Plxna4 (NM_175750) Mouse Tagged ORF Clone
CAT#: MR226328
- TrueORF®
Plxna4 (Myc-DDK-tagged) - Mouse plexin A4 (Plxna4)
ORF Plasmid: tGFP
"NM_175750" in other vectors (2)
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CNY 19,056.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 9330117B14; mKIAA1550; Plxa4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226328 representing NM_175750
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGAACTGGACTTGCCTGCTCTCCCACCTGCTGGTGGTAGGGATGGGCTCCTCCACTCTGCTTC CACGACAGCCACCCCAGCTGTCTCAGAAGCCTTCCTTTGTGACATTCCGAGGGGAGCCTGCTGAGGGCTT CAATCACCTTGTGGTGGATGAGAGGACAGGGCACATTTATTTGGGGGCTGTCAACAGAATCTACAAACTC TCTAGTGACCTCAAGGTCTTGGTGACTCATCAGACAGGGCCAGATGAGGACAACCCCAAGTGTTACCCAC CTCGGATTGTTCAGACCTGCAATGAGCCTCTGGCCAGCACCAATAACGTCAACAAGATGCTGCTTATAGA CTACAAAGAAAACAGGCTGATCGCGTGTGGGAGCCTGTACCAGGGTATCTGCAAGCTCCTGAGGCTAGAG GACCTCTTCAAGTTGGGAGAGCCCTTTCACAAGAAGGAACATTATCTCTCGGGAGTTAACGAGAGTGGCT CAGTCTTTGGGGTGATTGTTTCCTACAGCAACTTTGATGACAAACTCTTTATTGCCACAGCAGTGGATGG CAAACCTGAATATTTTCCTACCATCTCCAGCAGGAAGCTGACTAAGAACTCTGAGGCCGATGGCATGTTT GCTTACGTTTTCCACGATGAATTTGTGGCCTCTATGATTAAGATCCCTTCAGACACTTTCACGGTTATCC CCGACTTTGACATCTACTACGTCTATGGCTTCAGCAGTGGCAATTTTGTCTACTTTTTGACCCTTCAGCC AGAGATGGTGTCTCCCCCAGGCTCCACGACAAAGGAGCAGGTGTACACCTCAAAGCTTGTGAGGCTTTGC AAAGAGGACACAGCCTTCAACTCCTACGTAGAGGTGCCCATTGGCTGTGAGCGCAATGGGGTGGAGTATC GCTTGTTACAGGCTGCCTACTTGTCTAAAGCTGGTGCAGTGCTTGGCCGGACCCTAGGAGTCCGCCCAGA TGACGACCTCCTCTTCACCGTCTTCTCCAAGGGCCAGAAGCGGAAGATGAAATCTCTGGATGAGTCTGCC CTGTGCATCTTCATCTTGAAGCAGATCAATGATCGCATTAAGGACCGCCTGCAGTCCTGTTATAGGGGTG AGGGAACACTAGACCTGGCCTGGCTCAAGGTGAAGGACATCCCTTGCAGCAGTGCGCTCCTTACTATTGA TGACAACTTCTGTGGCCTGGATATGAATGCTCCTCTGGGAGTTTCCGAAATGGTGCGTGGCATTCCAGTC TTCACAGAAGACAGGGACCGCATGACTTCTGTCATCGCCTATGTCTACAAGAACCACTCACTGGCCTTCG TGGGCACCAAAAGTGGCAAGCTGAAGAAGATCCGGGTTGATGGGCCCAAGGGAAATGCCCTCCAATATGA GACTGTGCAGGTGGTGGACTCAGGACCAGTCCTCCGGGACATGGCCTTTTCCAAGGACCACGAGCAACTC TACATCATGTCAGAAAGGCAGCTTACCAGAGTTCCTGTGGAGTCCTGTGGGCAGTACCGGAGCTGTGGCG AGTGTCTTGGCTCAGGTGACCCCCATTGTGGCTGGTGTGTGCTCCACAACACGTGCACCCGGAAGGAACG GTGTGAACGCTCCAGAGAACCCCGAAGGTTTGCCTCAGAGATGAAGCAGTGTGTCCGGCTGACGGTCCAT