Xpo5 (NM_028198) Mouse Tagged ORF Clone
CAT#: MR225052
- TrueORF®
Xpo5 (Myc-DDK-tagged) - Mouse exportin 5 (Xpo5)
ORF Plasmid: tGFP
"NM_028198" in other vectors (4)
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CNY 11,970.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2410004H11Rik; 2700038C24Rik; AI648907; AW549301; Exp5; mKIAA1291; RanBp21 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225052 representing NM_028198
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATGGAGCAAGTGAACGCGCTGTGCGAGGAGCTAGTGAAAGCGGTGACGGTCATGATGGACCCCA GCTCTACCCAGCGCTACCGGCTGGAAGCCCTCAAGTTTTGTGAAGAGTTTAAAGAAAAATGCCCTATCTG TGTTCCATGTGGCTTGAAGTTGGCTGAAAAAACACAAATTGCCATCGTCAGACATTTTGGCCTTCAGATC CTGGAGCATGTTGTCAAGTTTCGATGGAACAGCATGTCCCGATTGGAGAAGGTCTATCTGAAGAACAGTG TCATGGAGCTGATCGCAAATGGAACACTGAGGATTTTGGAGGAGGAGAACCACATTAAAGATGTTCTGTC TAGAATTGTGGTAGAGATGATCAAACGAGAGTGGCCACAGCACTGGCCTGACATGCTCATGGAGTTGGAT ACTCTCTTCAGGCAAGGGGAAACGCAGAGGGAGTTGGTGATGTTCATCCTTCTCCGACTGGCCGAGGATG TAGTGACCTTTCAGACGCTCCCCACTCAAAGAAGAAGGGATATTCAGCAAACATTGACGCAGAACATGGA AAGAATCTTGAATTTTCTACTCAATACGCTTCAGGAAAATGTAAACAAGTACCAACAAATGAAGACAGAT TCATCTCAGGAGGCAGAGGCTCAAGCCAACTGTCGAGTAAGCGTGGCGGCCCTGAACACTCTAGCCGGCT ACATTGACTGGGTATCTTTGAACCATATCACTGCCGAGAACTGTAAACTCGTGGAGACTCTGTGCCTGCT TCTCAATGAGCAGGAGCTGCAGTTGGGAGCCGCCGAGTGTCTGCTCATTGCAGTCAGCAGAAAAGGCAAG CTGGAGGACCGGAAGCGCTTGATGATCCTGTTTGGAGATGTCGCCATGCATTATATTCTCTCGGCCGCAC AGACTGCAGATGGAGGAGGCTTGGTCGAAAAACACTACCTCTTCCTGAAGAGACTTTGTCAGGTGTTATG TGCACTGGGAAATCTGCTATGTGCACTGCTGGCCTTAGATGCTAACATACAAACACCTATAAACTTTGGA ATGTACCTGGAATCTTTTCTTGCTTTCACAACCCATCCAAGTCAGTTTTTACGCTCTTCAACCCATATGA CGTGGGGAGCCCTCTTCAGGCATGAAGTTCTGTCCCGTGATCCGGCACTGTTAGCAGTAATACCAAAATA TCTTCGTGCTTCTATGACAAACTTGGTCAAGATGGGCTTTCCTTCTAAAACAGATAGCCCCAGCTGTGAA TATTCCCGATTTGATTTCGATAGTGATGAGGACTTCAATGCTTTCTTCAACTCCTCCCGAGCCCAGCATG GAGAGGTGGTGAGGTGTGTGTGTCGACTGGATCCTAAGACTAGCTTCCAGATGGCGGCAGAATGGCTCAA GTATCAGCTGTCAGCTTCTATTGACACTGGACCTGTGAACTCATGTTCTACAGCTGGAACTGGAGAGGGA GGCTTCTGCTCCATCTTCTCACCTTCATATGTGCAGTGGGAAGCTATGACTTTTTTTTTGGAAAGTGTGA TCAACCAGATGTTTCGAACACTAGACAAAGAAGAGCTCCCGGTTAGTGATGGGATAGAGCTGCTGCAGCT