC ATTGCCAGCTTCAGCCAAATGGACATAGCAGGACAGACAGTGTGCTACATACACAAGAGCAGGACAGCTG TCCCCACTGACAGCTTCAGATTTACCATCAGCAATGGACTGCAGACCCAGCGTGGGGTGTTTGAAATCAC ACTGCAGACTGTGGACAGCGCCTTGCCTGTGCTGACCAAGAACAAAAGGCTGAGGCTAGCCGAAGGGGCC ATGGGCCTCCTGTCCGCTGATCACCTTCAGCTGACTGACCCCGACACACCTCCAGAGAACCTGACCTTCT TTCTGGCTCAGCTCCCACGCCACGGGTACCTCTTCCTGAGAGGGAAAGCACTCCAACACAACTTCACCCA GCGAGATGTGGACAGCGGGGGCGTGGCCTACCAGCACTCAGGAGGCGGCGCCCGGGAGGACTATTTTACT TTTCTAGCCACAGACAGGAAGAACCAAGGCTTTGTTGTGGATGGGAAAGTTCAGAAAGAGCCTGTTCGCT TCACAATCCAGGTGGATCAGCTGGACAAGGCAGCGCCCCGTATCACACACTTGCACTCCCCTACTCAAGT GGGGCTCTTGAAAAATGGCTGCTATGGGATTTACATCACTTCCCGTGTGCTGAAGGCATCAGACCCTGAC ACAGAGGATGACCAGATCATCTTTAAGATTTTACGAGGCCCATTGTACGGACGTCTGGAGAACACAACAA CAGGTGAATTCATCCACGAGCGATTTAGCCAGAAGGACTTAAGCCATAAGACCATCCTTTACATCATAAA CCCTTCTTTGCAAGTGACTTCCGATATCCTGGAATTTCAGGCCATGGACCCCACAGGGAACACTGCCACT CCCCAGAGCTTGGACTTGAGATGGTCGTATATTGAATGGGCCCAGACTGCATATGAGGTTTGTGAGAATG TGGGCTTATTGCCCTTGGAAGTTACCAGAAGGGGATATCCCATGGACTCAGCCTTTGTGGGTGTAGAGGT TAACCAAGTGTCAGCTACAGTTGGAAAAGATTTTACTGTCACTCCATCCAAACTGCTTCAATTTGACCCA GGAATGTCAACTAAGATGTGGAATATAGCAATTACCTATGACGGATTAGAGGAAGATGATGAGGTCTTTG AAGTAATTCTGAACTCCCCTGTGAATGCGGTTCTTGGCACGCAGACAAAAGCGGCGGTGAAAATTTTGGA CTCAAAAGGAGGACGGTGCCATCCTTCAAATTCCTTCAACCAAAGCAAGCACAGCACGTGGGGGAAGGGC CCTTGGCATCCGCTGCCCTCAGGCTCATCCTCGCTCACCACTTCAGGTTCTCCTCTTCTGGAAAGGCCTC CTCCATCTTTCACCAGTGGAGATGCTCTTCAGGGTTTTGGTCTCACCGACCTCACTCAAAGGAAGACAAT GACCCAGGGGAATGGCAAATCAGTTCTCCCATCCTCTGTTTGTAGAAATGGGACAGACACCATCTACAAT TACCATGGTATAGTTTCCTTGAAACTAGAGGGCGACAGATTCTCAGCTCACAAACGGAAGGCCAAAATAT CCATTGTTAGTCAGCCACAGAGGACAATCAAGGTGGCAGAGCTGCCTCTAGCTGATAAGGTGGAATCCAC AACTGATTTGCACTTTCTCAGACAGGGTCTGCGGCCCTTGTTTCCAAAGAATTGTTCTGTGGACTTAAAG GGACTCTTTCATTTTGAAGAAAGCACCCACAGATTGTATCAATGTGATGGGATCTCCTGGAAAGCCTGGA GCCCCCAAACCAAGGGGCTGGAGGACAGATCCTGCCCAGGTGGATGGCTCCTTCATTCTGGCTACTGTCA CATCTTGGTCACAAGACAGAAAGGCACCTGGACCACAGCTACCCGAGCTTGCAGAGAACAGCACCAGGGC GACCTTGTGACCGTACTCTCCAGAAGGCATATGCAGTGGCTATGGGCGATGAGTGGAAGAAAACCCTTCT GGATAGGCTTGAAGAACCAACCGAGGACTGGCCACTGGGAGTGGATCGGAGGTGAACCTGTTGCTTTTAC CAACTGGAGGAGAGGGGCCCCTCTGCACCCAAAACCTGGAAAGAACTGTGCTTTGGTTCAGAAAAGAGGA CAATGGCAGACAAAGAACTGTAGCAAAGGCAAGGCTCACAATTTTGTGTGCTCAAGGAAACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224190 representing NM_177863