CCCAACAACATCTCTGTCTCTCAGTACAACGTGCTGCTGGTCCTAGAGACATACAATGTCCCGGAGCTGT CAGCAGGTGTCAACTGTACCTTTGAAGATCTGTCAGAGATGGATGGGCTGGTAATAGGCAATCAGATCCA GTGCTACTCCCCTGCAGCCAAGGAAGTGCCCCGGATCATCACAGAAAATGGGGACCATCATGTTGTCCAG CTGCAACTCAAGTCAAAGGAGACTGGGATGACCTTCGCCAGCACCAGCTTTGTCTTCTACAACTGCAGTG TCCACAATTCATGCCTGTCCTGTGTGGAGAGCCCGTACCGTTGCCACTGGTGTAAATACCGGCATGTTTG TACTCACGACCCCAACACATGCTCCTTCCAGGAAGGCCGAGTGAAGCTACCTGAGGACTGCCCCCAGCTG CTGCGAGTGGACAAGATCCTCGTTCCAGTAGAGGTGATCAAGCCCATCACTCTGAAGGCTAAGAACCTCC CACAGCCTCAGTCGGGACAGAGAGGCTACGAGTGCATCTTGAACATCCAGGGCATCGAGCAGAGGGTACC TGCTCTGCGCTTCAACAGCTCCAGTGTGCAGTGTCAGAACACCTCCTATTCCTATGAAGGAATGGAGATC AACAACCTGCCAGTGGAGTTGACAGTCGTGTGGAATGGACACTTCAACATTGACAACCCAGCTCAGAATA AAGTTTACCTCTACAAGTGTGGAGCCATGCGCGAGAGCTGTGGGCTGTGCCTCAAGGCCGACCCGGATTT CGAGTGTGGCTGGTGTCAGAGCCCAGGCCAGTGTACCCTGCGCCAGCACTGCCCTGCCCATGAGAGCCGG TGGTTGGAACTGTCGGGGGCCAACAGCAAGTGCACCAACCCTCGTATCACAGAGATCATCCCAGTGACGG GCCCTCGAGAAGGGGGCACCAAGGTCACCATCCGAGGGGAGAACTTGGGCCTGGAATTCCGTGACATTGC ATCACACGTGAAAGTGGCTGGTGTGGAATGCAGCCCCTTGGTGGATGGCTACATCCCAGCTGAACAGATC GTGTGTGAGATGGGTGAGGCCAAGCCTAGCCAGCATGCAGGCTTTGTGGAGATCTGTGTGGCTGTGTGTC GGCCTGAGTTCATGGCCCGCTCTTCGCAGCTCTATTACTTCATGACTCTGACCCTCGCAGATCTGAAGCC CAACCGAGGACCCATGTCTGGGGGGACACAGGTTACCATCACAGGTACCAACTTGAATGCAGGCAGCAAT GTTGTGGTGATGTTCGGGAGCCAGCCCTGCCTCTTCCACAGGCGCTCTCCATCCTACATTATCTGTAACA CTACATCCTCAGAGGAGGTGCTGGACATGAAGGTGACTGTGCAGGTGGACAGGGCCAGGATCCGCCAGGA CCTGGTCTTTCAGTACGTGGAGGACCCCACCATTGTGCGGATTGAACCAGAATGGAGCATTGTCAGTGGG AACACACCTATTGCTGTCTGGGGAACTCACCTGGACCTCATACAGAACCCACAGATCCGTGCCAAGCATG GAGGAAAAGAACACATCAACATCTGTGAGGTCCTAAATGCTACAGAGATGACCTGCCAGGCTCCAGCCCT TGCCCTGGGTCCCGACCACCAGTCAGATCTCACTGAGAGGCCTGAAGAATTTGGTTTCATCCTGGACAAT GTCCAGTCTCTGCTAATTCTCAACAAGACCAACTTCACCTATTATCCTAACCCCGTGTTTGAGGCTTTCA GTCCCTCGGGAATCCTGGAGCTCAAGCCGGGCACCCCCATTATCCTAAAGGGCAAGAACTTGATCCCGCC TGTGGCCGGAGGCAATGTGAAGCTGAACTACACCGTGCTGGTTGGGGAGAAACCATGCACCGTGACAGTA TCAGATGTGCAGCTGCTCTGCGAGTCTCCCAACCTCATCGGCAGGCACAAAGTGATGGCACGGGTGGGTG GCATGGAGTATTCCCCTGGGATGGTATACATCGCCCCAGACAGCCCGCTCAGCCTGCCCGCCATTGTCAG CATCGCGGTGGCTGGTGGCCTCCTCATCATCTTCATCGTGGCCGTACTCATCGCCTACAAACGCAAATCC CGAGAAAGTGACCTCACGCTGAAGAGGTTGCAGATGCAGATGGACAACCTTGAGTCCCGTGTGGCGCTGG