GGTGTTGAACTTTGAAATCAAGGATCCCCTCGTCCTGTCCTGCGTCCTCACTAATGTCTCAGCGCTCTTC CCATTTGTCACATACAAGCCTGCGTTCTTGCCCCAGGTCTTCTCTAAGCTCTTTTCATTTGTTACTTTTG AATCTGTTGGGGAAAGTAAGGCCCCCAGGACTCGGGCAGTCAGGAATGTAAGGAGACATGCTTGTTCCTC CATCAACAAGATGTGTCGGGACTACCCAGACCTTGTTCTGCCCAATTTTGACATGCTTTATAGCCACGTG AAGCAACTCCTCTCCAATGAGCTCCTCCTGACCCAGATGGAGAAGTGCGCCCTCATGGAAGCTCTGGTTC TCGTTAGCAACCAATTCAAGGATTATGAGCGGCAGAAGTTATTCCTAGAGGAGCTGATGGCACCGGTGGT CAACATCTGGCTTTCTGAAGAAATGTGCAGAGCGCTGTCAGATATTGACTCCTTCATTGCCTATGTGGGT GCTGATCTGAAAAGCTGTGACCCAGCTGTGGAGGATCCATGTGGCTTGAACCGTGCACGAATGAGCTTTT GTGTGTACAGCATCCTGGGGGTTATGAGACGCACTAGCTGGCCTTCCGACCTAGAAGAAGCCAAAGCTGG GGGCTTTGTGGTGGGCTACACACCCAGTGGAAATCCCATCTTCCGTAACCCCTGCACAGAGCAGATCCTC AGACTTCTCGACAATTTGCTTGCCCTTGTAAGAACTCACAATACTTTATATACACCGGAAATGCTAACGA AAATGGCAGAACCTTTCACCAAGGCTCTGGATATAGTTGAATCTGAAAAAACGGCAATATTAGGATTACC TCAGCCTCTCTTGGAATTCAACGACCACCCTGTCTATAGAACCACTCTGGAGAGGATGCAGCGGTTCTTT GGCATTCTCTATGAAAACTGTTACCATATCCTAGGGAAGGCAGGCCCTTCCATGCAGCAAGATTTCTACA CTGTGGAGGACCTTGCTTCCCAGCTCCTTGGATCAGCTTTCGTCAACTTGAACAATATTCCTGACTTCCG GCTTAGATCTATGCTTCGTGTCTTCGTGAAGCCCCTCGTGCTCTTCTGCCCCTCAGAACACTATGAAACC CTGATATCTCCCATCCTTGGACCTCTCTTCACCTACCTCCACATGAGGCTCTCTCAGAAGTGGCATGTCA TCAACCAGAGGAGCATCCTGTGTGGAGAAGATGAGATTGCAGAGGACAACCCCGAGTCTCAGGAAATGCT CGAGGAACAGCTGGTGAGGATGCTCACCCGAGAAGCCATGGACCTAATCATGGCTTGCTGTGTGTCGAAG AAGACTGCCGACCACACAGCCGCTCCCACTGCAGATGGAGATGATGAAGAGATGATGGCCACTGAAGTAG CCCCCTCGTCTGTGGTGGAGCTCACAGACCTGGGCAAATGCCTCATGAAGCACGAGGATGTCTGCACAGC ACTGCTAATCACAGCATTTAATTCTCTGACCTGGAAGGACACACTGTCTTGCCAGAGGGCTACCACACAG CTCTGCTGGCCCCTCCTCAAACAGGTGATGTCTGGGACCCTGCTCGCAGACGCTGTCACTTGGCTTTTCA CCAGTGTGCTGAAAGGACTGCAGATGCACGGGCAGCACGATGGGTGCATGGCTTCCCTGGTCCACTTGGC CTTCCAGATATACGAGGCGCTGCGCCCCAGGTACCTAGAGATAAGAGCAGTAATGGAGCAGATCCCTGAA ATAAACAAGGAGTCTCTGGACCAATTTGACTGCAAGCTTTTAAACCCCTCCCTTCAAAAAGCAGCTGATA AACGACGGAAGGACCACTTCAAACGTCTAATCGCTGGCTGCATTGGGAAACCCTTGGGAGAACAGTTCCG AAAAGAAGTTCACATTAAGAACCTTCCCTGGCTTTTCAAAAAACCCAAACCAATGTTGGAGACAGAAGTG CTGGACAGTGAGGAGGGTGGACTGGCCACCATCTTTGAACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225052 representing NM_028198
