Lin28b (NM_001031772) Mouse Tagged Lenti ORF Clone
CAT#: MR225677L3
- LentiORF®
Lenti ORF clone of Lin28b (Myc-DDK-tagged) - Mouse lin-28 homolog B (C. elegans) (Lin28b)
"NM_001031772" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,800.00
CNY 600.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2810403D23Rik; D030047M17Rik; Lin-28.2 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR225677).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001031772 |
ORF Size | 813 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001031772.2, NP_001026942.1 |
RefSeq Size | 5420 bp |
RefSeq ORF | 816 bp |
Locus ID | 380669 |
UniProt ID | Q45KJ6 |
Gene Summary | Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop (By similarity). Mediates MYC-induced let-7 repression (PubMed:19211792). When overexpressed, may stimulate growth of carcinoma cell lines (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214667 | Lin28b (untagged) - Mouse lin-28 homolog B (C. elegans) (Lin28b), (10ug) |
CNY 2,400.00 |
|
MG225677 | Lin28b (tGFP-tagged) - Mouse lin-28 homolog B (C. elegans) (Lin28b), (10ug) |
CNY 4,000.00 |
|
MR225677 | Lin28b (Myc-DDK-tagged) - Mouse lin-28 homolog B (C. elegans) (Lin28b) |
CNY 2,400.00 |
|
MR225677L4 | Lenti ORF clone of Lin28b (mGFP-tagged) - Mouse lin-28 homolog B (C. elegans) (Lin28b) |
CNY 4,800.00 |