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Brsk2 (NM_029426) Mouse Tagged ORF Clone
CAT＃: MR224913
- TrueORF®
Brsk2 (Myc-DDK-tagged) - Mouse BR serine/threonine kinase 2 (Brsk2), transcript variant 1
ORF Plasmid: tGFP
"NM_029426" in other vectors (4)
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CNY 6,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4833424K13Rik; SAD-A; SADA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224913 representing NM_029426
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCGACGGGGAAGGACGGCGGCGGGGCGCAGCACGCGCAGTATGTGGGGCCCTACCGGCTGGAGA AGACGCTGGGCAAGGGGCAGACAGGCTTGGTGAAGCTGGGAATCCACTGTGTCACTTGCCAGAAGGTCGC CATCAAAATCGTGAACCGTGAGAAGCTCAGTGAGTCGGTGCTGATGAAGGTGGAGCGAGAGATTGCCATC TTGAAGCTCATCGAGCATCCACATGTACTGAAGCTGCATGACGTCTATGAAAACAAAAAATATTTATACC TGGTGCTAGAACATGTGTCTGGGGGAGAGCTGTTCGACTACCTGGTGAAGAAGGGCCGGCTGACCCCCAA GGAGGCCCGCAAGTTCTTCCGGCAGATCATCTCTGCACTGGACTTCTGTCACAGCCACTCCATATGCCAT AGAGACTTGAAGCCAGAGAACCTGCTGCTAGATGAGAGGAACAACATCCGTATTGCAGACTTTGGCATGG CATCCCTGCAGGTGGGAGACAGCCTGCTGGAGACCAGCTGCGGATCTCCACACTATGCCTGTCCGGAAGT GATTCGGGGCGAGAAGTATGATGGCCGCAAGGCAGATGTGTGGAGCTGTGGTGTGATCCTGTTCGCCTTG CTGGTGGGGGCTCTGCCTTTTGATGATGACAACCTGCGGCAGTTGCTGGAGAAGGTCAAGCGTGGTGTGT TCCACATGCCACACTTTATCCCACCAGACTGCCAGAGTCTCCTGCGTGGCATGATTGAGGTGGATGCAGC TCGGCGCCTCACGCTAGAGCACATTCAGAAACACATATGGTATATAGGTGGCAAGAATGAGCCAGAGCCC GAACAGCCCATCCCACGCAAGGTGCAGATCCGCTCACTACCCAGCTTGGAAGACATTGACCCTGATGTGT TGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAAGCTGCTGCAGGATCTGCTATCTGAGGA GGAGAATCAGGAAAAGATGATTTATTTCCTCCTCCTGGATCGGAAAGAACGGTATCCAAGCCATGAGGAT GAGGACCTGCCCCCCAGGAATGAGATAGACCCTCCCCGGAAGCGTGTGGATTCCCCGATGCTGAACCGGC ATGGCAAGCGGCGACCTGAGCGCAAGTCCATGGAAGTGCTCAGTGTGACAGATGGTGGCTCCCCAGTGCC TGCACGGAGAGCCATTGAGATGGCCCAGCATGGCCAGAGATCTCGATCCATCAGTGGTGCGTCCTCAGGC CTTTCTACAAGTCCACTCAGCAGTCCTCGGGTGACCCCTCACCCCTCACCAAGGGGTAGTCCCCTTCCTA CCCCCAAAGGGACGCCTGTCCACACGCCAAAGGAGAGCCCAGCTGGCACACCCAACCCCACACCACCATC CAGCCCTAGTGTTGGAGGAGTGCCCTGGCGGACACGACTGAACTCCATCAAGAACAGCTTCCTGGGCTCA CCTCGATTCCACCGCCGGAAACTCCAAGTTCCCACGCCAGAGGAGATGTCCAACCTGACCCCAGAATCCT CTCCAGAGCTGGCCAAGAAATCGTGGTTCGGGAACTTCATCAACCTGGAGAAGGAGGAGCAGATCTTTGT GGTGATCAAGGACAAGCCCCTGAGCTCCATCAAGGCTGACATCGTTCATGCCTTCCTGTCGATCCCCAGC CTCAGCCACAGCGTTATTTCCCAGACAAGCTTCAGGGCTGAATACAAGGCCACAGGGGGCCCAGCAGTGT TCCAGAAGCCGGTCAAGTTCCAGGTGGACATCACCTACACTGAGGGCGGAGAGGCCCAGAAGGAGAATGG CATCTACTCAGTCACATTCACTTTACTCTCAGGCCCCAGTCGCCGCTTCAAGAGGGTGGTGGAGACCATC CAGGCCCAGCTGTTAAGCACCCATGACCAGCCATCAGCCCAGCACCTGTCAGGAATTATCCCGAAAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224913 representing NM_029426
Red=Cloning site Green=Tags(s) MTSTGKDGGGAQHAQYVGPYRLEKTLGKGQTGLVKLGIHCVTCQKVAIKIVNREKLSESVLMKVEREIAI LKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICH RDLKPENLLLDERNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFAL LVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEP EQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSHED EDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSG LSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRTRLNSIKNSFLGS PRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPS LSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETI QAQLLSTHDQPSAQHLSGIIPKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029426 |
| ORF Size | 1959 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029426.2, NP_083702.1 |
| RefSeq Size | 4047 bp |
| RefSeq ORF | 1962 bp |
| Locus ID | 75770 |
| UniProt ID | Q69Z98 |
| MW | 73.6 kDa |
| Gene Summary | Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-175 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-261 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220106 | Brsk2 (untagged) - Mouse BR serine/threonine kinase 2 (Brsk2), transcript variant 1, (10ug) |
CNY 8,360.00 |
|
| MG224913 | Brsk2 (tGFP-tagged) - Mouse BR serine/threonine kinase 2 (Brsk2) transcript variant 1, (10ug) |
CNY 7,220.00 |
|
| MR224913L3 | Lenti ORF clone of Brsk2 (Myc-DDK-tagged) - Mouse BR serine/threonine kinase 2 (Brsk2), transcript variant 1 |
CNY 8,460.00 |
|
| MR224913L4 | Lenti ORF clone of Brsk2 (mGFP-tagged) - Mouse BR serine/threonine kinase 2 (Brsk2), transcript variant 1 |
CNY 8,460.00 |