Red=Cloning site Green=Tags(s) MEMEQVNALCEELVKAVTVMMDPSSTQRYRLEALKFCEEFKEKCPICVPCGLKLAEKTQIAIVRHFGLQI LEHVVKFRWNSMSRLEKVYLKNSVMELIANGTLRILEEENHIKDVLSRIVVEMIKREWPQHWPDMLMELD TLFRQGETQRELVMFILLRLAEDVVTFQTLPTQRRRDIQQTLTQNMERILNFLLNTLQENVNKYQQMKTD SSQEAEAQANCRVSVAALNTLAGYIDWVSLNHITAENCKLVETLCLLLNEQELQLGAAECLLIAVSRKGK LEDRKRLMILFGDVAMHYILSAAQTADGGGLVEKHYLFLKRLCQVLCALGNLLCALLALDANIQTPINFG MYLESFLAFTTHPSQFLRSSTHMTWGALFRHEVLSRDPALLAVIPKYLRASMTNLVKMGFPSKTDSPSCE YSRFDFDSDEDFNAFFNSSRAQHGEVVRCVCRLDPKTSFQMAAEWLKYQLSASIDTGPVNSCSTAGTGEG GFCSIFSPSYVQWEAMTFFLESVINQMFRTLDKEELPVSDGIELLQLVLNFEIKDPLVLSCVLTNVSALF PFVTYKPAFLPQVFSKLFSFVTFESVGESKAPRTRAVRNVRRHACSSINKMCRDYPDLVLPNFDMLYSHV KQLLSNELLLTQMEKCALMEALVLVSNQFKDYERQKLFLEELMAPVVNIWLSEEMCRALSDIDSFIAYVG ADLKSCDPAVEDPCGLNRARMSFCVYSILGVMRRTSWPSDLEEAKAGGFVVGYTPSGNPIFRNPCTEQIL RLLDNLLALVRTHNTLYTPEMLTKMAEPFTKALDIVESEKTAILGLPQPLLEFNDHPVYRTTLERMQRFF GILYENCYHILGKAGPSMQQDFYTVEDLASQLLGSAFVNLNNIPDFRLRSMLRVFVKPLVLFCPSEHYET LISPILGPLFTYLHMRLSQKWHVINQRSILCGEDEIAEDNPESQEMLEEQLVRMLTREAMDLIMACCVSK KTADHTAAPTADGDDEEMMATEVAPSSVVELTDLGKCLMKHEDVCTALLITAFNSLTWKDTLSCQRATTQ LCWPLLKQVMSGTLLADAVTWLFTSVLKGLQMHGQHDGCMASLVHLAFQIYEALRPRYLEIRAVMEQIPE INKESLDQFDCKLLNPSLQKAADKRRKDHFKRLIAGCIGKPLGEQFRKEVHIKNLPWLFKKPKPMLETEV LDSEEGGLATIFEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028198 |
ORF Size | 3612 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028198.3 |
RefSeq Size | 3709 bp |
RefSeq ORF | 3615 bp |
Locus ID | 72322 |
UniProt ID | Q924C1 |
MW | 137.4 kDa |
Gene Summary | Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi) (By similarity). Mediates nuclear export of ADAR/ADAR1 in a RanGTP-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223945 | Xpo5 (untagged) - Mouse exportin 5 (Xpo5), (10ug) |
CNY 15,490.00 |
|
MG225052 | Xpo5 (tGFP-tagged) - Mouse exportin 5 (Xpo5), (10ug) |
CNY 13,210.00 |
|
MR225052L3 | Lenti ORF clone of Xpo5 (Myc-DDK-tagged) - Mouse exportin 5 (Xpo5) |
CNY 13,870.00 |
|
MR225052L4 | Lenti ORF clone of Xpo5 (mGFP-tagged) - Mouse exportin 5 (Xpo5) |
CNY 13,870.00 |