Spint2 (NM_011464) Mouse Tagged ORF Clone

CAT#: MR226284

  • TrueORF®

Spint2 (Myc-DDK-tagged) - Mouse serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_011464" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AL024025; C76321; HAI-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR226284 representing NM_011464
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCAGCTTTGTGAGCTGCGGCGGGGCCGGGCGCTCCTCGCCTTGGTAGCTTCGCTGCTCCTCTCCG
GGGCCCAGGTGGCCAGCCGAGAACTAGACGTCCACGAGTCTTGTGGAGTTTCGAAGGTAGTGGGGAAATG
CCGGGCTTCCATCCCCAGGTGGTGGTACAATATCACTGATGGGTCCTGCCAGCCATTTGTTTATGGAGGC
TGTGAAGGCAATGGCAATAACTACCAGAGCAAGGAGGAGTGTCTTGACAAGTGTGCTGGTGTCACTGAGA
ACACCACTGATGACATGGCCAGGAACAGGAATGGAGCCGACTCTTCTGTCCTGAGTGTTCCCAGAAAGCA
GAGTGCTGAGGACCTGTCTGCTGAGATCTTCAACTATGAAGAATACTGTGTCCCGAAGGCCGTCACTGGG
CCGTGCCGCGCAGCCTTCCCTCGCTGGTACTATGACACTGAAAAGAATTCCTGTATCAGCTTCATCTACG
GCGGGTGCAGAGGCAACAAGAACAGTTACCTCTCCCAGGAAGCATGCATGCAGCACTGCTCTGGTAAGCA
GATGCATCCTTTCCTGACCCCGGGCCTAAAAGCGGTGATCCTGGTGGGGCTGTTCCTCATGGTTCTGATC
CTCCTCCTGGGAACCTCTATGGTCTGCCTCATCCGTGTGGTTCGCAGAAAGCAGGAGCGTGCGCTGCGCA
CTGTTTGGAGCACTGCGGATGACAAGGAGCAGCTGGTGAAGAACACCTGTGTCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR226284 representing NM_011464
Red=Cloning site Green=Tags(s)

MAQLCELRRGRALLALVASLLLSGAQVASRELDVHESCGVSKVVGKCRASIPRWWYNITDGSCQPFVYGG
CEGNGNNYQSKEECLDKCAGVTENTTDDMARNRNGADSSVLSVPRKQSAEDLSAEIFNYEEYCVPKAVTG
PCRAAFPRWYYDTEKNSCISFIYGGCRGNKNSYLSQEACMQHCSGKQMHPFLTPGLKAVILVGLFLMVLI
LLLGTSMVCLIRVVRRKQERALRTVWSTADDKEQLVKNTCVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011464
ORF Size 756 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011464.2, NP_035594.1
RefSeq Size 1477 bp
RefSeq ORF 759 bp
Locus ID 20733
UniProt ID Q9WU03
MW 28.4 kDa
Gene Summary Inhibitor of HGF activator.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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