Pura (NM_008989) Mouse Tagged ORF Clone
CAT#: MR225884
- TrueORF®
Pura (Myc-DDK-tagged) - Mouse purine rich element binding protein A (Pura)
ORF Plasmid: tGFP
"NM_008989" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CAGER-1; Pur-alpha; ssCRE-BP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225884 representing NM_008989
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCGAGACAGCGGCAGCGAGCAGGGTGGTGCGGCGCTGGGCTCGGGCGGCTCCCTAGGGCACC CGGGCTCGGGCTCAGGCTCCGGCGGGGGCGGTGGTGGCGGCGGGGGCGGCGGCGGCAGTGGCGGCGGCGG CGGGGCCCCGGGGGGGCTGCAGCACGAGACGCAGGAGCTGGCCTCCAAGCGGGTGGACATCCAGAACAAG CGTTTCTACCTGGACGTGAAGCAGAACGCTAAGGGCCGTTTCCTGAAGATCGCAGAGGTGGGCGCTGGCG GCAACAAGAGCCGCCTCACCCTCTCCATGTCTGTGGCCGTGGAGTTCCGCGACTACCTGGGCGACTTCAT CGAGCACTACGCGCAGCTGGGCCCCAGCCAGCCACCCGACCTGGCCCAGGCACAGGACGAGCCACGCCGG GCGCTCAAGAGCGAGTTCCTGGTGCGCGAAAACCGCAAGTACTACATGGATCTCAAGGAGAACCAGCGCG GCCGCTTCCTGCGCATCCGCCAGACAGTCAACCGGGGGCCCGGCCTGGGCTCCACGCAGGGCCAGACCAT TGCGCTGCCCGCACAGGGTCTCATCGAGTTCCGTGACGCTCTGGCCAAGCTCATCGACGACTATGGAGTG GAGGAGGAGCCGGCCGAGCTGCCCGAGGGCACCTCCTTGACTGTGGACAACAAGCGCTTCTTCTTCGATG TGGGTTCCAACAAGTACGGCGTGTTTATGCGAGTGAGTGAGGTGAAGCCCACCTACCGCAACTCCATCAC CGTGCCCTACAAGGTGTGGGCCAAGTTCGGACACACCTTCTGCAAGTACTCCGAGGAGATGAAGAAGATT CAAGAGAAACAGAGGGAGAAGCGGGCCGCTTGTGAGCAGCTCCACCAGCAGCAACAGCAGCAGCAAGAGG AGACCACCGCTGCCACCCTGCTACTGCAGGGCGAGGAAGAAGGGGAAGAAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225884 representing NM_008989
Red=Cloning site Green=Tags(s) MADRDSGSEQGGAALGSGGSLGHPGSGSGSGGGGGGGGGGGGSGGGGGAPGGLQHETQELASKRVDIQNK RFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDLAQAQDEPRR ALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGV EEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPYKVWAKFGHTFCKYSEEMKKI QEKQREKRAACEQLHQQQQQQQEETTAATLLLQGEEEGEED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008989 |
ORF Size | 963 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008989.4 |
RefSeq Size | 1602 bp |
RefSeq ORF | 966 bp |
Locus ID | 19290 |
UniProt ID | P42669 |
MW | 35.3 kDa |
Gene Summary | This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the c-Myc gene. May play a role in the initiation of DNA replication and in recombination.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209266 | Pura (untagged) - Mouse purine rich element binding protein A (Pura), (10ug) |
CNY 2,400.00 |
|
MG225884 | Pura (tGFP-tagged) - Mouse purine rich element binding protein A (Pura), (10ug) |
CNY 2,850.00 |
|
MR225884L3 | Lenti ORF clone of Pura (Myc-DDK-tagged) - Mouse purine rich element binding protein A (Pura) |
CNY 4,750.00 |
|
MR225884L4 | Lenti ORF clone of Pura (mGFP-tagged) - Mouse purine rich element binding protein A (Pura) |
CNY 4,750.00 |