United States
Germany
Japan
United Kingdom
China
Myo1b (NM_001161817) Mouse Tagged ORF Clone
CAT＃: MR224289
- TrueORF®
Myo1b (Myc-DDK-tagged) - Mouse myosin IB (Myo1b), transcript variant 1
ORF Plasmid: tGFP
"NM_001161817" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 7,912.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA406997; MIH-L; MMIa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224289 representing NM_001161817
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAAATGGAGGTGAAATCCTCACTTCTGGACAATATGATCGGTGTTGGAGATATGGTTCTTTTAG AGCCGCTCAATGAGGAGACGTTCATCGACAACCTGAAGAAGCGCTTCGACCACAATGAGATATATACATA CATTGGGAGCGTGGTTATATCTGTTAACCCCTACCGGTCCTTGCCCATATATTCGCCGGAGAAAGTGGAA GATTACAGAAATAGAAACTTCTATGAACTGAGCCCTCACATCTTTGCTTTGTCGGATGAAGCATACAGAT CCCTCCGTGATCAAGATAAAGATCAATGCATTCTCATTACTGGGGAGAGTGGAGCAGGAAAAACAGAGGC CAGCAAGCTCGTCATGTCCTATGTCGCTGCCGTGTGCGGAAAAGGAGCAGAAGTCAATCAGGTCAAGGAA CAGCTTCTGCAGTCCAACCCTGTCCTGGAAGCTTTTGGAAATGCCAAGACTGTGAGGAATGACAACTCAT CTAGATTCGGCAAATACATGGATATTGAATTTGACTTTAAAGGCGATCCGCTGGGAGGAGTGATAAGTAA CTATCTTTTAGAGAAATCTCGTGTTGTTAAGCAGCCAAGAGGTGAAAGAAACTTCCATGTGTTCTATCAG CTGCTCTCAGGCGCCTCTGAGGAGCTCCTCTACAAGCTTAAGCTGGAGCGGGATTTCAGCAGATACAACT ATCTGAGTCTGGACTCTGCTAAAGTTAATGGTGTGGATGATGCTGCCAATTTCAGAACTGTTAGGAATGC GATGCAGATCGTGGGCTTTCTGGATCATGAGGCCGAGGCTGTCCTGGAGGTAGTGGCAGCCGTTTTGAAA CTGGGGAACATCGAGTTCAAGCCTGAATCACGAGTGAATGGTTTGGATGAAAGCAAAATCAAAGATAAAA ATGAGTTAAAAGAAATTTGCGAGTTGACCAGCATCGATCAGGTGGTTTTAGAAAGAGCGTTCAGTTTCCG AACAGTTGAAGCGAAGCAGGAGAAAGTTTCAACCACGCTGAATGTGGCTCAGGCTTATTATGCCCGTGAT GCCCTGGCTAAAAACCTCTACAGCCGCTTGTTTTCATGGTTGGTAAATCGTATCAACGAAAGCATTAAGG CACAAACAAAAGTGAGAAAGAAGGTCATGGGTGTTCTGGACATTTATGGCTTTGAAATATTTGAGGATAA CAGCTTCGAGCAGTTTATTATTAACTATTGTAATGAAAAGCTTCAACAAATCTTCATCGAACTTACCCTC AAAGAAGAGCAAGAGGAATATATTCGGGAGGACATAGAATGGACTCACATTGACTACTTCAACAATGCTA TCATTTGTGACCTAATAGAAAATAACACAAATGGAATCTTGGCCATGCTGGATGAAGAGTGCCTGAGACC CGGCACTGTTACAGATGAGACCTTCCTGGAAAAGCTGAACCAAGTCTGTGCCACCCACCAGCACTTTGAG AGCAGGATGAGCAAGTGTTCCCGCTTTCTCAACGACACAACCCTGCCCCACAGCTGCTTCAGAATCCAGC ATTATGCTGGCAAGGTGCTCTACCAGGTGGAAGGATTTGTTGACAAGAACAATGACCTTCTGTACCGGGA CCTGTCTCAAGCCATGTGGAAGGCCGGCCACTCCCTCATCAAGTCTCTGTTTCCTGAAGGAAATCCTGCA AAGGTCAACCTGAAGAGACCTCCCACCGCAGGCTCCCAGTTCAAGGCGTCTGTGGCCACGCTGATGAGAA ACCTGCAGACCAAGAACCCGAACTACATCAGGTGCATCAAGCCAAATGATAAAAAGGCAGCTCACATTTT CAATGAGAGTCTCGTATGCCATCAGATCAGGTACCTGGGTCTTTTGGAGAACGTTCGAGTGAGACGGGCA GGCTATGCCTTCAGGCAGGCCTATGAACCTTGCCTGGAAAGATACAAGATGCTTTGCAAACAAACGTGGC CTCACTGGAAGGGACCAGCAAGGTCTGGCGTGGAGGTTCTGTTTAATGAGCTAGAGATTCCTGTGGAAGA GCACTCCTTCGGGAGATCCAAGATATTCATCCGAAACCCACGGACATTATTCCAACTAGAAGACCTAAGG AAGCAGCGGCTGGAGGACCTGGCCACTCTCATTCAGAAGATTTATCGAGGATGGAAATGCCGTACACATT TTCTGCTCATGAAAAGAAGCCAAGTTGTGATTGCTGCCTGGTACAGGAGATATGCGCAACAAAAGCGGTA CCAGCAGATAAAGAGTTCAGCCCTGGTGATTCAGTCGTACATCCGGGGCTGGAAGGCTCGAAAAATCCTG CGGGAGCTGAAGCATCAGAAGCGCTGTAAGGAGGCAGCCACCACCATCGCGGCGTACTGGCATGGCACCC