AGTGCAAGGAAGCTTTTGCCGAGCTGCAGACAGATATCCATGAGCTTACCAGTGACCTGGATGGAGCTGG GATTCCCTTTCTGGACTACAGAACCTATACCATGCGGGTGCTGTTCCCAGGGATTGAAGACCACCCTGTG CTACGAGACCTTGAGGTCCCAGGCTACCGGCAGGAGCGTGTGGAGAAAGGACTCAAGCTCTTCGCCCAGC TCATCAACAACAAGGTGTTCCTGCTCTCTTTCATCCGCACTCTGGAGTCTCAGCGCAGTTTTTCCATGCG CGACCGAGGTAACGTGGCCTCCCTCATCATGACGGTACTTCAGAGCAAGCTAGAGTATGCCACTGACGTG CTGAAGCAGCTACTGGCTGACCTCATTGATAAGAACCTGGAGAGCAAGAACCACCCCAAGCTGCTGCTCA GGAGGACGGAGTCGGTGGCCGAGAAGATGTTGACTAATTGGTTCACTTTCCTCCTCTACAAGTTCCTCAA GGAGTGTGCTGGGGAGCCACTCTTCTCCCTGTTCTGTGCCATCAAGCAGCAGATGGAGAAGGGCCCCATT GATGCCATCACCGGAGAGGCCCGCTACTCGCTAAGTGAGGACAAACTCATCCGACAGCAGATTGAATATA AAACCTTGGTCCTGAGCTGTGTCAGTCCAGATAATGTTAACAGCCCAGAGGTCCCAGTGAAGATCCTCAA CTGTGACACCATCACTCAGGTCAAAGAGAAGATCCTAGATGCCATCTTCAAGAATGTCCCGTGCTCCCAT AGGCCCAAAGCTGCAGACATGGATCTAGAGTGGCGACAAGGAAGTGGGGCGAGGATGATCCTGCAGGACG AAGATATCACCACCAAGATCGAGAATGATTGGAAGAGACTCAACACAGTAGCCCACTACCAGGTACCAGA TGGTTCTGTGGTGGCTTTAGTGTCCAAGCAAGTGACAGCCTACAATGCAGTGAACAACTCCACTGTCTCC AGGACTTCAGCAAGTAAATATGAGAACATGATCCGGTACACAGGCAGCCCTGACAGCCTCCGTTCCCGGA CACCCATGATCACCCCTGACCTGGAAAGCGGGGTCAAGCTTTGGCATCTGGTGAAGAACCATGAACATGG AGACCAGAAGGAGGGGGACCGGGGGAGCAAGATGGTGTCTGAGATCTATCTGACACGACTATTGGCCACA AAGGGCACACTGCAGAAGTTTGTGGATGACCTATTCGAGACCATCTTCAGTACGGCCCACCGTGGCTCCG CTCTGCCGCTAGCCATCAAGTACATGTTTGACTTCCTCGATGAGCAGGCTGATAAGCACGGCATCCATGA CCCGCACGTCCGCCACACCTGGAAGAGCAACTGCCTGCCCCTACGGTTTTGGGTGAATATGATCAAGAAC CCTCAGTTTGTGTTTGACATCCATAAGAACAGCATCACGGATGCCTGTCTCTCTGTGGTGGCCCAGACCT TCATGGACTCCTGCTCCACTTCGGAGCACCGACTGGGCAAGGATTCCCCCTCCAACAAGCTGCTCTATGC CAAGGACATCCCCAGCTACAAGAACTGGGTAGAGAGGTATTATTCAGACATTGGGAAGATGCCGGCAATA AGTGACCAGGACATGAATGCATACCTGGCCGAGCAGTCGCGGATGCACATGAATGAGTTCAATACGATGA GTGCGCTCTCAGAGATCTTCTCCTATGTGGGCAAATACAGTGAGGAGATCCTTGGACCCCTGGACCATGA TGACCAGTGTGGAAAGCAGAAACTGGCTTACAAACTAGAACAAGTCATAACTCTCATGAGCTTAGACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226328 representing NM_175750
