Pde4b (NM_019840) Mouse Tagged ORF Clone
CAT#: MR224769
- TrueORF®
Pde4b (Myc-DDK-tagged) - Mouse phosphodiesterase 4B, cAMP specific (Pde4b), transcript variant 1
ORF Plasmid: tGFP
"NM_019840" in other vectors (4)
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CNY 7,220.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Dpde4; dunce; R74983 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224769 representing NM_019840
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCAAAAAATTCTCCAAAAGAATTTACTGCTTCGGAATCTGAGGTTTGCATAAAGACTTTCAAGG AGCAGATGCGCTTGGAACTTGAGCTTCCAAAGCTACCAGGAAACAGACCTACATCTCCCAAAATTTCTCC ACGCAGTTCACCAAGGAATTCACCATGCTTTTTCAGAAAGTTGCTGGTGAATAAAAGCATCCGACAGCGG CGTCGCTTCACGGTGGCTCATACATGCTTTGATGTGGAAAATGGCCCTTCTCCAGGTCGGAGCCCACTGG ACCCTCAAGCCGGCTCTTCGTCGGGACTGGTACTTCATGCCGCCTTTCCTGGGCACAGCCAGCGCAGGGA GTCGTTCCTCTACAGATCTGACAGCGACTATGACTTGTCACCAAAAGCGATGTCCAGGAACTCATCACTT CCCAGTGAGCAACACGGCGATGACCTGATTGTCACTCCTTTTGCCCAGGTTCTTGCCAGCTTGCGAAGTG TAAGAAACAACTTCACCCTGCTGACGAACCTTCATGGAGCGCCGAACAAGAGGTCACCAGCGGCTAGTCA GGCTCCAGTCTCCAGAGTCAGCCTGCAAGAAGAATCATATCAGAAACTAGCAATGGAGACGCTGGAGGAA CTAGACTGGTGCCTAGACCAGCTAGAGACCATCCAGACCTACCGCTCTGTCAGCGAGATGGCTTCAAACA AGTTCAAAAGGATGCTGAACCGGGAGCTGACACACCTCTCAGAGATGAGCAGATCAGGGAACCAGGTGTC TGAGTACATTTCAAACACGTTCTTAGACAAGCAGAACGATGTGGAAATCCCATCTCCCACGCAGAAGGAC AGGGAGAAGAAGAAGAAGCAGCAGCTCATGACCCAGATAAGTGGAGTGAAGAAACTGATGCACAGCTCAA GCCTGAACAACACAAGCATCTCACGCTTCGGAGTCAACACGGAAAATGAGGATCATCTAGCCAAGGAGCT GGAAGACCTGAACAAATGGGGCCTTAACATCTTCAATGTGGCTGGGTACTCACATAATCGGCCCCTTACG TGCATCATGTATGCAATATTCCAGGAAAGAGACCTTCTGAAGACGTTTAAAATCTCATCTGACACCTTTG TAACCTACATGATGACTTTAGAAGACCATTACCATTCTGATGTGGCATATCACAACAGCCTGCATGCTGC TGACGTGGCCCAGTCAACTCACGTTCTCCTTTCTACGCCGGCACTGGATGCTGTCTTCACAGACCTGGAA ATCCTGGCTGCCATTTTTGCAGCTGCCATCCATGATGTCGATCATCCTGGAGTCTCCAATCAGTTTCTCA TCAATACAAATTCTGAACTTGCTTTGATGTATAATGATGAATCTGTTCTGGAAAACCATCACCTTGCTGT GGGATTCAAATTGCTACAAGAGGAACACTGCGACATCTTTCAGAATCTTACCAAGAAGCAACGCCAGACA CTCAGGAAAATGGTGATTGACATGGTGTTGGCAACTGATATGTCCAAACACATGAGCCTCCTGGCAGACC TTAAAACAATGGTAGAAACCAAGAAGGTGACAAGCTCCGGTGTTCTCCTCCTGGACAACTATACTGACCG GATACAGGTTCTTCGCAACATGGTACACTGTGCAGACCTGAGCAACCCCACCAAGTCCTTGGAATTGTAT CGGCAATGGACCGATCGTATCATGGAGGAGTTTTTCCAGCAGGGAGACAAAGAACGGGAGAGGGGAATGG AGATTAGCCCAATGTGTGATAAGCACACAGCTTCTGTGGAAAAATCCCAGGTTGGTTTCATTGACTACAT TGTCCATCCACTGTGGGAGACCTGGGCAGACCTGGTTCAACCGGATGCTCAAGATATTCTGGATACACTA GAAGATAACAGGAACTGGTACCAGAGTATGATACCCCAGAGCCCTTCCCCGCCACTGGATGAGAGGAGCA GGGACTGCCAAGGCCTGATGGAGAAGTTTCAGTTTGAACTGACCCTTGAGGAAGAGGATTCTGAGGGACC GGAAAAGGAGGGAGAAGGCCACAGCTATTTCAGCAGCACAAAGACGCTTTGTGTGATTGATCCAGAGAAC AGGGATTCTCTGGAAGAGACTGACATAGACATTGCAACAGAAGACAAGTCTCCGATCGACACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224769 representing NM_019840
Red=Cloning site Green=Tags(s) MTAKNSPKEFTASESEVCIKTFKEQMRLELELPKLPGNRPTSPKISPRSSPRNSPCFFRKLLVNKSIRQR RRFTVAHTCFDVENGPSPGRSPLDPQAGSSSGLVLHAAFPGHSQRRESFLYRSDSDYDLSPKAMSRNSSL PSEQHGDDLIVTPFAQVLASLRSVRNNFTLLTNLHGAPNKRSPAASQAPVSRVSLQEESYQKLAMETLEE LDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQNDVEIPSPTQKD REKKKKQQLMTQISGVKKLMHSSSLNNTSISRFGVNTENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLT CIMYAIFQERDLLKTFKISSDTFVTYMMTLEDHYHSDVAYHNSLHAADVAQSTHVLLSTPALDAVFTDLE ILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQT LRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELY RQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTL EDNRNWYQSMIPQSPSPPLDERSRDCQGLMEKFQFELTLEEEDSEGPEKEGEGHSYFSSTKTLCVIDPEN RDSLEETDIDIATEDKSPIDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019840 |
ORF Size | 2163 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019840.2, NP_062814.2 |
RefSeq Size | 4371 bp |
RefSeq ORF | 2166 bp |
Locus ID | 18578 |
MW | 82.5 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221083 | Pde4b (untagged) - Mouse phosphodiesterase 4B, cAMP specific (Pde4b), transcript variant 1, (10ug) |
CNY 9,310.00 |
|
MG224769 | Pde4b (tGFP-tagged) - Mouse phosphodiesterase 4B cAMP specific (Pde4b) transcript variant 1, (10ug) |
CNY 7,980.00 |
|
MR224769L3 | Lenti ORF clone of Pde4b (Myc-DDK-tagged) - Mouse phosphodiesterase 4B, cAMP specific (Pde4b), transcript variant 1 |
CNY 9,120.00 |
|
MR224769L4 | Lenti ORF clone of Pde4b (mGFP-tagged) - Mouse phosphodiesterase 4B, cAMP specific (Pde4b), transcript variant 1 |
CNY 9,120.00 |