Ank2 (NM_001034168) Mouse Tagged ORF Clone
CAT#: MR226071
- TrueORF®
Ank2 (Myc-DDK-tagged) - Mouse ankyrin 2, brain (Ank2), transcript variant 3
ORF Plasmid: tGFP
"NM_001034168" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,310.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 100043364; AI835472; Ank-2; AW491075; Gm4392 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226071 representing NM_001034168
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGACAGGCAGTCACAGGGCACCACTCCTGATACCACTCCTGCTAGGACCCCAACTGAAGAGGGGA CTCCGACAAGTGAACAAAATCCATTCCTCTTTCAGGAAGGAAAACTGTTTGAGATGACCCGAAGTGGTGC TATCGATATGACCAAAAGGCCCTATGCAGATGAAAGTTTGCACTTTTTCCAAATCGGCCAAGAGTCCAAT GAAGAGGCTATCTCCGAAGACTTGAAGGAAGGGGCCACTGGGGCTGAGCCTCCACAGACGGAGACTACTA GTGAGTCGCTGGAACTTTCAGAACCCAAAGAAGCAATGGATGACGAAGGAGAATTACTTCCTGATGATGT AAGTGAGGAGATAGAGGATTTACCTGCCTCGGATGCTAACATTGACTCCCAAGTGATAATTTCAGCTTCC ACAGAAACACCCACCAAAGAGGCTGTATCCACAGCGGTCGAGGAGCCCCCCACCACACAGCGGAGTGATT CTCTGAGCACTGTGAAGCAGACACCACGCCCTGCCGTCCCTGGACCTGTTGGTCAGTTGGACTTTTCCCC CGTCACTAGGTCTGTTTATTCGGGACAGGATGATGAGTCCCCAGAGTCTTCTCCAGAGGAACAGAAGTCT GTGATTGAGATCCCTACTGCACCCGTGGACAACGTGCCTTCTGCCGAAAGCAAACCCCAAATTCCTATCA GGACTCTCCCCACTTTAGTCCCAGCCCCTCCATCTGCAGAGGATGAGAGTGCATTTTCTGATGATTTCCC ATCTAGCCTGGATGAGGATAGTAAGGAAGGTGGAGCAAAACCAAAGTCCAAAATTCCCGTCAAAGCACCC ACCCAAAGAACTGAGTGGCAGCCCTCCCCTACCGACATACCTCTCCAGAAGACAGCTGTCCCCCAGGGAC AGGAAACACTAAGCAGAGCACCAGATGGTAGAAGCAAGTCAGAGTCAGACGCTAGTTCCTTAGATGCTAA GACCAAATGCCCAGTGAAAGCCAGAAGTTACATTGAGACAGAGACGGAGAGCAGGGAGAGGGCCGAGGGG TTTGAGTCAGAATCAGAAGACGGGGCCACAAAACCAAAGCTCTTTGCATCCCGACTGCCGGTGAAGAGCA GGAGCACTTCATCTTCCGGCAGGCCAGGCACGAGCCCCACCAGAGAGAGCAGGGAGCACTTCTTTGACCT TTACCGAAACTCCATAGAATTCTTTGAGGAGATTAGTGATGAAGCTTCCAAATTAGTGGACAGGCTTACA CAGTCAGAGAGGGAGCAGGAGCCACCTTCAGACGACGAAAGTAGCAGTGCCCTGGAAGTGTCAGTCATTG AGAGTCTGCCACCTGTTGACATTGAGCACTCAGCTCCCGAGGACATCTTTGACACAAGGCCCATTTGGGA TGAGTCTATTGAGACTATGATTGAACGCATCCCTGATGAAAATGGCCATGACCGAGCTGAAGATCCCCAA GATGAGCAGGAGCGGATGGAAGAAAGGCTGGCTTACATCGCTGATCACCTTGGCTTCAGCTGGACAGAAT TAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCACCAAATTCGAATCGAGAACCCCAACTCCCTTCA AGATCAGAGCCACGCACTGCTCAAGTACTGGCTGGAGAGGGATGGGAAGCATGCCACAGATACCATCCTC ATCGAATGCCTCACCAAGATCAACAGGATGGACATTGTACATCTCCTGGAGACCAACACAGAGCCCCTCC AGGAGCGCATGGGCCGCAGCTATGCAGAAATAGAGCAGACCATTACGCTGGACCACAGTGAAGGATTTTC AGTCCTTCCAGACGAGCTCTGTGCTGCCAAGGAGAAGAAGGAGCAGGAAGCTTCCAAAGAAAGCGAGTCT AGCGACCACCCGCCCATGGTCTCCGAAGAAGACATATCTGTCGGTTATTCCACATTTCAGGATTGCCTCC CCAAAACTGAAGGGGACAGCCCAGCAGCAGCACTGTCTCCTCAAATGCACCAGGAGCCAGTTCAACAAGA TTTCTCAGGGAAAACGCAAGACCAGCAGGAATATTATGTAACAACACCAGGGGCAGAAGTGGAAGACCCT CAGAAGGCCACAGCCGTTCCTGACTCTCTCTGTAAGACTCCTGAGGACATCAGTACCCCTCCTGAGGGAA CAAAGCCTTGTCTCCAGACCCCGGTGACTAGCGAACGTGGTTCTCCGATTGTGCAAGAACCTGAGGAGGC