Red=Cloning site Green=Tags(s) MHSPGCTGPKAQWFLLLQLLLLHLDRVSATFISINRGLRVMKGSSAFLSGDHLRVAVPKEKDACRLEVVM NEPVTQRVGKLSPQVFDCHFLPNEVKYVHNGCPILDEDSVKLRLYRFTETDTFMETFLLRVYLVEPDCNI IRMSSNVLEVTEFYGLSQAIDKNLLQFDYDRTASLDCTIRLDPLRTQLPAHGKLVVVNRKSEGPRGDQPH SFFSETELGAGLKCPDGSCALELKQVASLKVSCEEFLLTGFHYQHMQPPSPNIDYIPIQLDLTDRRSKTV YKSESAWLPVYIRVGIPNQVPRAAFMAMFVLEVDQFILTPLTTSVLDCEEDETPKPLLVFNITKAPLQGY VTHLLDHTRPISSFTWKDLSDMQVAYQPPNSSHPERRHYTMELEVYDFFFERSAPITVHISIRTADTNAP RVSWNTGLNLLEGQSRAITWEQFQIVDNDDIGAVQLVTIGGLQHGRLTVREGKGFLFTVTDLQAGVVRYH HDDSDTTKDFVAFRIFDGHHSSHHKFPINILPKDDSPPFLITNVVIELEEGKTILIQGSMLRASDMDSSD DYIFFNITKFPQAGEIMKKPGPRLIGYPVPGFLQRDLFSGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLS VPQVVTIHITPVDDQLPKEAPGISRHLVVKETEVAYITKKHLHFLDMESRDGELIYTVTRPPCFSFSHRH LDAGKLFMVDSIPKLTKNPTAPGLSSFTQGLILISANQHTCSSQFASQHAVNHMKVAYMPPMQDIGPSPR HVQFTVSVSNQHGGALHGICFNITVLPVDNQVPEVFTNTLRVVEGGQCTISTEHILVSDVDTPLDSISLS LKERPLHGGVELDGFPLNPRGTFSWRDLNTLKVWYQHDGSEVLQDEIFLEVTDGTNSAAFVLHIEVFPVN DEPPILKADLIPMMHCSEGGEVTITPEYISATDADSDDLELLFLIAREPQHGVVRKAGLHVDRFSQGDVI SGAVTYKHTGGEIGLEPCSDTVVLVVSDGEADPLMNGCCYDGPDSSVPLHKSFPTYQLNITVHPVDNQPP SIIIGRMLTVDEGFSAALTTHHLTVVDWDTAPDDLKFMLASQPQFGYLENALPSAGFEKSNIGIRIASFQ WTDMKASHINYVQSRHLRVEPTADQFTVYATDGKHRSLETTFHVIINPTNDEAPDLAVQNITVYEGHMVE LDSSIISATDRDIPKDPLLFSIALKPQHGLLVDAAISKDSHQIKQLQHEIHSFSVDLLKNGMKLVYAHDD SESSADNFVIQLSDGKHKILKTISVNITPVNDETPTLSKKAEISMAVGDTRVLSSAVLSATDKDSPREKI HYVFERLPQNGQLQLKIGRDWVPLSTGMQCTQEDVDLNLLRYTHAGKTDSQDGDSFTFYLWDEDNRSPAF DCHIIIEDMGKGDIVIHAKPLVVVKGDRGLLTTATLLAVDGADKPEELLYLITSPPRHGQVEYVHSPGVP IASFSQMDIAGQTVCYIHKSRTAVPTDSFRFTISNGLQTQRGVFEITLQTVDSALPVLTKNKRLRLAEGA MGLLSADHLQLTDPDTPPENLTFFLAQLPRHGYLFLRGKALQHNFTQRDVDSGGVAYQHSGGGAREDYFT FLATDRKNQGFVVDGKVQKEPVRFTIQVDQLDKAAPRITHLHSPTQVGLLKNGCYGIYITSRVLKASDPD TEDDQIIFKILRGPLYGRLENTTTGEFIHERFSQKDLSHKTILYIINPSLQVTSDILEFQAMDPTGNTAT PQSLDLRWSYIEWAQTAYEVCENVGLLPLEVTRRGYPMDSAFVGVEVNQVSATVGKDFTVTPSKLLQFDP GMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTQTKAAVKILDSKGGRCHPSNSFNQSKHSTWGKG PWHPLPSGSSSLTTSGSPLLERPPPSFTSGDALQGFGLTDLTQRKTMTQGNGKSVLPSSVCRNGTDTIYN YHGIVSLKLEGDRFSAHKRKAKISIVSQPQRTIKVAELPLADKVESTTDLHFLRQGLRPLFPKNCSVDLK GLFHFEESTHRLYQCDGISWKAWSPQTKGLEDRSCPGGWLLHSGYCHILVTRQKGTWTTATRACREQHQG DLVTVLSRRHMQWLWAMSGRKPFWIGLKNQPRTGHWEWIGGEPVAFTNWRRGAPLHPKPGKNCALVQKRG QWQTKNCSKGKAHNFVCSRKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_177863 |
ORF Size | 6573 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177863.5 |
RefSeq Size | 9307 bp |
RefSeq ORF | 6576 bp |
Locus ID | 329872 |
UniProt ID | Q684R7 |
MW | 244.5 kDa |
Gene Summary | Extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |