Unc93b1 (NM_001161428) Mouse Tagged ORF Clone

CAT#: MR224858

  • TrueORF®

Unc93b1 (Myc-DDK-tagged) - Mouse unc-93 homolog B1 (C. elegans) (Unc93b1), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001161428" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,180.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Unc-93B1; Unc93b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR224858 representing NM_001161428
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAAGTCCCAACCAGCTGCTGGTGCCCAGAACTGCAAGCCCTTGATCTGGATCTGGTCATGGAGG
TGGAGCCTCCGCTCTACCCTGTGGCCGGGGCCGCGGGTCCTCAAGGGGATGAAGACCGGCACGGAGTTCC
TGATGGGCCAGAGGCTCCCTTGGACGAACTCGTGGGTGCGTACCCCAACTACAATGAGGAGGAGGAAGAG
CGCCGCTACTACCGCCGCAAGCGCCTCGGAGTGGTCAAGAACGTGCTGGCGGCCAGCACGGGTGTCACCC
TTACTTACGGCGTCTACCTGGGCCTCCTGCAGATGCAACTGATCCTGCACTATGATGAGACCTACAGAGA
GGTGAAGTATGGCAACATGGGGCTGCCGGACATCGATAGCAAGATGCTGATGGGTATCAACGTGACGCCT
ATCGCTGCCCTGCTCTACACACCTGTGCTCATCAGGTTTTTTGGTACCAAGTGGATGATGTTCTTGGCTG
TGGGCATCTATGCCCTCTTTGTCTCTACCAACTACTGGGAACGCTACTACACGCTGGTGCCCTCTGCTGT
GGCTCTGGGCATGGCCATTGTGCCTCTGTGGGCCTCCATGGGCAACTATATCACCAGGATGTCCCAGAAG
TACTATGAATACTCCCACTACAAGGAGCAAGATGAGCAGGGACCTCAGCAGCGCCCACCACGAGGTTCCC
ACGCACCCTATCTCCTGGTTTTCCAGGCCATCTTCTATAGCTTCTTCCACTTGAGCTTCGCGTGTGCCCA
GCTGCCCATGATTTACTTCCTCAACAACTACCTGTATGACCTGAACCACACACTGATCAACGTGCAGAGC
TGCGGTACTAAGAGCCAAGGCATTCTGAATGGCTTCAACAAGACGGTCCTTCGGACGCTGCCGCGCAGCA
AAAACCTTATTGTTGTAGAGAGCGTGCTCATGGCGGTGGCCTTCTTGGCCATGCTGATGGTGCTGGGCCT
GTGTGGAGCCGCTTACCGGCCCACGGAAGAGATCGACCTGCGCAGCGTGGGCTGGGGCAACATCTTCCAG
CTGCCCTTCAAACACGTGCGTGACTTTCGCTTACGCCATCTGGTGCCCTTCTTTATCTACAGTGGCTTTG
AGGTGCTCTTTGCCTGCACTGGTTTTGCCCTGCACTCCTGGGCATCCTATATGAAGACAAAGAGAGGCAG
GACTTCATCTTCACCATCTATCACTGGTGGCAGGCCGTGGCCATCTTTGTTGTGTACCTGGGCTCCAGCT
TGCCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR224858 representing NM_001161428
Red=Cloning site Green=Tags(s)

MKEVPTSCWCPELQALDLDLVMEVEPPLYPVAGAAGPQGDEDRHGVPDGPEAPLDELVGAYPNYNEEEEE
RRYYRRKRLGVVKNVLAASTGVTLTYGVYLGLLQMQLILHYDETYREVKYGNMGLPDIDSKMLMGINVTP
IAALLYTPVLIRFFGTKWMMFLAVGIYALFVSTNYWERYYTLVPSAVALGMAIVPLWASMGNYITRMSQK
YYEYSHYKEQDEQGPQQRPPRGSHAPYLLVFQAIFYSFFHLSFACAQLPMIYFLNNYLYDLNHTLINVQS
CGTKSQGILNGFNKTVLRTLPRSKNLIVVESVLMAVAFLAMLMVLGLCGAAYRPTEEIDLRSVGWGNIFQ
LPFKHVRDFRLRHLVPFFIYSGFEVLFACTGFALHSWASYMKTKRGRTSSSPSITGGRPWPSLLCTWAPA
CP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001161428
ORF Size 1266 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001161428.1, NP_001154900.1
RefSeq Size 2003 bp
RefSeq ORF 1269 bp
Locus ID 54445
UniProt ID Q8VCW4
MW 48.3 kDa
Gene Summary Plays an important role in innate and adaptive immunity by regulating nucleotide-sensing Toll-like receptor (TLR) signaling. Required for the transport of a subset of TLRs (including TLR3, TLR7 and TLR9) from the endoplasmic reticulum to endolysosomes where they can engage pathogen nucleotides and activate signaling cascades. May play a role in autoreactive B-cells removal.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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