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Chrd (NM_009893) Mouse Tagged ORF Clone
CAT＃: MR225319
- TrueORF®
Chrd (Myc-DDK-tagged) - Mouse chordin (Chrd)
ORF Plasmid: tGFP
"NM_009893" in other vectors (4)
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CNY 6,944.00
CNY 8,360.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Chd |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225319 representing NM_009893
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGCCTCCCGGCCCCGCCGGCCCCGCGGCTGCTCCTCGGGCTGCTGCTGCTCGGCTCGCGGCCGG CCAGTGGCACTGGCCCTGAGCCCCCAGCACTGCCCATCCGTTCCGAGAAGGAGCCGCTGCCTGTCCGGGG AGCGGCAGGCTGCTCCTTCGGCGGGAAGGTCTATGCCTTGGACGAGACGTGGCACCCGGACCTGGGGGAG CCTTTTGGGGTGATGCGCTGCGTGCTGTGCGCCTGTGAAGCGCCTCAGTGGGCTCGCCGTGGGAGGGGTC CTGGCAGGGTCAGCTGCAAGAACATCAAACCTCAGTGCCCCACCCTGGCCTGCAGGCAGCCGCGCCAGCT GCCAGGACACTGCTGCCAGACCTGCCCGCAGGAGCGCAGCAATCTAGATCCACAGCCCGCTGGCCTGGTC TTCGAGTATCCAAGGGATCCAGAGCATCGCAGTTACAGTGATCGAGGGGAACCCGGCGTTGGGGAGCGGA CCCGTGCTGATGGCCACACAGACTTTGTGGCGCTGCTGACAGGACCGAGGTCGCAGGCGGTAGCTCGTGC TCGAGTCTCTTTGCTGCGCTCAAGTTTACGCTTCTCTGTCTCCTACCAGCGGCTGGACCGTCCCAGCAGG GTTCGTTTCACAGATCCCACAGGCAACATCCTGTTTGAACACCCTGCAACCCCCACCCAGGATGGCCTGG TCTGTGGGGTGTGGCGGGCAGTGCCTCGGCTGTCTGTGAGGCTCCTGAGGGCAGAGCAGCTTCGGGTAGC CCTTGTGACATCCACTCACCCTTCAGGAGAAGTCTGGGGGCCTCTCATTTGGCAGGGTGCTCTCGCTGCA GAGACCTTCAGTGCCATCCTGACCCTGGAAGACCCACTGCAGCGGGGTGTGGGGGGCATAGCCCTGCTCA CCCTCAGTGACACAGAAGATTCCTTGCATTTTTTGCTGCTCTTCCGGGGTCTGCTGGGAGGACTAGCTCA GGCTCCCTTGAAGCTTCAGATTCTCCACCAGGGACAGCTACTTCGGGAGCTCCAGGCCAACACCTCCGCT CAGGAGCCAGGTTTTGCTGAGGTGCTGCCCAGCCTTACAGACCAAGAGATGGACTGGTTGGAGCTGGGGG AGCTGCAGATGGTCCTAGAGAAGGCGGGTGGGCCAGAGCTACGCATCAGTGGATACATCACCACCAGGCA GAGCTGTGATGTCCTTCAAAGTGTCCTTTGTGGGGCTGATGCCCTGATCCCAGTCCAGACGGGTGCTGCT GGGTCAGCCAGCTTCATATTGCTAGGAAATGGCTCCCTTATCTATCAGGTGCAAGTGGTAGGTACAGGTA GCGAGGTGGTGGCCATGACACTGGAGACCAAGCCTCAGCGGAAGAACCAGCGCACTGTCCTGTGCCACAT GGCTGGACTCCAGCCGGGAGGACACATGGCTGTGGGTATGTGCTCTGGGCTGGGTGCCCGAGGGGCTCAT ATGCTGCTACAGAACGAGCTGTTCCTGAATGTTGGTACCAAGGACTTCCCAGATGGAGAGCTTCGGGGGC ATGTGACTGCCCTATGCTACAGTGGGCACAGTGCCCGCTATGACAGATTGCCTGTGCCTCTGGCAGGGGC ACTAGTGCTGCCCCCTGTGCGGAGTCAGGCAGCAGGGCACGCCTGGCTCTCCTTGGATACACATTGTCAC TTACACTATGAAGTTCTATTGGCTGGGCTTGGTGGCTCAGAGCAAGGAACTGTCACTGCCCACCTCCTTG GGCCTCCTGGGATGCCAGGGCCCCAGCGGCTGCTGAAGGGATTCTATGGCTCAGAGGCTCAGGGCGTGGT AAAAGATCTGGAGCCTGTGCTGCTGCGGCACCTGGCACAGGGCACTGCCTCCCTGCTCATCACCACCAAG AGTAGCCCCAGAGGAGAACTACGTGGGCAGGTGCACATTGCCAGTCAGTGTGAGGCGGGAGGCCTGCGTC TGGCCTCAGAAGGAGTGCAGATGCCCTTGGCTCCAAATGGAGAGGCGGCTACGTCACCCATGCTGCCTGC