AGGCGCGAAGGGAGCTGAGACGGCTGAAGGACGAGGCTAGGAATAAGCACGCTATTGCAGTTATTTGGGC TTACTGGCTTGGATCGAAGGCACGGAGGGAGTTGAAACGCTTGAAGGAGGAGGCTAGGCGTAAGCATGCA GTTGCTGTCATTTGGGCTTACTGGCTTGGACTGAAGGTACGCAGGGAATACAGGAAATTCTTTAGAGCAA ATGCTGGAAAGAAAATCTATGAGTTTACACTTCAAAGAATTGTGCAAAAATACTTGTTGGAAATGAAAAA TAAAATGCCTTCCTTGTCACCGATAGATAAAAATTGGCCATCAAGACCTTACCTGTTCTTGGATTCGACT CACAAGGAGCTAAAAAGGATCTTCCACTTGTGGAGGTGTAAAAAATACAGGGATCAATTCACAGACCAGC AGAAACTTATTTATGAAGAGAAGCTCGAGGCCAGCGAACTCTTCAAAGACAAGAAGGCTTTATATCCTTC TAGTGTTGGGCAACCATTCCAAGGAGCTTACCTGGAAATCAACAAGAACCCAAAATATAAGAAACTCAAA GATGCCATTGAAGAGAAGATCATTATTGCTGAGGTTGTGAACAAAATTAATCGGGCTAATGGGAAGAGTA CATCTCGGATTTTCCTCTTAACAAACAATAACCTTCTCCTTGCTGACCAAAAGTCTGGGCAGATCAAGTC CGAGGTCCCCCTGGTAGATGTGACCAAGGTTTCCATGAGTTCCCAGAACGATGGTTTCTTTGCAGTGCAC CTCAAGGAGGGTTCAGAAGCGGCTAGTAAAGGGGACTTTCTCTTCAGCAGTGACCACCTGATTGAAATGG CAACCAAGCTGTATCGCACGACTCTCAGCCAAACTAAACAGAAGCTCAACATCGAGATTTCCGATGAGTT CCTGGTACAGTTCAGACAGGACAAAGTATGTGTGAAGTTTATTCAAGGCAACCAGAAAAATGGGAGCGTG CCAACCTGCAAACGAAAGAACAACAGACTCCTTGAAGTCGCTGTCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224289 representing NM_001161817
Red=Cloning site Green=Tags(s) MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVE DYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKE QLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQ LLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLK LGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARD ALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFEDNSFEQFIINYCNEKLQQIFIELTL KEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFE SRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPA KVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRA GYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLR KQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKIL RELKHQKRCKEAATTIAAYWHGTQARRELRRLKDEARNKHAIAVIWAYWLGSKARRELKRLKEEARRKHA VAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDST HKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLK DAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVH LKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSV PTCKRKNNRLLEVAVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001161817 |
| ORF Size | 3408 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001161817.2, NP_001155289.1 |
| RefSeq Size | 5053 bp |
| RefSeq ORF | 3411 bp |
| Locus ID | 17912 |
| MW | 132.1 kDa |
| Gene Summary | Motor protein that may participate in process critical to neuronal development and function such as cell migration, neurite outgrowth and vesicular transport.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224289 | Myo1b (tGFP-tagged) - Mouse myosin IB (Myo1b) transcript variant 1, (10ug) |
CNY 10,830.00 |
|
| MR224289L3 | Lenti ORF clone of Myo1b (Myc-DDK-tagged) - Mouse myosin IB (Myo1b), transcript variant 1 |
CNY 11,780.00 |
|
| MR224289L4 | Lenti ORF clone of Myo1b (mGFP-tagged) - Mouse myosin IB (Myo1b), transcript variant 1 |
CNY 11,780.00 |