Red=Cloning site Green=Tags(s) MPWNWTCLLSHLLVVGMGSSTLLPRQPPQLSQKPSFVTFRGEPAEGFNHLVVDERTGHIYLGAVNRIYKL SSDLKVLVTHQTGPDEDNPKCYPPRIVQTCNEPLASTNNVNKMLLIDYKENRLIACGSLYQGICKLLRLE DLFKLGEPFHKKEHYLSGVNESGSVFGVIVSYSNFDDKLFIATAVDGKPEYFPTISSRKLTKNSEADGMF AYVFHDEFVASMIKIPSDTFTVIPDFDIYYVYGFSSGNFVYFLTLQPEMVSPPGSTTKEQVYTSKLVRLC KEDTAFNSYVEVPIGCERNGVEYRLLQAAYLSKAGAVLGRTLGVRPDDDLLFTVFSKGQKRKMKSLDESA LCIFILKQINDRIKDRLQSCYRGEGTLDLAWLKVKDIPCSSALLTIDDNFCGLDMNAPLGVSEMVRGIPV FTEDRDRMTSVIAYVYKNHSLAFVGTKSGKLKKIRVDGPKGNALQYETVQVVDSGPVLRDMAFSKDHEQL YIMSERQLTRVPVESCGQYRSCGECLGSGDPHCGWCVLHNTCTRKERCERSREPRRFASEMKQCVRLTVH PNNISVSQYNVLLVLETYNVPELSAGVNCTFEDLSEMDGLVIGNQIQCYSPAAKEVPRIITENGDHHVVQ LQLKSKETGMTFASTSFVFYNCSVHNSCLSCVESPYRCHWCKYRHVCTHDPNTCSFQEGRVKLPEDCPQL LRVDKILVPVEVIKPITLKAKNLPQPQSGQRGYECILNIQGIEQRVPALRFNSSSVQCQNTSYSYEGMEI NNLPVELTVVWNGHFNIDNPAQNKVYLYKCGAMRESCGLCLKADPDFECGWCQSPGQCTLRQHCPAHESR WLELSGANSKCTNPRITEIIPVTGPREGGTKVTIRGENLGLEFRDIASHVKVAGVECSPLVDGYIPAEQI VCEMGEAKPSQHAGFVEICVAVCRPEFMARSSQLYYFMTLTLADLKPNRGPMSGGTQVTITGTNLNAGSN VVVMFGSQPCLFHRRSPSYIICNTTSSEEVLDMKVTVQVDRARIRQDLVFQYVEDPTIVRIEPEWSIVSG NTPIAVWGTHLDLIQNPQIRAKHGGKEHINICEVLNATEMTCQAPALALGPDHQSDLTERPEEFGFILDN VQSLLILNKTNFTYYPNPVFEAFSPSGILELKPGTPIILKGKNLIPPVAGGNVKLNYTVLVGEKPCTVTV SDVQLLCESPNLIGRHKVMARVGGMEYSPGMVYIAPDSPLSLPAIVSIAVAGGLLIIFIVAVLIAYKRKS RESDLTLKRLQMQMDNLESRVALECKEAFAELQTDIHELTSDLDGAGIPFLDYRTYTMRVLFPGIEDHPV LRDLEVPGYRQERVEKGLKLFAQLINNKVFLLSFIRTLESQRSFSMRDRGNVASLIMTVLQSKLEYATDV LKQLLADLIDKNLESKNHPKLLLRRTESVAEKMLTNWFTFLLYKFLKECAGEPLFSLFCAIKQQMEKGPI DAITGEARYSLSEDKLIRQQIEYKTLVLSCVSPDNVNSPEVPVKILNCDTITQVKEKILDAIFKNVPCSH RPKAADMDLEWRQGSGARMILQDEDITTKIENDWKRLNTVAHYQVPDGSVVALVSKQVTAYNAVNNSTVS RTSASKYENMIRYTGSPDSLRSRTPMITPDLESGVKLWHLVKNHEHGDQKEGDRGSKMVSEIYLTRLLAT KGTLQKFVDDLFETIFSTAHRGSALPLAIKYMFDFLDEQADKHGIHDPHVRHTWKSNCLPLRFWVNMIKN PQFVFDIHKNSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIPSYKNWVERYYSDIGKMPAI SDQDMNAYLAEQSRMHMNEFNTMSALSEIFSYVGKYSEEILGPLDHDDQCGKQKLAYKLEQVITLMSLDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_175750 |
ORF Size | 5670 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175750.1, NM_175750.2 |
RefSeq Size | 12602 bp |
RefSeq ORF | 5682 bp |
Locus ID | 243743 |
UniProt ID | Q80UG2 |
MW | 213 kDa |
Gene Summary | Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.[UniProtKB/Swiss-Prot Function] |
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