Ebf1 (NM_007897) Mouse Tagged ORF Clone
CAT#: MR225312
- TrueORF®
Ebf1 (Myc-DDK-tagged) - Mouse early B-cell factor 1 (Ebf1)
ORF Plasmid: tGFP
"NM_007897" in other vectors (6)
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CNY 6,608.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Ebf; O/E-1; OE-1; Olf-1; Olf1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225312 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGGGATCCAGGAAAGCATCCAACGGAGTGGAAGCAGTATGAAGGAAGAGCCGCTGGGCAGCGGCA TGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACAGCGGCGCAGAGCGGGGT GGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCTAACCTGCGGAAATCCAACTTCTTCCACTTC GTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGTGGAGATTGAGAGGACTGCCTTTGTGGGGTTCGTGG AGAAGGAAAAAGAAGCCAACAGCGAAAAGACCAATAATGGGATCCACTACCGGCTCCAGCTCCTCTACAG CAATGGGATACGGACAGAACAGGATTTCTATGTGCGCCTCATCGACTCCATGACAAAACAAGCCATAGTG TATGAAGGCCAAGACAAGAACCCTGAAATGTGCCGAGTATTGCTCACACACGAGATCATGTGCAGCCGCT GTTGTGACAAGAAAAGCTGTGGCAACCGAAATGAGACTCCCTCAGATCCAGTGATAATTGACAGGTTCTT CCTGAAGTTTTTCCTTAAATGCAACCAAAATTGCCTAAAGAATGCAGGAAACCCACGTGACATGCGGAGA TTCCAGGTCGTGGTGTCTACCACAGTCAACGTGGATGGCCATGTCCTGGCAGTCTCTGATAACATGTTTG TCCACAATAACTCCAAGCACGGGCGGAGGGCTCGGAGGCTTGACCCCTCGGAAGGTACGCCCTCTTATCT GGAACATGCTACTCCCTGTATCAAAGCCATCAGCCCGAGTGAAGGATGGACGACGGGAGGCGCGACTGTG ATCATCATAGGGGACAATTTCTTTGATGGGTTACAGGTCATATTCGGTACCATGCTGGTCTGGAGTGAGT TGATAACTCCTCATGCCATCCGAGTTCAGACACCTCCTCGGCACATCCCTGGTGTGGTGGAAGTCACACT GTCGTACAAGTCCAAGCAGTTCTGCAAAGGGACACCAGGCAGATTCATCTACACAGCACTCAATGAACCC ACCATCGACTACGGCTTCCAGAGGTTACAGAAGGTCATTCCTCGGCATCCTGGTGACCCAGAGCGCTTGC CAAAGGAAGTGATCCTTAAAAGAGCTGCAGATCTGGTTGAAGCCCTGTATGGGATGCCCCACAACAACCA GGAGATTATCCTGAAGAGAGCTGCCGACATTGCAGAGGCTCTGTACAGTGTCCCTCGGAACCACAACCAG CTCCCAGCCCTTGCTAACACTTCGGTCCATGCAGGGATGATGGGTGTGAACTCCTTCAGTGGACAACTGG CTGTGAATGTCTCGGAGGCATCACAAGCCACCAATCAAGGTTTCACCCGCAACTCAAGCAGCGTGTCACC ACATGGCTATGTGCCGAGCACCACCCCACAGCAAACCAACTATAACTCAGTCACCACAAGCATGAATGGC TACGGCTCAGCCGCCATGTCCAATTTGGGCGGCTCCCCAACCTTCCTCAATGGCTCAGCTGCCAACTCAC CCTATGCCATTGTGCCATCCAGCCCCACCATGGCCTCATCTACAAGCCTCCCCTCCAACTGCAGTAGCTC CTCTGGCATCTTCTCCTTCTCACCAGCCAACATGGTCTCAGCAGTGAAACAGAAGAGTGCTTTCGCACCA GTTGTCAGACCCCAGACGTCCCCTCCTCCCACCTGCACCAGCACCAACGGGAACAGCCTGCAAGCGATAT CTGGCATGATTGTCCCTCCCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225312 protein sequence
Red=Cloning site Green=Tags(s) MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHF VLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIV YEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRR FQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATV IIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEP TIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQ LPALANTSVHAGMMGVNSFSGQLAVNVSEASQATNQGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNG YGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAP VVRPQTSPPPTCTSTNGNSLQAISGMIVPPM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007897 |
ORF Size | 1776 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007897.1 |
RefSeq Size | 5203 bp |
RefSeq ORF | 1776 bp |
Locus ID | 13591 |
UniProt ID | Q07802 |
MW | 64.5 kDa |
Gene Summary | Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219382 | Ebf1 (untagged) - Mouse early B-cell factor 1 (Ebf1), (10ug) |
CNY 7,510.00 |
|
MG225312 | Ebf1 (tGFP-tagged) - Mouse early B-cell factor 1 (Ebf1), (10ug) |
CNY 6,460.00 |
|
MR225312L1 | Lenti ORF clone of Ebf1 (Myc-DDK-tagged) - Mouse early B-cell factor 1 (Ebf1) |
CNY 9,008.00 |
|
MR225312L2 | Lenti ORF clone of Ebf1 (mGFP-tagged) - Mouse early B-cell factor 1 (Ebf1) |
CNY 7,790.00 |
|
MR225312L3 | Lenti ORF clone of Ebf1 (Myc-DDK-tagged) - Mouse early B-cell factor 1 (Ebf1) |
CNY 7,790.00 |
|
MR225312L4 | Lenti ORF clone of Ebf1 (mGFP-tagged) - Mouse early B-cell factor 1 (Ebf1) |
CNY 7,790.00 |