TGGCCCTGGCCCTGAAGCCCCAGTACCAGCCAAACATGGCAGCCCTGGGAGACCCCGAGATCCTAACACA TGTTTCTTCGAGGGGCAGCAGCGGCCCCACGGGGCTCGCTGGGCACCCAACTATGACCCACTCTGCTCCC TCTGCATCTGCCAGAGACGAACAGTGATCTGTGACCCTGTAGTATGCCCACCACCAAGCTGTCCCCACCC GGTGCAGGCACTGGACCAGTGCTGTCCCGTGTGTCCAGAGAAACAACGCAGTAGAGACCTCCCCAGCCTA CCAAATCTGGAGCCAGGAGAGGGCTGCTATTTTGATGGTGACCGGAGCTGGAGGGCAGCGGGTACCCGAT GGCACCCTGTTGTGCCCCCCTTTGGCCTAATTAAGTGTGCTGTCTGCACCTGCAAGGGGGCCACGGGAGA GGTGCACTGTGAGAAGGTGCAGTGTCCTCGCCTGGCCTGTGCTCAGCCTGTCCGTGCCAACCCCACCGAC TGCTGCAAACAGTGTCCAGTAGGGTCAGGGACTAATGCCAAGCTGGGAGACCCCATGCAGGCTGATGGGC CTCGGGGGTGTCGCTTTGCTGGGCAGTGGTTCCCAGAGAATCAGAGCTGGCACCCATCAGTGCCCCCCTT TGGGGAGATGAGCTGTATTACCTGCAGATGTGGGGCAGGGGTACCCCACTGTGAGCGGGACGATTGTTCA CCGCCGCTATCCTGTGGCTCAGGGAAGGAGAGTCGATGCTGTTCCCACTGCACAGCCCAAAGGTCCTCTG AGACTAGAACCCTTCCAGAGCTGGAGAAAGAAGCGGAGCACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225319 representing NM_009893
Red=Cloning site Green=Tags(s) MPSLPAPPAPRLLLGLLLLGSRPASGTGPEPPALPIRSEKEPLPVRGAAGCSFGGKVYALDETWHPDLGE PFGVMRCVLCACEAPQWARRGRGPGRVSCKNIKPQCPTLACRQPRQLPGHCCQTCPQERSNLDPQPAGLV FEYPRDPEHRSYSDRGEPGVGERTRADGHTDFVALLTGPRSQAVARARVSLLRSSLRFSVSYQRLDRPSR VRFTDPTGNILFEHPATPTQDGLVCGVWRAVPRLSVRLLRAEQLRVALVTSTHPSGEVWGPLIWQGALAA ETFSAILTLEDPLQRGVGGIALLTLSDTEDSLHFLLLFRGLLGGLAQAPLKLQILHQGQLLRELQANTSA QEPGFAEVLPSLTDQEMDWLELGELQMVLEKAGGPELRISGYITTRQSCDVLQSVLCGADALIPVQTGAA GSASFILLGNGSLIYQVQVVGTGSEVVAMTLETKPQRKNQRTVLCHMAGLQPGGHMAVGMCSGLGARGAH MLLQNELFLNVGTKDFPDGELRGHVTALCYSGHSARYDRLPVPLAGALVLPPVRSQAAGHAWLSLDTHCH LHYEVLLAGLGGSEQGTVTAHLLGPPGMPGPQRLLKGFYGSEAQGVVKDLEPVLLRHLAQGTASLLITTK SSPRGELRGQVHIASQCEAGGLRLASEGVQMPLAPNGEAATSPMLPAGPGPEAPVPAKHGSPGRPRDPNT CFFEGQQRPHGARWAPNYDPLCSLCICQRRTVICDPVVCPPPSCPHPVQALDQCCPVCPEKQRSRDLPSL PNLEPGEGCYFDGDRSWRAAGTRWHPVVPPFGLIKCAVCTCKGATGEVHCEKVQCPRLACAQPVRANPTD CCKQCPVGSGTNAKLGDPMQADGPRGCRFAGQWFPENQSWHPSVPPFGEMSCITCRCGAGVPHCERDDCS PPLSCGSGKESRCCSHCTAQRSSETRTLPELEKEAEHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009893 |
| ORF Size | 2844 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009893.2, NP_034023.1 |
| RefSeq Size | 3292 bp |
| RefSeq ORF | 2847 bp |
| Locus ID | 12667 |
| UniProt ID | Q9Z0E2 |
| MW | 102 kDa |
| Gene Summary | Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222772 | Chrd (untagged) - Mouse chordin (Chrd), (10ug) |
CNY 12,160.00 |
|
| MG225319 | Chrd (tGFP-tagged) - Mouse chordin (Chrd), (10ug) |
CNY 9,220.00 |
|
| MR225319L3 | Lenti ORF clone of Chrd (Myc-DDK-tagged) - Mouse chordin (Chrd) |
CNY 10,260.00 |
|
| MR225319L4 | Lenti ORF clone of Chrd (mGFP-tagged) - Mouse chordin (Chrd) |
CNY 10,260.00 |