TTCCGAGCCCAAAGAGGAGAGTTCTCCAAGGAAAACTAGCCTGGTCATTGTTGAGTCAACAGACGACCAG TCTCAGGTCTTTGAAAGACTGGATGGTGATGCCGCTTTTCAAAAGGGAGACGATATGCCTGACATACCCC CAGAGACGGTCACAGAGGAAGAATATGTTGATGAGAATGGACACACCGTGGTGAAGAAGGTTACCCGGAA AATCATTAGGCGGTACGTTTCCTCTGATGGCACAGAGAAGGAGGAGGTTACCATGCAGGGAATGCCTCAG GAGCCAGTCAACATTGAGGATGGGGACAATTATTCCAAAGTGATAAAGCGCGTGGTATTGAAGAGTGACA CCCAGCAGTCAGAGGTGACTTTGTCTGAACCCAGCGTTTTGTCCAGTACCTCACAGTTTCAGGCCGAGCC AGTAGAAGGCCGTAGAGTCAGCAAAGTTGTTAAAACAACCATGGTACACGGAGAACGGATGGAGAAGAGT CTTGGGGACTCTAGCTTAGCCACTGACCTTCCTTCAGCCAAAGATGACTTTGAAGAGGACAACAATGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226071 representing NM_001034168
Red=Cloning site Green=Tags(s) MVDRQSQGTTPDTTPARTPTEEGTPTSEQNPFLFQEGKLFEMTRSGAIDMTKRPYADESLHFFQIGQESN EEAISEDLKEGATGAEPPQTETTSESLELSEPKEAMDDEGELLPDDVSEEIEDLPASDANIDSQVIISAS TETPTKEAVSTAVEEPPTTQRSDSLSTVKQTPRPAVPGPVGQLDFSPVTRSVYSGQDDESPESSPEEQKS VIEIPTAPVDNVPSAESKPQIPIRTLPTLVPAPPSAEDESAFSDDFPSSLDEDSKEGGAKPKSKIPVKAP TQRTEWQPSPTDIPLQKTAVPQGQETLSRAPDGRSKSESDASSLDAKTKCPVKARSYIETETESRERAEG FESESEDGATKPKLFASRLPVKSRSTSSSGRPGTSPTRESREHFFDLYRNSIEFFEEISDEASKLVDRLT QSEREQEPPSDDESSSALEVSVIESLPPVDIEHSAPEDIFDTRPIWDESIETMIERIPDENGHDRAEDPQ DEQERMEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTIL IECLTKINRMDIVHLLETNTEPLQERMGRSYAEIEQTITLDHSEGFSVLPDELCAAKEKKEQEASKESES SDHPPMVSEEDISVGYSTFQDCLPKTEGDSPAAALSPQMHQEPVQQDFSGKTQDQQEYYVTTPGAEVEDP QKATAVPDSLCKTPEDISTPPEGTKPCLQTPVTSERGSPIVQEPEEASEPKEESSPRKTSLVIVESTDDQ SQVFERLDGDAAFQKGDDMPDIPPETVTEEEYVDENGHTVVKKVTRKIIRRYVSSDGTEKEEVTMQGMPQ EPVNIEDGDNYSKVIKRVVLKSDTQQSEVTLSEPSVLSSTSQFQAEPVEGRRVSKVVKTTMVHGERMEKS LGDSSLATDLPSAKDDFEEDNNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001034168 |
ORF Size | 2799 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001034168.1, NP_001029340.1 |
RefSeq Size | 5487 bp |
RefSeq ORF | 2802 bp |
Locus ID | 109676 |
UniProt ID | Q8C8R3 |
MW | 103.7 kDa |
Gene Summary | Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions (PubMed:19109891). In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites (PubMed:12571597). Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:15262991). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (PubMed:19007774). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (PubMed:27718357).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222704 | Ank2 (untagged) - Mouse ankyrin 2, brain (Ank2), transcript variant 3, (10ug) |
CNY 11,970.00 |
|
MG226071 | Ank2 (tGFP-tagged) - Mouse ankyrin 2 brain (Ank2) transcript variant 3, (10ug) |
CNY 10,260.00 |
|
MR226071L3 | Lenti ORF clone of Ank2 (Myc-DDK-tagged) - Mouse ankyrin 2, brain (Ank2), transcript variant 3 |
CNY 11,210.00 |
|
MR226071L4 | Lenti ORF clone of Ank2 (mGFP-tagged) - Mouse ankyrin 2, brain (Ank2), transcript variant 3 |
CNY 11,210.00 |